囊胚

Embryonic stem cell has the capability of immortalization and pleuripotent differentiation.

胚胎干细胞源于着床前的囊胚内细胞团或早期胎儿原始生殖细胞的一类未分化的多能性干细胞，具有无限增殖和多能分化的能力。

Objective: We try to elucidate the probable mechanism of midblastula transition in zebrafish through study the cyclin C gene which is expressed maternally and zygotically during zebrafish embryo development.

斑马鱼胚胎发育过程中cyclinC基因母型和合子型表达的初步研究目的：通过研究cyclin C基因在母型和合子型的表达研究，阐释其在中囊胚过渡时期的可能作用。

Of a fertilized ovum and consisting of a spherical layer of cells surrounding a fluid-filled ''.

囊胚由分裂受精卵产生的早期胚胎外形，由围绕充满液体的''。

Results showed that sulfapyridine, tricyclazole, sulfadiazine and imazalil at low concentration（0.01mg·L-1） have the highest relative lethality rate to swelling of insemination membrane, two cells, swimming blastula and prism stage, respectively, which were 3.70%, 6.19%, 5.84% and 6.07%, respectively.

实验浓度下，磺胺吡啶、三环唑、磺胺嘧啶和抑霉唑分别对受精膜举起期、2细胞期、上浮囊胚期和棱柱幼体期相对致死率最高，分别达到3.70%、6.19%、5.84%和6.07%。

Embryonic stem cells derived from inner cell mass of mammalian blastocyst-stage emyst-stage embryos and primordial germ cells are totipotent cells that can be cultured in virto.

胚胎干细胞是从哺乳类动物的囊胚内细胞群或原始生殖嵴细胞分离、体外培养获得的具有发育全能性的细胞。

It is also an ideal source of tissue engineering and cell substitutive therapy of regenerative medicine. The target of this experiment is to investigate how to isolate totipotent mouse ESCs from BALB/C species mice and to analysis some biological characteristics of ESCs.

本实验旨在研究如何从BALB／C小鼠的囊胚中分离出具有全能性的胚胎干细胞，并对胚胎干细胞集落进行培养、鉴定，以了解胚胎干细胞生物学特性，为掌握小鼠ESCs分离培养技术及将来进一步研究人ESCs打下基础。

AIM To study the isolation and in vitro culture of rabbit s bone mesenchymal stem cells and adipose stromal stem cells and the use of both BMSCs and ASSCs cells in nuclear transfer to produce totipotent blastocysts.

目的 研究非休眠期成年兔骨髓基质干细胞和脂肪基质干细胞作为供体，以体外成熟去核兔卵母细胞作为受体进行核移植构建重组胚胎的可行性及囊胚形成率。

The method of vitrification cryopreservation of mouse 2-cell embryos was studied. Several vitrification solutions named EFS30, EFS40, EDFS30 and EDFS40 were prepared. The solution contains ethylene glycol, Ficoll, sucrose and DMSO. Straw method was used to vitrify and cryopreservate in vivo mouse 2-cell embryos at room temperature (20℃ or 25℃). OPS method was used to vitrify the embryo at 25 ℃ or 37℃.

本试验以毒性低、渗透性较强的乙二醇和DMSO为主体，添加聚蔗糖、蔗糖配制的玻璃化溶液(EFS30、EFS40、EDFS30和EDFS40)，在室温(20℃和25℃)下利用细管法，以及在25℃和37℃恒温台条件下OPS法对小鼠2-细胞胚胎进行玻璃化冷冻保存，并对解冻后胚胎的体外培养、囊胚细胞计数及胚胎移植技术进行了研究。

Subsequently,with the straw and the cell freezing tube,we usedone-step and two-step method to vitrify the embryo of Lats gene knockout mice.The development ratio reached 90% 90.1 % 89.2%, 91.4% and the implantation rauo reached 50.0%, 47.1% 43.4%, 46.5% respectively after thaw.

在此基础上，用冷冻麦管、细胞冷冻管分别采用一步法、二步法对Lats基因敲除小鼠胚胎进行玻璃化冷冻，胚胎解冻后，囊胚发育率分别为：90％、90.1％、89.2％、91.4％；移植后着床率分别：50.0％、47.1％、43.4％、46.5％，差异不显著；并且与对照组相比差异也不显著。

Three types of cells were transferred into perivitelline space under zona pellucida of rabbit oocytes respectively.

用体外培养14代和24代的小鼠胚胎干细胞构建的异种重构卵的卵裂率分别是88.5％和82.1％(P＞0.05)，囊胚发育率分别是16.7％和15.4％(P＞0.05)。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。