囊胚

The colony of embryonic stem cell separated from blastula could gain five strains of embryonic stem cell and go down to posterity F7 in vitro.

从培养的囊胚中分离获得的类胚胎干细胞集落，经传代培养获得了5株胚胎干细胞株，在体外可传代培养到7代，冷冻保存复苏后，干细胞仍能存活、增殖。

AIM:Studies on totipotential stem cells using primordial germ cells have the same application prospect as embryonic stem cells, especially for the animals difficult to isolate embryonic stem cells from blastula.

目的：应用原始生殖细胞进行全能干细胞的研究具有与胚胎干细胞同样广阔的前景，尤其适合于难以从囊胚内细胞团中分离培养胚胎干细胞的动物。

Double-walled stage of the embryo succeeding the blastula.

囊胚发育之后的双层胚胎。

The oocytes were divided into slow-freezing group and vitrification group, according to the cryopreservation methods. And then the vitrification group was divided into self-made up solution group and commercial solution group, and the survival rate, fertilization rate, cleavage rate and blastulation rate of all groups were compared.

根据冷冻方法分为程序化冷冻组和玻璃化冷冻组，又将玻璃化冷冻组分为自配液冷冻组和成品液冷冻组，比较各组存活率、受精率、卵裂率及囊胚形成率。

Methods: Human mature oocytes were cryopreserved using cryoleaf. Resuscitated oocytes were administered by intracytoplasmic sperm injection, embryo transplantation or cultured to blastulation, observing the fertilization and developmental capacity of oocytes.

运用cryoleaf进行人类成熟卵母细胞玻璃化冷冻保存，复苏后行胞浆内单精子注射-胚胎移植或培养至囊胚形成，观察复苏后卵母细胞受精及发育能力。

Methods: MII, GV and IVM-MII were vitrified. After thawed, they underwent in vitro maturation, in vitro fertilization, embryo cultivation or fixed for immunofluorescence. The rates of survival, maturation, fertilization, blastulation and cytoskeleton with normal configuration were calculated.

玻璃化冷冻生殖泡期卵、成熟中期卵及体外成熟卵，解冻复苏后，分别作体外成熟、体外受精、胚胎培养或固定作免疫荧光标记，统计复苏率、成熟率、受精率、囊胚率、细胞骨架正常率。

Methods: mⅱ,gv and ivm-mⅱ were vitrified. after thawed, they underwent in vitro maturation, in vitro fertilization,embryo cultivation or fixed for immunofluorescence. the rates of survival, maturation, fertilization, blastulation and cytoskeleton with normal configuration were calculated.

玻璃化冷冻生殖泡期卵、成熟中期卵及体外成熟卵ivm-mⅱ），解冻复苏后，分别作体外成熟、体外受精、胚胎培养或固定作免疫荧光标记，统计复苏率、成熟率、受精率、囊胚率、细胞骨架正常率。

Subsequently cellular become divided brings about a series of " kind the formation of bursa embryo ".

随后的细胞分化则导致一系列"类囊胚"的形成。

In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期，高度表达的合子转录本仍在所有的胚盘细胞中，但至90%下包期时，pou2的表达向胚胎背部的正中线汇聚，集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时，pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时，转录本消失，这与RT-PCR结果高度一致。

Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed from blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells.

我们用RT-PCR和整体原位杂交的方法研究了银鲫pou2基因在胚胎发育过程中的时空表达图式RT-PCR结果显示，银鲫pou2基因有母源转录本，其合子基因在高囊胚期强烈表达，在50%下包期和90%下包期也有高量的转录本，但在100%下包期表达量急剧降低，至体节期时已经完全检测不到其转录本胚胎整体原位杂交结果显示其母源转录本在所有的胚盘细胞中。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。