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Membrane remodelling1, 2, 3, 4, 5 plays an important role in cellular tasks such as endocytosis, vesiculation and protein sorting, and in the biogenesis of organelles such as the endoplasmic reticulum or the Golgi apparatus.

生物谷报道:细胞膜远不止是细胞外的一层包裹物,对其拓扑进行的重塑研究,将它们与内吞作用、囊泡形成和蛋白分选等重要功能联系了起来。

Subsequently,with the straw and the cell freezing tube,we usedone-step and two-step method to vitrify the embryo of Lats gene knockout mice.The development ratio reached 90% 90.1 % 89.2%, 91.4% and the implantation rauo reached 50.0%, 47.1% 43.4%, 46.5% respectively after thaw.

在此基础上,用冷冻麦管、细胞冷冻管分别采用一步法、二步法对Lats基因敲除小鼠胚胎进行玻璃化冷冻,胚胎解冻后,囊胚发育率分别为:90%、90.1%、89.2%、91.4%;移植后着床率分别:50.0%、47.1%、43.4%、46.5%,差异不显著;并且与对照组相比差异也不显著。

Results The tumors were divided into three types according to the CT findings. Type I: regular, homogeneous density and well-defined,there were 30 patients, included benign mixed tumor(n=29) and adenolymphomas(n=1); Type II:irregular, heterogeneous dentsity, poordefined, there were 15 patients, included benign mixed tumor(n=7), malignant mixed tumor (n=2),adenolymphomas(n=3), hemagioma(n=2),mucoepidermoid carcinoma(n=1). Type III: irregular and could not definded, there were 7 patients, included malignant mixed tumor(n=2), mucoepidermoid carcinoma(n=2), adenoid cystic carcinoma(n=1), malignant lymphoma(n=1), acinic cell carcinoma(n=1). Conclusion CT have important values in location and qualitative analyses of parotid gland.

结果 肿瘤按CT表现分三类:52例患者中,I类30例,形态规则呈类圆形,密度均匀,边缘清楚,除1例腺淋巴瘤外,29例均为良性混合瘤;II类15例,形态不规则,呈分叶状,密度欠均,边界较清或模糊,其中7例良性混合瘤,2例恶性混合瘤,3例腺淋巴瘤,2例血管瘤,1例黏液表皮样癌;III类7例,形态不规则,边缘无法确定,其中恶性混合瘤2例,黏液表皮样癌2例,囊腺癌1例,恶性淋巴瘤1例,腺泡细胞癌1例。

Furthermore, we encapsulated CEA transgeneic cells with micorencapsule that is made from sodium alginic acid and chitin.

进一步用以海藻酸钠和壳聚糖为成囊材料的微胶囊包裹转染CEA基因的细胞。

OBJECTIVE: To observe the changes of myelin sheath in the injured transection of rats after transplantation of the alginic acid microencapsulated Schwann cells.

目的:将海藻酸钠微囊包被兔许旺细胞后移植于大鼠脊髓损伤处,观察移植后髓鞘结构的变化。

Firstly , the V4 stock was purified three times by plaque formation. It's difficult to see clear plaques in CEF cells due to the avirulent characteristic , so we treated the V4 stock with trypsin and then allantoic Fluid and Mg2+ were added in the layer gel. In this case, we could see small plaques occasionally, but not stably.

本实验首先对实验室保存的V4株进行了三轮的蚀斑纯化,由于V4株属无毒株,其在CEF细胞中很难形成可见的蚀斑,因此我们采用了用胰酶处理V4病毒,并在上层胶中添加尿囊液及Mg2+,这样可以偶尔产生可见的蚀斑,但实验结果重复性很差。

METHODS: From January 2000 to May 2002, 52 cases with various types of bone defect were treated with tissue engineered bone, which was constructed in vitro by allogeneous osteoblasts from periosteum 1 x 10(6/ml with bio-derived bone scaffold following 3 to 7 days co-culture. Among them, there were 7 cases of bone cyst, 22 cases of non-union or malunion of old fracture, 15 cases of fresh comminuted fracture of bone defect, 4 cases of spinal fracture and posterior route spinal fusion, 3 cases of bone implant of alveolar bone, 1 case of fusion of tarsotarsal joint.

2000年1月~ 2002年1月,用同种异体骨膜来源的成骨细胞 1×106 / ml与生物衍生骨支架材料构建的组织工程骨,经体外培养 3~ 7天后,植入修复52例各种类型的骨缺损,其中骨囊性病变7例,陈旧性骨折不愈合或畸形愈合 2 2例,新鲜粉碎性骨折伴骨缺损 15例,脊柱骨折后路植骨融合术4例,牙槽骨植骨 3例,足跗中关节植骨融合 1例。

BACKGROUND: Compared with adult mesenchymal stem cells, yolk sac-derived MSCs of early embryo have stronger self-renewal capacity and plasticity. Moreover, they could differentiate into angioblast and hemopoietic stem cells during embryonic development and trigger embryonic angiogenesis and hemopoietic tissue development.

背景:与成体间充质干细胞相比,来源于早期胚胎的卵黄囊间充质干细胞有更强的自我更新能力和可塑性,且在胚胎发育过程中可分化为成血管细胞和造血干细胞,启动胚胎血管发生和造血组织发育。

In the"poly-egg apparatus embryo sac",three egg-apparatuses are the majority which canbe further divided into three subtypes,namely,"5-2-1"type,"6-2-0"type and"5-3-0"type,based on the position of antipodal cell.

三卵卵器结构胚囊&依反足细胞的位置又可分出&5-2-1&型、&6-2-0&型和&5-3-0&型等三种亚类型。&

Could be observed on 10th day after pollination, archesporial cell on 20thday, megasporocyte on 24th day, the functional megaspore on 30th day; and 8-nucleate embryo sac on 41th day. And then double fertilization finish and early globular embryo emerg.

授粉10 d出现指状突起,20 d形成胚珠原基,24 d大孢子母细胞发生,30 d功能大孢子形成,41 d发育为成熟的八核胚囊,而后完成双受精发育成早期球形胚。

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