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In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期,高度表达的合子转录本仍在所有的胚盘细胞中,但至90%下包期时,pou2的表达向胚胎背部的正中线汇聚,集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时,pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时,转录本消失,这与RT-PCR结果高度一致。

Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed from blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells.

我们用RT-PCR和整体原位杂交的方法研究了银鲫pou2基因在胚胎发育过程中的时空表达图式RT-PCR结果显示,银鲫pou2基因有母源转录本,其合子基因在高囊胚期强烈表达,在50%下包期和90%下包期也有高量的转录本,但在100%下包期表达量急剧降低,至体节期时已经完全检测不到其转录本胚胎整体原位杂交结果显示其母源转录本在所有的胚盘细胞中。

The influences of INA were as follows: The mulcher of pollen parietes was disintegrated or disappeared in certain section and even the entire extine was deleted. The injuries of organelles in the pollen were demonstrated by plastosome peplos imperfect, vesicle sterility; concentric circles of the endoplasmic reticulum or fold arranged, to form the membranal inclusions by enwrapping part of the cytoplasm, and decrease of ribosome amount.

INA细菌对花粉的超微结构的影响表现为:使花粉壁的覆盖层在某些区域解体消失或整个花粉外壁缺失;使花粉中的细胞器受到破坏,包括线粒体被膜不完整,嵴呈囊泡状;内质网呈同心圆卷绕或折叠状排列包围部分细胞质而形成膜内含物;核糖体数量明显减少等。

The dynamic changes of IgM, IgG, IgA B lymphocytes, namely, the rule of their appearance, tissue localization and distribution in the bursa of Fabricius, were studied by the immunohistochemistry method.

利用免疫组织化学法研究了IgM、IgG和IgA三种B细胞在法氏囊内的出现、组织定位及分布变化规律。

Objective: To investigate the effect of basic fibroblast growth factor on matrix metalloproteinases and restenosis after PTCA.

目的:探讨碱性成纤维细胞生长因子对基质金属蛋白酶表达的影响及其在球囊术后再狭窄中的作用。

Benign cystic teratomas are well-differentiated tumors composed of a variety of parenchymal cell types representative of more than one germ layer, usually all three. The most common locations are the gonads and sacrococcygeus.

成熟囊状畸胎瘤是一种良性且分化良好的肿瘤,由胚胎原始细胞分化而成,由多於一层胚层之组织而成;好发位置为性腺体、骶骨与尾骨部或纵隔腔,十分罕见於头颈部。

Objective To observe the effects of microencapsulated rabbit sciatic nerve tissue transplantation on substance P expression in dorsal root ganglia after spinal cord hemisection injury.

目的 观察微囊化兔坐骨神经组织细胞移植对脊髓半横断后脊神经节P物质表达的变化。

Methods:33 cases were classified asⅠ,Ⅱ,Ⅲ according to heteromorphic degree of interstitial cells,nuclear fission and special interstitial ingredient.

对 33 例经病理证实的乳腺叶状囊肉瘤,根据间质细胞异形程度和核分裂数、是否浸润、是否含有特殊间质成分进行分级。

These cysts are lined by epithelium with eosinophilic cytoplasm and a hobnail morphology, and there is often ovarian-like stroma.

囊壁由含有嗜酸性胞浆的上皮围成,上皮细胞呈鞋钉状,常常有卵巢样间质。

Grossly, the tumors ranged from 0.7 to 17 cm, and exhibited a spongy or bubble wrap appearance reflecting the microscopic presence of variably sized cystically dilated tubules lined by a single layer of epithelium. The lining varied with a cuboidal, flat, and hobnail cell appearance, and the neoplastic cells had abundant eosinophilic cytoplasm and enlarged nuclei with prominent nucleoli.

大体上,肿瘤大小为0.7至17cm,海绵样或泡沫样外观,镜下观是大小不等的扩张成囊的管子,这些管子被覆单层上皮,上皮形态立方状、扁平状和鞋钉样,肿瘤细胞胞浆丰富、嗜酸,细胞核大,核仁明显。

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主要做了如下几个方面的工作:对伸臂位于顶部的单层框架—筒体模型进行分析,提出了等效转动约束的概念和转动约束刚度的表达式。

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