囊细胞

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

The embryonic vessels failed to invade into the labyrinthine layer of placenta, which impaired the embryonic-maternal vascular connection. These defects could not be rescued by wild-type tetraploid blastocysts, excluding the possibility that they were due to the extraembryonic tissues.

组织免疫组化分析结果显示：毛细血管腔异常扩张，排列紊乱，不能形成大血管及细致有序的血管网络；胚外中胚层来源的卵黄囊脏层扁平上皮细胞形态变圆，与内皮细胞连接受损；胚胎血管不能侵入胎盘迷路层，胚体与母体血管连接受损。

The conclusion showed that the yeast two hybrid cDNA library of chicken B cells Bursa of Fabricius was successfully constructed by CLONTECH SMART method in yeast cells.

研究结论表明可在酵母细胞中利用CLONTECHSMART技术成功构建鸡法氏囊B细胞的酵母双杂交cDNA文库。

In the late of April of the second year, male inflorescences unbound and pollinated. When female inflorescences accepted the pollen grains, ovary development was increased in early May. With the development of either of the two ovule, funiculus, integument and nucellus were differentiated. Embryo sac mother cell came into being and the meiosis begins. Then fuctional megaspore developed into matured embryo sac that contain eight nucleuses. In the end of May, double fertilization generated.

次年，4月下旬，雄花序解螺旋散粉，雌花序开花授粉；5月上旬，雌花序授粉后，迅速增长，子房膨大，两个胚珠分化出珠柄、珠被和珠心，珠心中产生孢原细胞；5月中下旬，大孢子母细胞形成并减数分裂，功能性大孢子形成成熟的八核胚囊；5月底，发生双受精。

Female gametogenesis in higher plants undergoes various developmental changes, including the de novo formation of germline cells, the differentiation of the megaspore mother cell, meiosis, megaspore selection, and the development of the embryosac.

高等植物雌配子体的形成涉及孢原细胞和大孢子母细胞的确立与分化、大孢子发生、功能大孢子以及胚囊的形成和发育等多种复杂调控过程。随着当代生物技术及功能基因组学的发展，近年对雌配子体发育的研究已从细胞学描述逐渐过渡到对基因和发育调控分子机理的探索。

Results After oral administration of ST pinocytosis vesicles began to increase in endothelial cells of blood vessel in 1 hour,apocrine of ependymal cells began to increase and myelin sheath degeneration appeared in 2 hours,extensive myelin sheath degeneration and mitochondrion degeneration and incisure and vacuoles in nucleus of neuron were found in 4 and 8 hours.

结果 ST灌胃后1 h血管内皮细胞吞饮囊泡开始增多，2 h组室管膜细胞的顶浆分泌增多，部分神经髓鞘变性；4 h和8 h组神经纤维广泛变性，神经元线粒体变性、核切迹和空泡出现；16 h组神经元胞浆内线粒体变性更加明显，并出现脂褐素。

In order to fuse, intra cell ular vesicles have to move within the cell

为了融合，细胞内的囊泡必须在细胞内运动。

In order to fuse , intracellular vesicles have to move within the cell.

为了融合，细胞内的囊泡必须在细胞内运动。

Among them, 22 cases were clear cell carcinoma, 3 cases were chromophobe cell cancer, 2 cases were mammilla kidney cancer and 1 case was capsule kidney cancer.

结果：术后均未发生严重并发症，术后病理均为T1a期，其中透明细胞癌22例，嫌色细胞癌3例，乳头状肾癌2例，囊性肾癌1例。

Among the 20 samples of IGF- 1 group, a 8-cell stage embryo, a morula and two blastula were found as positive by the red colour appearance .

IGF上的表达 2～8细胞胚胎 18个，桑堪胚 1个，囊胚 2个，共计 ZI个胚胎用于＊F2 检测，阳性表达14个，其中强阳性表达7个，弱阳性表达7个，分布于各阶段胚胎，在胚胎细胞中呈现深、浅程度不同的橘红色染色。l级和 2级胚胎 IGFZmRNA的阳性表达差异有显著性。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。