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The cleavation rates and blastocyst development rates of cloned embryos were used to assess the efficiency of different operational procedure. Finally, the best combination of operational procedure, that the spindle-viewer system was used for oocytes enucleating, and donor cell was electrofused into ooplasm by electrical pulse (1.9 kV/cm, 10 ms, two) to reconstruct bovine cloned embryos.

以核移植胚胎的卵裂率、囊胚发育率作为检测指标,对不同的方法所获得的克隆胚胎的卵分裂率与囊胚发育率进行比较,最后筛选获得1个优化的牛体细胞核移植操作程序,即采用Spindle view系统对牛卵母细胞进行去核操作,将供核体细胞注射到卵周隙,然后通过电融合法将供体核引入去核卵细胞质(电融合参数为1.9 kV/cm,脉冲时程10 ms,方波2次间隔2 s)。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示:未妊娠时,泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达,波形蛋白在子宫内膜基质细胞内表达,平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达,牦牛子宫任何部位均不表达角蛋白7;妊娠30天左右时,泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达,波形蛋白在子宫内膜基质细胞和内胚层细胞内表达,平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达,角蛋白7在尿囊细胞内表达,偶尔在腔上皮细胞的细胞核边缘表达;消化法进行原代培养时,组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞;分离得到的子宫内膜基质细胞活率达90%以上,并可在体外传代7次以上;分离得到的子宫内膜腺上皮细胞活率可达85%以上,并可在体外传代5次以上;RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长,维持子宫内膜基质细胞正常生长的FBS添加量为20%,维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

Genetic analysis of soybean resistant to soybean nematode race 5. Crop Sci., 1989, 29:1181~1184.[4]Qiu B, Sleper D A et al. Inheritance of resistance to Heterodera glycines races 1,3, and 5 in Peking. Soybean Gen. Newsl., 1996, 23:183~185.[5]Rao-Arelli A P. Allelic loci in Peking soybean controlling resistance to Heterodera glycines races. Soybean Gen. Newsl., 1995, 22:170~172.[6]Rao-Arelli A P. Inheritance of resistance to Heterodera glycines race 3 in soybean accessions. Plant Dis., 1994, 78:898~900.[7]Golden A M, Epps J M, Riggs R G et al. Terminology

随机选取30个F3代的株系,每个株系随机选取10~15个单株,进行上述孢囊线虫的接种鉴定,设抗感亲本作对照。8个株系抗病,18个株系分离和4个株系感病。F3代株系间的抗感分离比例确认兴县灰布支黑豆对大豆孢囊线虫14号小种的抗性是由3对基因控制、一对隐性和两对显性基因、隐性基因上位、两对显性基因互补的遗传假设是正确的。

These two kind of diseases have their own characteristics in clinical and imaginal representation.but misdiagnosis is still happened.

乳腺纤维腺瘤与乳腺叶状囊肉瘤在临床和影像学表现方面都有其特征性,但临床上仍常出现误诊的情况,我们于2002年4月收治1例发病于妊娠哺乳期的28岁右乳巨大肿块女性病人,术前根据临床表现及影像学检查的结果考虑右乳叶状囊肉瘤的诊断,而术后的病理报告为乳腺纤维腺瘤。

"Fetterbush:any of several deciduous or evergreen shrub s of the genus Leucothoe, having clusters of white to pink flowers and many-seeded capsules with five valves."

&亮叶南烛:几种落叶的或常绿的灌木属中的一类木黎芦属,长有成簇的介于白色和粉色之间的花和五片囊盖许多粒种子的种囊。&

"Fetterbush:any of several deciduous or evergreen shrubs of the genus Leucothoe, having clusters of white to pink flowers and many-seeded capsule s with five valves."

&亮叶南烛:几种落叶的或常绿的灌木属中的一类木黎芦属,长有成簇的介于白色和粉色之间的花和五片囊盖许多粒种子的种囊。&

"Fetterbush:any of several deciduous or evergreen shrubs of the genus Leucothoe, having clusters of white to pink flowers and many-seeded capsules with five valves."

&亮叶南烛:几种落叶的或常绿的灌木属中的一类木黎芦属,长有成簇的介于白色和粉色之间的花和五片囊盖许多粒种子的种囊。&

Any of several deciduous or evergreen shrubs of the genus Leucothoe, having clusters of white to pink flowers and many-seeded capsules with five valves.

亮叶南烛几种落叶的或常绿的灌木属中的一类木黎芦属,长有成簇的介于白色和粉色之间的花和五片囊盖许多粒种子的种囊

In filicophytes, there was a relatively high ratio of VA mycotrophy in the eusporangiates, and there was no autotrophic in the eusporangiates; but the ratio of autotrophic was up to 71%, and the ratioes of VA mycotrophic and facultative mycotrophic were low, 15% and 14% respectively in the leptosporangiates.

用碱解离、酸性品红染色法对云南热带、亚热带自然生长和部分移栽到温室中的256种(拟蕨类植物20种,真蕨类植物236种)蕨类植物孢子体的VA菌根状况进行了调查研究,结果发现,在拟蕨类植物中,孢子体只存在兼性VA菌根营养和自养两种菌根营养类型,两种类型,两种类型所占的比例相当;在真蕨类植物中,厚囊蕨类植物VA菌根营养的比例较高(92%),且无自养类型;而在薄囊蕨类植物中,自养型占71%,VA菌根营养和兼性菌根营养所占的比例都不高,分别是15%和14%。

With the prolonging of treatment with 6-DMAP,the developmental ability of parthenogenetic embryos decreased.

随着6-DMAP处理时间加长,孤雌胚的发育能力降低。6-DMAP处理1小时的桑葚胚/囊胚的发育率(72%)远远高于6-DMAP处理6小时的桑葚胚/囊胚的发育率(28%)。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。