囊的

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Observing the mature embryo sac of the mutant: It was found thatthe embyo sac appeared in typical polygonum type and more than 90% of the embryo sac hadintegral inner structure. They had egg apparatus, two polar nucleus and three antipodal cells , and their position in the embryonic sac was normal .

幼穗分化、成熟胚囊观察发现，90％以上的胚囊具有完整的内部结构，有卵器、两个极核及多个反足细胞，且位置正常，胚囊呈现典型的蓼型结构；同时还观察到雌、雄器官的发育是同步的。

2 Treatment method is less than a month treat a little patient to use method of massage drop medicine first, treat 1~2 week, 2~3min is massaged before daily drop medicine, be more than the little patient of a month, if drop medicine treats 1~2 week to did not see,improve to still have apparently weep the person that reach secretion, applied medicaments pressurization rinses lachrymal path, use pinhead of vein of skin of tube head of nylon of self-restrained and different type to grind blunt, make rinse a needle continuously, groovy operation, after syringe needle inserts lachrymal canaliculus, nylon the canal is secured with adhesive plaster at the head, undertake rinsing, 1 / D, with oxygen fluorine to lotion Sha Xing adds loose diluent of rice of ground a place of strategic importance, pressurization rinses 3~4 second, invalid still, undertake probing of groovy tear path is operated.

我科自2000年1月至2006年12月，共治疗新生儿泪囊炎178例、230眼，疗效满足，报告分析如下。1资料和方法1.1一般资料新生儿泪囊炎患儿168例、230眼，男102例，女66例，年龄：2d~3个月，均为本院眼科门诊确诊的新生儿泪囊炎患儿。1.2治疗方法小于1个月的首次就治患儿采用按摩滴药法，治疗1~2周，每日滴药前按摩2~3min，大于1个月的患儿，如滴药治疗1~2周未见明显好转仍有流泪及分泌物者，应用药物加压冲洗泪道，采用自制不同型号尼龙管头皮静脉针头磨钝，制成直的冲洗针，常规操作，针头插入泪小管后，将尼龙管用胶布固定于头部，进行冲洗，1次/d，冲洗液用氧氟沙星加地塞米松稀释液，加压冲洗3~4次，仍然无效的，进行常规泪道探通术操作。1.3治疗标准无流泪、无分泌物、泪道冲洗畅通，反之则无效，结果168例，230眼，治愈166例，228眼，其中按摩治疗49例，冲洗86眼，探通31眼。。。

Results 4.16×10 9 oocysts were obtained in 30 mice in the 3rd group with dexamethasone of 20 μg/ml in drinking water. No significant difference in the oocyst recovery, purity and infectivity was found between methods using saturated saline floatation and sucrose density gradient centrifugation. The infectivity of the oocysts obtained from the same origin but different animals was similar.

结果 地塞米松加入量为 2 0 μg/ml的第 3组获得的卵囊量高达 4 。16× 10 9/ 30个小鼠；用饱和盐水漂浮法与用蔗糖密度梯度离心法纯化得到的卵囊具有相同的回收率和纯度；从感染小鼠与犊牛中获得的卵囊在感染性上差异不显著。

Results:Main imaging features of APK were as follows:On abdominal plain films showed the lobalate renal enlargement in 46 cases,calcification of polycystic wall in 14 cases,renal calcium milk in 5 cases,60 cases were showed separation,deform and longthend of renal pelvis on intravenous urography and retrograde urography,5 cases were showed renal enlargement on retroperitoneal pneumography,on CT scans in 25 cases showed mulltiply cysts with thin wall,unecho liquid shadows were showed on B-mode ultrasound.

结果：成人多囊肾的主要影像学征象：腹部平片(46例)显示肾影呈分叶状增大；囊壁钙化(14例)；多囊肾合并肾钙乳(5例)；肾盂造影(60例)，表现为肾盂、肾盏相互分离、变形、拉长；腹膜后充气造影(5例)：表现为肾影增大肾影与邻近器官之间的关系，其界限清晰可辨；CT扫描(25例)：表现为肾内多数大小不等的薄壁圆形低密度区；B型超声(47例)表现为肾内多个类圆形无回声的液性暗区。

The monotypic genus Macrovalsaria previously of uncertain taxonomic position turns out to be a member of Botryosphaeriales and seems to be related to Botryosphaeria. The placement of Neopeckia and of Dothidotthia is in Dothideales and Botryosphaeriales, respectively. The genera Plowrightia and Botryosphaeria as currently defined are not monophyletic. Species of Plowrightia appear to be associated with three genera, Dothidea, Neopeckia and Sydowia; those of Botryosphaeria tested are recognized as three groups.

结果还显示，座囊菌纲中分类地位不确定的属Macrovalsaria与Botryosphaeria的关系相对接近，应该归于葡萄座腔菌目；历史上分类地位有疑问的Neopeckia和Dothidotthia两个属处于不同的目中，前者属于座囊菌目，后者为葡萄座腔菌目的成员；座囊菌目的Plowrightia属并非单系群，该属成员分别与Dothidea、Neopeckia和Sydowia三个属聚类在一起；现行的Botryosphaeria属也非单系群，其属的概念有待澄清。

The present study found that the contents of ferromagnetic elements in lagenal otoliths of adult homing pigeons were less than 0.7% and were the same order in magnitude as those in saccular and utricular otoliths.

本研究的结果发现：成年信鸽瓶状囊耳石中铁磁性元素Fe、Co、Ni的含量不足Ca元素的0.7%，与球囊和椭圆囊耳石中的含量为同一数量级。

A follow-up ultrasound reealed a second, smaller septated cystic structure- a mirror image of the first- anterior to the initial cystic structure and posterior to the bladder.

超声复查发现了第二个小的膜样囊性结构，位于最初的囊性结构前方和膀胱的后方，它是第一个囊样结构的镜像。

In the present study, I analyzed the contents of ferromagnetic elemetns and other metal elements in lagenal otoliths of adult homing pigeons with inductively coupled plasma mass spectrometry of high sensitivity(all the contents were normalized to Ca), and compared with those in saccular and utricular otoliths.

为此，本研究中应用高灵敏度和稳定性的电感耦合等离子体质谱法测定成年信鸽瓶状囊耳石样品中铁磁性元素Fe、Co、Ni以及其它金属元素的含量(以Ca作为基准的百分比含量)，并同内耳的球囊和椭圆囊耳石中的这些元素的含量进行比较。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。