囊孢子的

Objective To explore the ageand gender of mice, the optimal dose of the dexamethasone phosphate and the optimal time of immunosuppression in the mice model applied for C.parvum oocyst infectivity and reproduce.

目的 探索适合隐孢子虫卵囊传代和扩增模型小鼠的性别、日龄、免疫抑制时间和免疫抑制剂地塞米松磷酸钠的最佳剂量。

Each embryo were inoculated by a dose of 1×104 pure sporozoites was injected with testing Bt crystal proteins before 24 hours. The varying oocyst inhibition rates of embryos brought about by Bt crystal proteins that were given at various doses were used as a criterion for determining the activity of Bt crystal proteins.

每一鸡胚接种1×104个子孢子，于感染前24h注入苏云金杆菌晶体蛋白，以不同苏云金杆菌晶体蛋白对鸡胚卵囊所产生的抑制率的差异，判定苏云金杆菌的活性。

Monosomic 3, monosomic 5, monosomic 10, trisomic 4 and trisomic 5 as experiment materials, their meiosis behavior of pollen mother cell, pollen characteristics, physiological and biochemical characteristics of anther, section observation of pollen development and the structure of embryo sac were studied, and the effects on the seed set and transmission rate were analyzed.

本文以大白菜同源四倍体小孢子培养产生的且已鉴定为3号单体、5号单体、10号单体、4号初级三体、5号初级三体的非整倍体株系及二倍体株系等为试材，对各材料的结籽率、雄配子传递率进行了统计，并对花粉母细胞减数分裂行为、花粉特性、花粉发育和胚囊结构以及花药生理生化特性等方面进行了观察研究，进而分析了其对大白菜单体n-1雄配子和三体n+1雄配子传递率的影响。

In order to understand the validity of the resistant genes to wheat powdery mildew in Shanxi province, in greenhouse condition, cleistothecia released ascospores were used to infect wheat seedling. The frequencies of virulence genes for 166 isolates of Erysiphe graminis f. sp. tritici from different ecological areas of Shanxi province were tested.

为明确小麦白粉病抗性基因在山西省的有效性，在温室条件下，通过闭囊壳释放子囊孢子侵染麦苗发病，利用28个已知抗病基因品种对分离自山西省不同生态区的166个小麦白粉病菌株进行了毒性基因频率测定。

Antheridia occurred in the cushion on the dorsal and ventral surfaces of gametophytes while archegonia were observed on the ventral surface only. On hermaphroditic gametophytes, antheridia and archegonia were usually intermingled. Both types of gametangia were eusporangiate.

藏精器与藏卵器分别在孢子洒播10及12月后发生；藏精器长在中肋的背面及腹面，藏卵器只在腹面发生；两性配子体的藏精器与藏卵器混生一起，两者均属厚囊蕨型。

Briefly, the new Nested PCR has a bright prospect of application in the microdetection of Cryptosporidium oocysts because of its high specificity and sensitivity.

综上所述，新建立的Nested PCR技术是一种具有较高特异性和敏感性的微量隐孢子虫卵囊检测方法，具有较好的应用前景。

The plasmid pcDNA3.1-3-1E and pcDNA3.1 was respectively used to immunize SPF chicks by injection intra-muscularly in thigh muscle at 14 and 21 days of age.Chicks in each group except that in the unchallenged control group were challenged with 5×104 E.acervulina sporulated oocysts at 28 days post-inoculation,and the immune protective efficacy was observed.

将构建的重组质粒以及空载体质粒分别于14、21日龄分2次经腿部肌肉注射免疫SPF雏鸡，28日龄除非免疫非感染对照组外各组攻击性感染5×104个堆形艾美球虫孢子化卵囊，观察真核表达质粒对球虫感染鸡的免疫保护作用并探讨其免疫保护机理。

To analyze effect of diclazuril and maduramycin on 18S rDNA of Eimeria tenella, the 18S rDNA of sporulated oocyst of the E. tenella anti-diclazuril strain, the E. tenellaanti-maduramycin strain and the E. tenella drug-sensitive strain were cloned and sequenced, and were compared by using DNAStar 5.0 and RNADraw 1.1, respectively.

为探讨抗球虫药物地克珠利和马杜拉霉素对柔嫩艾美球虫18S rDNA的影响，分别对人工诱导的柔嫩艾美球虫地克珠利抗药株、马杜拉霉素抗药株和药物敏感株孢子化卵囊的18S rDNA基因进行了克隆测序，并通过生物信息软件进行对比差异分析。

It"s vegetal mycelia were colorless, smooth, 1.2μm～3.0μm crude, manyconidiogenous cells born on vegetal mycelia, sometimes as many as a dozen,a very small number were single. The base of conidiogenous cell wasenlarged and many of them were bottle-shaped or spherical. The upper ofconidiogenous cell extended into a conidiogenous axis of 1μm wide and 14μm length which was the knee-bending ("Z like bending), there were small pectinae on the axis, conidium located on the small pectina.

营养菌丝无色，光滑，有隔，粗1.0μm～3.0μm，产孢细胞常浓密簇生于菌丝或泡囊上，有时可多达十几个，极少数单生，产孢细胞基部膨大，多为瓶形，近球形，颈部明显延长成粗1μm，最长达14μm的产孢轴，呈膝状弯曲，轴上具小齿突，分生孢子位于小齿突上。

Giemsa staining was used to observe the growth of C.andersoni,and transmission electron microscope was used to observe the developmental stages of C.andersoni.MTT assay was used to evaluate the toxicity of nitazoxanid and ginkgoic acids to HCT-8 cells.C.andersoni parasites growing in HCT-8 cells were treated with different concentrations of NTZ and GAs,and the copies of COWP gene were determined by Real-time PCR.

安氏隐孢子虫卵囊感染人结肠癌(HCT-8)细胞，Real-time PCR技术测定RPMI-1640培养液中分别加入不同浓度胎牛血清(fetal calf serum，FCS)、葡萄糖(glucose，GLU)、维生素C(ascorbic acid，ASC)、叶酸(folic acid，FOLIC)、泛酸钙(calcium pantothenate，CAPAN)和胰岛素(insulin，INSULIN)后虫体的增殖效果。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！