囊孢子的

Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly. Between the 60th and 72th hour, many oocysts emerged. Inoculated by the C. parvum -infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected.

在感染后72 h内，隐孢子虫出现连续发育阶段，包括脱囊、子孢子、裂殖子、裂殖体、滋养体、配子体、合子、薄壁卵囊和厚壁卵囊，在60～72 h内形成卵囊；用感染48 h的细胞培养上清接种于免疫抑制小鼠， 10 d后有隐孢子虫卵囊排出。

The author first reported the social phenomenon and synchronizing development incryptosporidia,the dynamic process of the sporozoites or merozoites aparted from theirparent substances and developed into trophozoites,the culture of C.baileyi in chickenembryo,and the excysting process without both the trypsin and the sodium taurocholatein 37℃ water.The high levels of excystation(60%)oceured at 37℃ water showedthat temprature was very important in cryptosporidia excysting process.

在国内首次观察和描述了薄壁型隐孢子虫卵囊；首次报道隐孢子虫具有群体寄生现象和同步发育特征；首次描述了隐孢子虫的裂殖子或子孢子离开母体和发育为滋养体的动态变化过程；首次应用粪便中纯化的C.baileyi卵囊感染鸡胚成功，并用37℃水脱囊，其脱囊率高达60％，表明温度对隐孢子虫的脱囊具有重要作用，无需胰酶和牛胆酸钠的参与。

The sporulated oocyst holds 2 sporocysts and each sporocyst contains 2 sporozoites,i.e.each oocyst contains a total of 4 sporozoites.

新排出的未孢子化卵囊培养后可观察到孢子化的卵囊，内含2个孢子囊，每个孢子囊内含有2个折光性较强的子孢子。

Lysis solutions from imported and domestic genomic DNA purification kits,and 2% Trition X-100 and 5%guanidinium isothiocyanate were added into the oocysts,followed by different combining forms of freeze-thawing,proteinase K and sonication.Real-time PCR was used to determine the copies of Cryptosporidium oocyst wall protein gene.HCT-8 cells were infected by C.andersoni oocysts.Real-time PCR was used to determine the enhancement effect of fetal calf serum,glucose,ascorbic acid,folic acid,calcium pantothenate and insulin while added into RPMI-1640 medium.

在隐孢子虫卵囊悬液中分别加入进口试剂盒和国产试剂盒的裂解液以及2%Triton X-100和5%异硫氰酸胍，辅以不同组合方式使用的冻融法、蛋白酶K法和声裂法裂解卵囊，通过Real-time PCR法测定裂解产物中隐孢子虫卵囊囊壁蛋白(Cryptosporidium oocyst wall protein，COWP)基因拷贝数。

Microspore Ye as the feather, small sporophyll sac formed by the polymerization, each polymer capsules formed by four small sporangia, small spores with dual airbags, and airbag Pinaceae pollen is similar to large sporophyll from the central axis and arranged in Brachypodium feather composition, Each Yibing has a shell bucket, each bucket containing shell upright ovules 10-12 months.

小孢子叶枝为羽状，小孢子叶由聚合囊组成，每个聚合囊由4个小孢子囊组成，小孢子具双气囊，与松科植物花粉的气囊相似，大孢子叶由中轴及羽状排列的短柄组成，每一柄上有一个壳斗，每个壳斗内有10—12个直立的胚珠。

Soil available N was positively correlated with spore density of Salvia miltiorrhiza and Dioscorea opposite, and hyphal and total percent colonization of Adenophora borealis, while negatively correlated with arbuscular colonization of Aster tataricus; Soil available P exhibited a positive correlation with arbuscular colonization of Salvia miltiorrhiza and vesicular colonization of Dioscorea opposite, while a negative correlation with spore density of Platycodon grandiflorus and vesicular colonization of Atractylodes macrocephala; Spore density of Bupleurum Chinense had a positive affection with soil organic matter; Soil pH was negatively correlated with hyphal and total colonization of Adenophora borealis and spore density of Bupleurum chinense, while positively correlated with spore density of Adenophora borealis.

土壤碱解N与丹参和麻山药的孢子密度、北沙参菌丝定殖率和总定殖率呈显著正相关，与紫苑丛枝定殖率呈显著负相关。土壤速效P与丹参丛枝定殖率、麻山药泡囊定殖率呈显著正相关，与桔梗孢子密度、白术泡囊定殖率呈显著负相关。土壤有机质与北柴胡孢子密度呈显著负相关。土坡pH与北沙参菌丝定殖率和总定殖率、北柴胡孢子密度呈显著负相关，与北沙参孢子密度呈显著正相关。

Spore shells of myxosporidia are synthesized by 2-7 chitin films. There is a polar capsule at the front-end or both ends and the polar filament in capsule. The polar filament can be drawed out from the capsule hole.

粘孢子虫的孢子外壳由2—7片几丁质片合成，前端或两端有极囊，囊内有极丝，可从极囊的孔中射出。

Tenella YL attenuated strain were used to infect chickens perorally, then the abundant sporulated oocysts were collected by saturated saline floating measure. The size of the attenuated oocysts was about 19.8~21.2×15.5~16.6μm and the conformation of the strains were integral. 4 The prepatent period of E.

tenella YL减毒株孢子化卵囊经口服感染鸡，用饱和食盐水漂浮法能够收集得到球虫孢子化卵囊，杂质少，形态完整，减毒株卵囊的大小为19.8~21.2×15.5~16.6μm。

Megaspore mother cells undergo meiosis, which results in the formation of 4 megaspores in a row. The chalazal-most cell develops into an 8-nuclei embryo sac after 3 rounds of mitosis. A mature embryo sac is produced after cellularization and nuclear migration.

结果表明：1大孢子母细胞经过减数分裂形成直线排列的4个大孢子，其中珠孔端的3个大孢子退化，合点端的大孢子进行3次有丝分裂，形成八核胚囊， 8个核迅速细胞化，最终发育为成熟胚囊。

Ninety seven spores or sporocarps of VAMF were wet sieved from the rhizosphere soil of mycorrhized pteridophytes, from which 25 VAMF species which belonged to the genera of Acaulospora, Gigaspora, Glomus, Sclerocystis and Scutellospora respectively were identified, and the taxonomic characters of the 25 species were described. The Glomus fungi were the dominant VAMF in the rhizosphere soil of pteridophytes, they took up 44% in the identified 25 VAMF (11 species belong to Glomus); the fungi in Acaulospora and Sclerocystis in the next place, they took up 24% and 16% respectively in the identified VAMF; the fungi in the other genera took up 16%. In terms of the relationships of pteridophytes and the VAMF isolated from their rhizosphere soil, there was no specifity between the VAMF and their host.

从具有VA菌根的蕨类植物对应的根际土壤中用湿筛法筛取了97份VA菌根真菌的孢子或孢子果，从中鉴定出了分属于无醒囊霉属、巨孢囊霉属、球囊霉属、硬囊霉属和盾孢囊霉属的VA菌根真菌25种，并对这25种真菌的分类学特征进行了描述；球囊霉属真菌是蕨类植物根际土壤中的优势类群，已鉴定的25种真菌中，有11种属球囊霉属真菌，占44%，其次是无梗囊霉属和硬囊霉属真菌，分别占24%和16%，其他属的VA菌根真菌占16%，从分离鉴定的蕨类植物根际土壤中的VA菌根真菌与蕨类植物的关系看，VA菌根真菌与蕨类植物之间无明显的专一性。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！