囊孢子

Based on 28S large subunit ribosomal RNA gene D1D2 fragment sequence analysis, and the difference in the morphology and structure of zoosporangia, most species

本研究以28S核醣体DNA序列D1D2片段分析，并搭配其游动胞囊形态与游动孢子之释放机制研究其类缘关系。

The Actinobacteria are distinguished by producing actinospres: entire cells encysted in thick walls to form resistant spores.

放线菌的特征是能够产生放线菌孢子，它是一种被囊的具有厚壁的完整细胞，具有一定的抵抗能力。

The plasmid pcDNA3.1-3-1E and pcDNA3.1 was respectively used to immunize SPF chicks by injection intra-muscularly in thigh muscle at 14 and 21 days of age.Chicks in each group except that in the unchallenged control group were challenged with 5×104 E.acervulina sporulated oocysts at 28 days post-inoculation,and the immune protective efficacy was observed.

将构建的重组质粒以及空载体质粒分别于14、21日龄分2次经腿部肌肉注射免疫SPF雏鸡，28日龄除非免疫非感染对照组外各组攻击性感染5×104个堆形艾美球虫孢子化卵囊，观察真核表达质粒对球虫感染鸡的免疫保护作用并探讨其免疫保护机理。

To analyze effect of diclazuril and maduramycin on 18S rDNA of Eimeria tenella, the 18S rDNA of sporulated oocyst of the E. tenella anti-diclazuril strain, the E. tenellaanti-maduramycin strain and the E. tenella drug-sensitive strain were cloned and sequenced, and were compared by using DNAStar 5.0 and RNADraw 1.1, respectively.

为探讨抗球虫药物地克珠利和马杜拉霉素对柔嫩艾美球虫18S rDNA的影响，分别对人工诱导的柔嫩艾美球虫地克珠利抗药株、马杜拉霉素抗药株和药物敏感株孢子化卵囊的18S rDNA基因进行了克隆测序，并通过生物信息软件进行对比差异分析。

Ten 10-day-old artificially-reared coccidian-free goslings were inoculated orally with 2.0×105-6.5×105 of sporulated oocysts of Eimeria stigmosa,respectively, and killed at intervals from 48th h to 180th h post-inoculation.

给10只10日龄雏鹅分别经口接种2.0×105~6.5×105个多斑艾美球虫孢子化卵囊，在接种后48~180h分期剖杀，对多斑艾美球虫的生活史和致病性进行了动态观察。

It"s vegetal mycelia were colorless, smooth, 1.2μm～3.0μm crude, manyconidiogenous cells born on vegetal mycelia, sometimes as many as a dozen,a very small number were single. The base of conidiogenous cell wasenlarged and many of them were bottle-shaped or spherical. The upper ofconidiogenous cell extended into a conidiogenous axis of 1μm wide and 14μm length which was the knee-bending ("Z like bending), there were small pectinae on the axis, conidium located on the small pectina.

营养菌丝无色，光滑，有隔，粗1.0μm～3.0μm，产孢细胞常浓密簇生于菌丝或泡囊上，有时可多达十几个，极少数单生，产孢细胞基部膨大，多为瓶形，近球形，颈部明显延长成粗1μm，最长达14μm的产孢轴，呈膝状弯曲，轴上具小齿突，分生孢子位于小齿突上。

The method of freeze-thawing plus proteinase K plus sonication provided the highest copy number of COWP gene.

冻融法+蛋白酶K法+声裂法提纯隐孢子虫卵囊DNA效果最佳，其次为冻融法+声裂法和蛋白酶K法+声裂法，冻融法+蛋白酶K法效果最差。

Giemsa staining was used to observe the growth of C.andersoni,and transmission electron microscope was used to observe the developmental stages of C.andersoni.MTT assay was used to evaluate the toxicity of nitazoxanid and ginkgoic acids to HCT-8 cells.C.andersoni parasites growing in HCT-8 cells were treated with different concentrations of NTZ and GAs,and the copies of COWP gene were determined by Real-time PCR.

安氏隐孢子虫卵囊感染人结肠癌(HCT-8)细胞，Real-time PCR技术测定RPMI-1640培养液中分别加入不同浓度胎牛血清(fetal calf serum，FCS)、葡萄糖(glucose，GLU)、维生素C(ascorbic acid，ASC)、叶酸(folic acid，FOLIC)、泛酸钙(calcium pantothenate，CAPAN)和胰岛素(insulin，INSULIN)后虫体的增殖效果。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！