囊外的

The results showed:(1) the cellule morphous and the disposition in chick embryo of PGCs were coincident no matter stained by PAS or HE staining, and HE staining could disclose the morphologic characteristics more clearly, exactly and completely;(2) genesis of blood island could be observed at the boundary of light and dark region of the extraembryonic blastoderm at about 26 hours;(3) both the blood vessel endothelium cells and free cells of the blood island were differentiated from PGCs. The generating of genuine yolk sac was at about 44-48 hours.

结果表明：①用经典的识别原生殖细胞的PAS染色方法与HE染色所观察到的PGC在鸡胚的分布及细胞形态基本一致，而HE染色较PAS法能更有助于清晰、完整、准确地识别PGC的细胞内部结构特征；②在孵化26小时的鸡胚胚外胚盘的明暗交界处可见血岛的发生，血岛发生的时间比真正的卵黄囊产生时间（大约44-48小时）要早很多；③血岛周围的血管内皮细胞与管腔中游离细胞都来自PGC。

We discovered that the mechanisms of borneol opening BBB involved (1) promoting the tight junction of endothelial cells opening:(2) increasing the quantity of pinocytosis vesicle in endothelial cells and enhance the pinocytosis;(3) inhibiting the activation of P-glycoprotein (P-glycoprotein is an efflux pump of drugs) and increase the permeability of BBB;(4) reducing the expression of intercellular adhesion molecule-1 in brain microvessel endothelial cells;(5) increasing the concentration of Ca〓 in brain microvessel endothelial cells;(6) increasing the activation of eNOS in brain microvessel endothelial cells.

随后，在对冰片开放血脑屏障的机制作进一步的研究时又发现，冰片开放血脑屏障的机制包括以下几个方面：(1)冰片可使血脑屏障内皮细胞间的紧密连接开放；(2)冰片能使内皮细胞内的囊泡数量增加，吞饮功能增强；(3)冰片能抑制P-糖蛋白的活性（P-糖蛋白是一种药物外排泵），而使血脑屏障的通透性增加；(4)冰片能使脑微血管内皮细胞ICAM-1表达量减少；(5)冰片使脑微血管内皮细胞内的Ca〓浓度升高；(6)冰片可升高脑微血管内皮细胞eNOS的活性。

Bioclimatic architecture realizes low energy-consumption natural ventilation: the squama-curtain of TBA TOWER has opening window and special ventilation fan, realizing air ventilation across both sides of the mansion through pressure difference between windward side and leeward side, under the condition of no pressure difference, it can form chimney effect through vacant areas in the mansion, create negative pressure in the internal capsule, import air into device layer and discharge it, realizing the circulation of fresh air, not only saving energy and improving air quality in the room, but also bringing benefits to the health and mind of inhabitants.

生物气候建筑实现低能耗自然通风：TBA TOWER鱼鳞状外幕墙设计有开启扇及专有的通风器，通过建筑迎风面与背风面的压力差实现建筑两面的窗户空气流通；在没有两面压差的情况下，可通过建筑内的挑空区形成烟囱效应，在内囊造成负压，将空气引入设备层排出，实现新鲜空气的流通，既节能、又可改善室内空气品质，利于居者的身心健康

That by the end of November last pregnancy, 34 days in the way of life has done a night's dream, the result is a stomach ache for 20 minutes, when the doctor is afraid of ectopic pregnancy or threatened abortion, let me stay Baotai results, although the play HCG and progesterone of the needle, but that value has been reduced, B-have not seen extrauterine intrauterine pregnancy sac, and later on January 7 (52 days is about the time) see red on the spontaneous abortion, and did not do Qing, because doctors say the stream clean, do not need to do Qinggong, my last time is very short it?

去年11月底那次怀孕，也是在34天的样子，晚上做了性生活的梦，结果也是肚子疼了20分钟，当时医生怕是宫外孕或先兆流产，结果让我住院保胎，虽然打了HCG和孕酮的针，但是那个值一直在降，B超宫内宫外都看不到孕囊，后来1月7日（大概是52天的时候）见红就自然流产了，没有做清宫，因为医生说流干净了，不需要做清宫，我这次和上次间隔时间是不是很短呢？

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

The sac structure projected through the pollen aperture possesses the typical nature of transfer cell, i. e complex lybrinthian wall ingrowth and abundant mitochondria, RER, dicotysomes and ribosomes under the wall. Its outer surface is closely related to the tapetal protoplast. The accumulation of storaged material in pollen start at the area near the apertures. Above restults indicate the pollen apertures may be the"door"from where nutrition flows into pollen in P. hortorum.

绒毡层物质向花粉粒的短途运输明显的表现在花粉的萌发孔区，一方面形成向外突起的囊腔，另一方面在这里具有发达的壁迷宫，而且迷宫下具丰富的线粒体，RER和高尔基体等细胞器，此外花粉内贮藏物的大量积累首先从萌发孔附近开始以及萌发孔处与绒毡层有紧密接触都说明天竺葵花粉萌发孔可能是物质进入花粉的&门户&。

Methods Basolateral amygdala slices of rats were prepared. Field excitatory post-synaptic potentials of BLA were recorded by stimulating the external capsule. Two TBS's were applied to induce LTP in BLA. Each TBS included a brief, high-frequency pulse train (5 pulses at 100 Hz) given at the theta-rhythm (5Hz) for 4 seconds. Experiments compare the effects of different intervals of two TBS's on the expression of LTP in BLA.

制备杏仁核脑片，刺激外囊记录BLA场电位，应用两串θ频率波刺激诱导LTP，每串θ频率波刺激为20个(频率5Hz)短时间高频串脉冲(5个脉冲，频率为100Hz)，通过改变两串θ频率波的刺激间隔，分析不同参数诱导的LTP是否存在差异，并在灌流的人工脑脊液中加入TPK抑制剂genistein，观察其对杏仁核LTP的影响。

Green leaves, like a pin stripes main vein from the leaves has been grown out of the final Linked leaves vein became a long Juanxu, Juanxu the bottom of one of the 10-centimetre-long "bottle", whatever the field through the lips, then there is a bottle phenomenon, This is the lid -- like bottles favorite insect leaf cages, fishing worms Capsule cones shaped, slightly lower expansion, and some like pig cage, so that pitcher plants.

嫩绿的叶子上，一根大头针般粗细的主叶脉从叶片上一直生长出来，最后连着叶片的叶脉成了一根长长的卷须，卷须的底部系着一只十余厘米长的&瓶子&，瓶口的唇向外翻，瓶子上方还有一片小叶，就像瓶子的盖子―――这就是爱吃虫子的叶笼了，捕虫囊圆筒形，下半部稍膨大，有些像猪笼，故称猪笼草。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。