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囊周膜

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Material and MethodsRA Rabbit Model group: 15 early RA rabbits of the same weight and variety Control group:10 normal rabbits of the same weight and varietyMethods of making RA rabbit model:elect 15 normal rabbits of the same weight and variety, dissolve Ovalbumin in 0.9% sodium chloride solution, to make the solution of 20mg/ml concengtration, blend the same quantity of complete Freund′s Adjuvant equably ,inject the mixed solution into endermic tissue of the rabbit′s scapular section, making the rabbits allergic, inject 1 ml of the mixed solution to one rabbit every time, inject 1 ml of the mixed solution in 5 different places of the rabbit′s scapular section, inject the rabbit one time every week,inject 3 weeks continuously,it turned out to be that the rabbits will be allergic, inject Ovalbumin blent with the 0.9% sodium chloride solution into the knee joint cavity of the rabbits in the fourth week, 5 mg Ovalbumin every knee joint cavity,two knees of every rabbit of the 15 rabbits are both injected,the arthrosis diameter and the exterior temperature of the knees will be added obviously in 24 hours,and they will drop gradually,at the time of the 14th or 21th day, the arthrosis diameter and the exterior temperature of the knees will get to the balance time,the incidence rate of RA is 100%.after the RA model succeeds,it is the early time of RA from the first week to the fourth week,after the fourth week,it is the late time of RA, the cartilage of the femoral condyle and the subchondral bone cortices will be changed unrecoverily.

资料与方法RA模型组:早期RA家兔模型15只,品种及体重接近。对照组:正常家兔10只,品种及体重接近。RA家兔模型制作方法:选取15只成年家兔,体重、品种接近,将卵蛋白溶解于生理盐水,配成浓度为20mg/ml的溶液,与等量完全弗氏佐剂混匀,注入家兔肩胛区皮下致敏,每只家兔每次注射1ml,于肩胛区5个不同区域注射,每周一次,连续注射3周而致敏,第4周向膝关节腔注射卵蛋白生理盐水溶液,每只关节腔注射5mg卵蛋白,15只家兔膝关节全部注射,24h内此关节直径和表面温度大幅度上升,以后缓慢下降,至14~21d达到平台期,发病率达100%。造模成功后第1~4周为早期改变,第4周以后出现不可逆的关节软骨及骨破坏。采用高频超声对RA模型组与对照组的膝关节髌上囊液体厚度、滑膜及股骨髁软骨厚度及软骨下骨皮质的回声情况进行对比观察。结果RA组模型组膝关节髌上囊积液及滑膜的厚度明显厚于对照组,其股骨髁软骨的厚度与对照组相比无明显差异,其软骨下骨皮质与对照组相比无明显改变。

The releasing pattern of secretory granules or polypeptide hormones of neurohypophysis involves a whole-releasing pattern together with granular membrane. After released into perivascular space, they enter easily into cerebrospinal fluid via interspace of tissue and epithelial openings rather than into blood circulation through the walls of capillaries, and then into the cerebrospinal fluid.

神经垂体多肽激素分泌颗粒的释放形式,存在一种连同颗粒被膜的整体释放;释放入血管周隙内的多肽激素或分泌颗粒,更易经组织间隙、囊上皮孔进入脑脊液,而非直接透过毛细血管壁进入血液循环再进入脑脊液中。

One view is that the nucleocapsids get tegument in the nucleus, and by budding into perinuclear space, they obtain their envelope from the inner lamella of the nuclear membra...

一种意见认为,核衣壳在细胞核内获得被膜,并通过出芽在内层核膜处获得其囊膜后,进入核周间隙,进一步经与核周间隙相连接的内质网,以囊泡转运的方式经高尔基体到达细胞膜,囊泡膜与细胞膜融合,病毒颗粒释放到细胞外。

Results An obvious increase of SPIR brought about by overextended opening was mainly in the anterior extending tissue of the disc, the bilaminar zone and the subsynovial layer of the articular capsule, and the extent of the reaction became greater with the increased duration of over-opening.

结果 过度张口主要使关节盘前伸部、双板区和关节囊滑膜下层SPIR显著增强,并随过度张口的延长而加重,去除刺激休息两周后,SPIR明显减弱。

Results An obvious increase of SPIR brought about by overextended opening was mainly in the anterior extending tissue of the disc, the bilaminar zone and the subsynovial layer of the articular capsule, and the extent of the reaction became greater with the increased duration of over-opening. The SPIR decreased obviously two weeks after removing the nocuous stimulation.

结果 过度张口主要使关节盘前伸部、双板区和关节囊滑膜下层 SPIR 显著增强,并随过度张口的延长而加重,去除刺激休息两周后, SPIR 明显减弱。

The diffusive XGPN shows cystic-solid multi-occupying with the whole kidney involved and kidney renal pelvis and calices enlarged. The lesions were extensive with unclear border which made the perirenal space dim renal fasciae thicken and with the abscess formation of psoas and abdominal wall. The localized XGPN shows mass in the renal parenchyma with low density clear border after enhancement and rim enhancement.

弥漫型累及全肾,肾影增大,肾盂肾盏扩大积液,肾实质幕粥发囊实性占位,病变分界不清,向肾外扩展,肾周间隙模糊,肾筋膜增厚并累及腰大肌,腹壁脓肿形成;局灶型表现为肾实质内局限性占位,病灶区呈略低密度灶,增强后边界清晰,病灶内见环形强化,而略低密度灶者强化较明显,但仍低于肾实质。

The results by invert embedding TEM showed that higher invasive CCL229cells had vast of ER,which distributed throughout cytosol especially largeamount of vesicle-like and flatten cisternal rER in pseudopodia or cell processesand membranous flow-like structure from perinuclear region to pseudopodia,and less Golgi complex.

倒置包埋法常规透射电镜对人大肠癌细胞的超微结构观察结果显示,高侵袭力的CCL229细胞中内质网数量较多并呈全细胞分布,尤其在细胞伸出的众多伪足中含有丰富的小泡状和扁平囊样粗面ER结构,而且观察到由小泡形成的&膜流样&结构从核周一直深入到伪足中,但细胞中高尔基器少见。

4Histological examination of the capsular bags: It could be seen in group 4 that a small amount of LECs at the capsular equator proliferated and migrated to the posterior capsule, forming a thin soemmering ring in the periphery of the capsular bags, forming thin fibromembrane which embodied monolay metaplastic fibroblast-like cells in the center of the capsular bags, or with no cells or cortical material in the center of the capsular bags.

囊袋组织学检查:HSV-TK+GCV组可见少量赤道部晶状体上皮细胞(lens epithelial cells, LECs)增殖并向后囊膜迁移,在囊袋外周部形成稀薄的soemmering环,在中央部无细胞和皮质,或仅形成单细胞层纤维膜。

During the wall thickening, the number of Golgi bodies increased apparently and cytoplasm was filled with Golgi vesicles. Polylamellate structure of the secondary wall appeard, Along with the further development of tracheary elements, the nucleous disappeared and organelles were decreased, while cortical microtubules were arranged neatly against the inner side of plasmalemma.

初生壁与质膜之间有间隔较规律的次生壁突起;中期,线粒体和高尔基体的数量明显增加,细胞质中几乎充满高尔基体及其囊泡,次生壁中微纤丝已成有序排列;晚期,细胞核消失,导管中央形成空腔,细胞器迅速减少,仅观察到少量高尔基体和线粒体,有大量周质微管沿细胞长轴分布,且在次生壁的周围有大量小泡附着,次生壁的分层结构清晰可见。

D. N big mouse model was successfully made by adopting single side kidney cutting with induction, then by 24 week continuos observation on the change of various values of kidney and function, through testing of immunity florescence, electro-scope and optical scope, what we can see from model group is that typical kidney small cell got hardened by knot, and discarded, and dead, also small tube withered, small cell of on base film got thick, cell capsule wall was fibred, and base film got double layered.

实验研究采用单侧肾切除加以链脲佐菌素诱导,成功地复制出糖尿病肾病大鼠模型,对其肾脏结构和功能的改变各项指标,连续进行24周动态观察,并进行免疫荧光、电镜、光镜检测,结果表明:糖尿病肾病模型组可见典型的肾小球节段硬化、废弃、坏死,有大量蛋白管型,肾小球管糖元空泡沉积及脂肪滴,肾小管萎缩,肾小球基底膜明显增厚,球囊壁纤维化,基底膜双层化,有一例结核性肉芽组织及钙化等病理变化。

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