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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在桑葚胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在桑葚胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

One view is that the nucleocapsids get tegument in the nucleus, and by budding into perinuclear space, they obtain their envelope from the inner lamella of the nuclear membra...

一种意见认为,核衣壳在细胞核内获得被膜,并通过出芽在内层核膜处获得其囊膜后,进入核周间隙,进一步经与核周间隙相连接的内质网,以囊泡转运的方式经高尔基体到达细胞膜,囊泡膜与细胞膜融合,病毒颗粒释放到细胞外。

Plants involved in this association are most of the terricolous plants. The fungi related are classified in six genera of order Glomales, they are Glomus, Sclerocystis, Acaulospora, Entrophospora, Gigaspora and Scutellospora.

参与丛枝菌根形成的植物是大多数的陆生植物,参与丛枝菌根形成的真菌是隶属于球囊霉目6个属的真菌,即:球囊霉属、硬囊霉属、无梗囊霉属、内养囊霉属、巨孢囊霉属和盾巨孢囊霉属,它们共同的典型特征是在宿主根皮层细胞中能够形成树状的分支,即丛枝。

Results Among all 25 cases of lymphangioma,Within 20 cases of cystic lymphangioma,single cyst was showed in 14 cases and multilocular cyst was showed in 6 cases in plain scan,and little enhancement of capsule wall and septal in 8 cases in enhancement scanning. Cystic-solid mass was showed in 4 cases of cavernoma lymphangioma in plain scan,and solid part of mass was enhanced obviously in enhancement scanning.1 capillary ymphangioma showed the skin and hypodermic thicking,and part of area showed little beehive-like feature. Enhancement scanning showed slight irregularity enhancement.

结果 25例淋巴管瘤中20例囊性淋巴管瘤,CT平扫14例表现为单一囊肿,6例表现为多房性囊肿,囊腔内见有分隔;增强扫描8例出现囊壁或间隔的轻微强化。4例海绵状淋巴管瘤平扫均表现为囊实性肿块,增强检查实性部分有明显分期增强;1例毛细管型淋巴管瘤表现为局部皮肤及皮下组织的增厚,部分区域见有小蜂房样表现,增强扫描见有轻度不规则强化。

After selective extraction, the cyst wall of two kind of resting cysts displayed a filament network. In the resting cysts of Tachysoma pellionella and Euplotes encysticus, the filament was located in mesocyst and endocyst respectively. On the other hand, a special filament scaffold region was observed below the cyst wall in two kind of cysts. Compared with the true IF which was located in central part of cyst, the filament of this region was net as well as uniform, and no any cytoplasm residue can be seen in this region. In the resting cyst of Euplotes encysticus, a filamental boundry which partitioned the special filament scaffold region and the true IF region was observed.

两类休眠包囊的包囊壁经生化分级抽提后均显示纤维结构,急纤虫休眠包囊中纤维定位于中间层,包囊游仆虫休眠包囊中纤维定位于内层壁;其次,在两类休眠包囊的包囊壁下,相当于细胞质表质层的位置,均有一个特殊的纤维网络区,与细胞质深层的网络区相比,此部分的纤维纯净、均匀,未见附着细胞器及细胞质残留物,在包囊游仆虫休眠包囊中此纤维网络区与真正的细胞质纤维网络之间由纤维构成的界膜隔开。

The results were expressed in mean±1SD. Pearson X~2 test and One-way ANOVA test were used. The data analyzed using the SPSS (version 11.5). Results: The sensitivity, specificity, positive and negative value of US for the LPEH model on the children cadaver were 88%, 84%, 79%, 91%, respectively. With regard to the thickness of femora head cartilage, the thickness of the anterior layer or posterior layer, there were no significant differences among three groups. However, the anterior layer was thicker than the posterior layer in three groups. The fluid in hip joint was detected in all of 21 symptomatic hips, which was clear commonly (90%) in early procedure. The amount of fluid in anterior recess showed a positive correlation with age (p .05). No fluid was detected in the asymptomatic and normal hips (2mm). The mean maximum width of inferomedial recess was significantly larger than that of anterior recess (12.50±4.04mm vs.4.35±0.8mm, p 0.05) in the symptomatic hip joints. The echogenic entrapped labral plicaes were demonstrated in the inferomedial recess in all of 21 children with LPEH, whose length and width ranged from 5.3mm-25.0mm (mean,15.6±5.6mm) and from 4.0mm-17.0mm (mean,8.9±7.8mm).

结果1,尸体LPEH髋关节模型的超声诊断敏感性、特异性分别为88%、84%,阳性预测值、阴性预测值分别为79%、91%。2,21例患儿的LPEH患髋(21侧)、健髋(21侧),以及21例正常儿童健髋(42侧)的超声检查显示:髋关节周围软组织及股骨头无形态结构差别;股骨头软骨厚度无统计学差异(3.5±0.5mm vs.3.6±0.4mm vs.3.6±0.5mm,p>0.05);关节囊前层及后层厚度无统计学差异(前层厚度2.79±0.74 mm vs.2.56±0.40mm vs.2.56±0.72mm;后层厚度2.70±0.82mm vs.2.48±0.54mm vs.2.44±0.58mm,p>0.05),但LPEH患髋关节囊前、后层均较后二组有增厚趋势。3,LPEH患髋均存在关节腔内积液,且早期较为清晰;积液以髋关节内下间隙明显,内下间隙较前间隙明显增宽(12.50±4.04mm vs.4.35±0.8mm,p<0.05),其内见嵌顿滑膜唇皱襞呈稍强回声的占位性团块,长约15.6±5.6mm,宽约8.9±7.8mm,90.5%(19/21)嵌顿皱襞内未见血流信号。4,所有LPEH患髋治疗后超声复诊均显示正常。

MR may obviously demonstrate the entire image if hepatobiliary cystadenoma, the mural nodus form and corpora mammillaria solid lesions suggest canceration. Conventional MR sequences combine with dynamic enhancement and MRCP possese fairly diagnostic value to hepatobiliaty cystadenoma.

MR能明确显示肝内胆管囊腺瘤的整体影像的特征性表现,若囊壁上有结节或乳头状肿块提示局部有癌变,MR常规扫描结合动态增强及MRCP成像对肝内胆管囊腺瘤具有很高的诊断价值。

There are two ways to produce nuclear vacuoles. One is that the outer membrane of the nuclear envelope distends towards the cytoplasm to form nuclear vacuoles; and the other is the double-layered nuclear envelope derives vacuoles by depressing inwards.

核膜囊泡有两种发生方式:1核膜的外膜向细胞质一侧膨胀产生囊泡,囊泡进入细胞质;2核膜向核内凹陷形成囊泡,囊泡进入细胞核。

There was no typical Golgi complex in oogonia, but there were some Golgi vesicles. At the early stage of vitellogenesis, Golgi complex was abundant whose saccules increased to more than ten layers. At the middle stage of vitellogenesis, Golgi complex grew more in numbers and got together, so its secreting quickened. Moreover, it formed a number of Golgi vesicles, which were filled with granule substances that changed into yolk granule finally.

卵原细胞内无典型的高尔基复合体,但具高尔基囊泡;卵黄发生早期,高尔基复合体发达,膜囊数达10多层;卵黄发生中期,高尔基复合体分泌活动加快,产生大量的大小囊泡,囊泡内充满颗粒状物质,最后演变成卵黄颗粒。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。