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(1) In common cavity malformation, in addition to the abnormal bony labyrinth such as the cochlea, vestibule and lateral semicircular canal, the inner ear perceptive organ such as the saccule, utricle and scala media were also abnormal.(2) Though we could not identify the basal membrane and sensory epithelia under micro-endoscopy, the utricle and saccule could be clearly identified.(3) During cochlear implantation in common cavity malformation, electrode insertion should be monitored under micro-endoscopy, the electrodes should be put close to the anterior wall of the cavity, otherwise the vestibular organ may be damaged.

(1)共同腔畸形极重度聋患者不仅前庭、外半规管和耳蜗的骨性结构形态发生了变化,而且腔内耳蜗中阶与前庭的球囊、椭圆囊的膜迷路结构形态也发生了变化;(2)微窥镜下分辨不出基底膜或听神经细胞的形态结构,可见畸形前庭的球囊、椭圆囊的囊性结构;(3)进行共同腔畸形人工耳蜗植入手术时,应在耳蜗内镜的监视下,将电极摆放到准确位置,尽可能贴近共同腔的前壁,不应损伤前庭器官。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果:1、哇巴因作用于晶状体钠泵后,光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙,电镜下全层细胞空泡化、线粒体肿胀出现髓样结构,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后,光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离,电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后,光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整,电镜下细胞器丰富、细胞功能旺盛,RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后,光镜下晶状体质膜完整、细胞排列紧密规整,电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝,RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后,光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化,电镜下囊侧膜内有高电子密度带、细胞核仁丰富,RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后,光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏,电镜下质膜变性、线粒体肿胀,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

In the four placebo-controlled studies evaluating radiofrequency denervation outcomes in patients with "confirmed"l-z joint pain, the only study that screened patients with MBB demonstrated positive outcomes,157versus only one of three that used diagnostic intraarticularin jections.158–160 Ultrasound-guided medial branchand intraarticular blocks have also been demonstrated to provide comparable accuracy to fluoroscopically guided injections, although they may be less likely to detect low-volume intravascular uptake and are less accurate in obese patients.161–164 Because MBB are technically easier to perform than intraarticular injections and involve anesthetization of the nerves to be lesioned, it seems more logical to use these blocks as a prognostic tool before radiofrequency denervation.

在四个设有安慰剂对照的对于&确诊&小关节疼痛的患者行射频神经消融后的结果的研究中,唯一一个用MBB筛选研究对象的实验表现出阳性结果,而其他3个用关节内注射筛选的实验中只有1个表现出阳性。超声介导下的内侧支关节囊内阻滞被证实较透视介导更精确,虽然其血管内吸收剂量较低且在肥胖的患者中精确性较差。由于MBB较之关节内注射技术上更简单,且能对受损神经进行麻醉,利用这种阻滞作为神经射频消融前的诊断方法较为合理。

Phaeocystis cells are separated experimentally form the water samples collected in Bohai Phaeocystis blooms in 2005.Plate and dilution was used to separate and culture the Phaeocystis cells from the Bohai water samples; Its ecological properties of growth was studied in this research; The break effect of ultrasonic and freezing method was compared in the breaking treatment of Gymnodinium spp. cells of different periods.

该研究选用平板法和稀释法对渤海棕囊藻赤潮期间水样中的微藻进行了分离培养实验;系统研究了裸甲藻在实验室条件下的生态学特征;考察了超声波法和冻融法对不同生长时期裸甲藻细胞的破碎效果;运用高效液相色谱对裸甲藻和链状亚历山大藻细胞提取物进行了麻痹性贝毒的分析检测。

Because of the absence of a limiting hernial sac, mechanical SBO usually occurs in cases of trans mesenteric hernia (Fig 8), and it is impossible to differentiate closed-loop obstructions caused by herniation through the mesenteric defect from those caused

由于后者没有局限性的疝囊,机械性SBO通常发生在肠系膜疝的病例中(图8),而且不能区分经肠系膜缺损疝导致的闭合性肠梗阻与粘连带下的肠脱垂导致的闭合性肠梗阻。

There hae been a few reported cases of bursal osteochondromatosis inoling the retrocalcaneal, subacromial, and subcoracoid bursae2,10,11; howeer, infrapatellar bursal osteochondromatosis has neer been reported in the English-language literature, to our knowledge.

已报道的粘液囊骨软骨瘤病病例包括跟骨后、肩峰下及喙突下粘液囊的骨软骨瘤病;然而,据我们所知,髌骨下粘液囊骨软骨瘤病的报道却从未在英文文献中见到过。

Objective: The purposes of the present study are as follows: 1 To study the effect of direct contact with VSMC on transdifferentiation of MSC; 2 To construct MSC of Dual-stable expression of AT2R gene medicated by tetracycline regulatable system.; 3 To investigate the effects of AT2R gene medicated by tetracycline regulatable system on neointimal hyperplasia in rat carotid arteries after balloon angioplasty.

本实验体外观察MSC与VSMC在直接接触培养下的分化情况,并以MSC为载体,通过体外细胞基因转染及筛选,获得双重稳定受到强力霉素调控表达AT2R基因的MSC,将此受到调控表达的AT2R基因导入大鼠颈动脉球囊损伤动物模型中,观察MSC细胞移植实现AT2R基因在体可调控表达在新生内膜中形成的作用及潜在意义。

Effects of concentration of atrazine on growth of Scenedesmus quadricauda and Microcystis aeruginosa cultured in MA medium were studied by determining growth rate and content of chlorophyll a in laboratory.

在实验室内利用MA培养液培养,通过测定藻生长量和叶绿素a含量,研究不同浓度下的阿特拉津对铜绿微囊藻和四尾栅藻生长的影响,并以藻细胞数表示的最大比生长率为指标,评价2种藻对阿特拉津的敏感性。

Results:In this group there were 5 mucinous cystadenoma; 4 serous cystadenoma; 4 teratoma; 2 lager ovarian cyst; 4 subserous leiomyoma of uterine. The massive cystic-solid tumor with irregular cystic wall was seen in mucinous cystadenoma and serous cystadenoma; Teratoma appeared non homogenous density with fat density mass; Subserous leiomyoma of uterine showed large cystic tumor with clear edge, and the size of uterine was normal.

结果:5例卵巢浆液性囊腺瘤,4例黏液性囊腺瘤,CT表现为腹盆腔内巨大囊性或囊实性肿瘤,囊壁厚薄不均;4例卵巢畸胎瘤,CT表现为盆腔巨大肿瘤内部密度不均,可见脂肪密度影;卵巢巨大囊肿2例,CT表现为腹、盆内巨大单囊状肿物;4例浆膜下子宫肌瘤,CT表现为盆腔内巨大囊性肿物,边界清晰,子宫大小正常;1例阔韧带平滑肌瘤,CT表现为密度不均匀的实性肿物,增强扫描强化不均。

To elucidate the biological structure basis of transgenic rice plants expressing maize PEPC gene with high photosynthesis efficiency, PEPC transgenic rice and its wild type with at 3 leaves seedling stage were treated under 1000 μmol/(m^2s) light intensity for 100 min, respectively, and those under 200 μmol/(m^2s) light as the control. The structure of mesophyll cells, bundle sheath and thylakoids of the second blade were observed with a transmission electron microscope.

为了阐明转玉米PEPC基因水稻高光效的结构基础,以转玉米PEPC基因水稻和未转基因原种为试验材料,在水稻的三叶期,施以100min,1000μmol/的高光强处理,以200μmol下的水稻为对照,运用透射电镜观察叶肉细胞、维管束鞘以及叶绿体内类囊体片层结构。

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