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Methods:9 patients including 8 male and 1 female were included in this study.2 patients suffered from both side of ANFH,5 were righe side and 2 were left side.X-ray,CT and MRI were performed before the surgery.4 hips were classified as ARCO stage 1,7 hips were stage 2.All the patients were treated as follows:the area of NFH and cystis degeneration were detected and bouche decompression was done through X-ray with C-arm,and the necrotic bone was cleared for the pathologic diagnosis under the monitoring of arthroscopy.And then the non-cell based tissue engineering bone, which was the complex of autologous red bone marrow and osteoinductice absorbing material containing BMP,was transplanted into the body.

本组9例,男性8例,女性1例;双侧股骨头坏死2例,右单侧股骨头坏死5例,左单侧股骨头坏死2例;术前均行X线摄片、CT、MRI 检查,ARCO分期:Ⅰ期4髋,Ⅱ期7髋;C型臂X线透视下行股骨头坏死区和囊性变区定位、髓芯减压,关节镜监视下,刮除坏死骨送病理;植入由自体红骨髓与含BMP的骨诱导活性材料复合成的非细胞型组织工程骨,并用空心钛钉支撑股骨头软骨下骨质。

In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期,高度表达的合子转录本仍在所有的胚盘细胞中,但至90%下包期时,pou2的表达向胚胎背部的正中线汇聚,集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时,pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时,转录本消失,这与RT-PCR结果高度一致。

Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed from blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells.

我们用RT-PCR和整体原位杂交的方法研究了银鲫pou2基因在胚胎发育过程中的时空表达图式RT-PCR结果显示,银鲫pou2基因有母源转录本,其合子基因在高囊胚期强烈表达,在50%下包期和90%下包期也有高量的转录本,但在100%下包期表达量急剧降低,至体节期时已经完全检测不到其转录本胚胎整体原位杂交结果显示其母源转录本在所有的胚盘细胞中。

Results Eleven cases of TBMN in children were showed as isolated hematuria, glomerule with normal appearance or slight change and characterized by diffuse thin basement membrane in ultrastructural observation( the thickness of GBM< 200 nm, and dense-layer< 100 nm).

结果:11例小儿TBMN临床主要表现为单纯血尿,无其它明显的阳性体征,光镜下肾小球无明显改变或轻微异常,未见蛋白管型,包曼囊内及肾小管腔内可见渗出的红细胞,电镜下GBM广泛变薄,平均厚度<200 nm,致密层厚度<100 nm。

Puncture into Attain long sheath of CS through left infraclavicular vein, guided by CS pipe, take sheath into CS, make reverse contrast of heart vein with Attain saccule contrast pipe, put the left ventricle electric pole into the vein through sheath, then bring it into right ventricle apex and right cardiac ear through the electric pole of right ventricle and atrium, connect it with 3-cavity pulse generator, which is buried under left chest skin.

方法]经左锁骨下静脉穿刺送入Attain冠状静脉窦长鞘,在CS导管的导引下,将鞘送人CS,用Attain球囊造影导管进行心脏静脉逆行造影,将左心室电极经长鞘放入选择的静脉,送人右心室及右心房电极至右室心尖和右心耳。与三腔脉冲发生器连接,脉冲发生器埋藏于左上胸皮下。

Take out the daughter cyst under asepsis condition from the liver of the sheep which infects the echinococcosis under natural condition, cut to pieces, remove the cyst skin, flush with 0.9% asepsis physiological saline and precipitate three times, make the HE dyeing counting, make the suspension which is 1200 scolex/0.1ml, then vaccinate directly nearby spinal column, under the hind leg periosteum and in the medullary cavity of the leg bone of the health grown-up meriones meridianus to prepare the animal infection model, then carry on the medicine and the surgery treatment to the model.

从自然感染细粒棘球蚴包虫病的羊肝脏无菌条件下取出子囊,剪碎,去除囊皮,用0.9%无菌生理盐水冲洗、沉淀、反复三次,HE染色计数,制成1200个头节/0.1ml的混悬液直接接种到健康成年子午沙鼠脊柱旁、后腿骨膜下、后腿骨髓腔内制备动物感染模型,然后对模型进行药物和手术治疗。

The CNR of fat and muscle belly on T_1WI was higher than on PDWI(p = 0.000).Conclusions:1、Routine MRI can demonstrate the dependency structure of shoulder clearly.The distance of subcoracoid was 8.84±2.97mm.The distance of sub-acromion was 5.23±1.05mm. 2、The 3D-fiesta imaging with muti-angle reconstruction is better in showing the coracohumeral ligament,coracoacromial ligament,coracoclavicular ligament, capsulolabral complex and the bone of shoulder,combing with oblique coronal and oblique sagittal imaging of the fat-suppression sequence,the diseases of shoulder can be diagnosed.

1、常规MRI能够较清楚显示肩关节相关结构,喙突下距离8.84±2.97mm,肩峰下距离5.23±1.05mm.2、3D-fiesta序列图像,应用重建功能行任意角度重建,可更好地显示喙锁韧带、喙肩韧带、喙肱韧带、盂唇-关节囊复合体及肩关节骨骼,结合斜冠位、斜矢状位的压脂序列,即可达到对肩关节疾病进行诊断的目的。

objectiveto explore the drain way to tear with inferior lacrimal passage ruined critically.methodsthe special silicon tube whose bore 1mm and diameter 2mm was inserted between conjunctival sac and nasal vestibule,the tube was placed under the soft tissue of bucco-nasal groove and in the surface of the protrusion of upper jaw bone.resultsthere were 32 patients who undergone tube above five years.one patient of them was pulled out the tube because of papilloma hyperplasia,the other patient was pulled out the tube with the tube slide below,and the rest 30 patients kept the drain well and co

目的探讨下泪道破坏严重时泪液引流途径。方法在结膜囊-鼻前庭间安置一个内径1mm、外径2mm的特制硅胶导管,此管置于鼻颊沟软组织下,上颌骨前突表面。结果治疗32例,观察5年以上,其中1例因结膜乳头状瘤增生而拔管,1例因导管下滑而拔管,30例引流良好,病人亦能耐受。本文就引流机制、并发症、机体耐受问题和操作进行了详细地讨论。结论泪道外插管是下泪道严重损害时泪液引流的一种较好的解决方法。皮下;结膜鼻腔造瘘术;硅胶导管

If they say,"come along with us; let's lie in wait for someone's blood,let's waylay some harmless soul; let's swallow them alive, like the grave, and whole, like those who go down to the pit; we will get all sorts of valuable things and fill our houses with plunder; throw in your lot with us, and we will share a common purse"...

他们若说,你与我们同去,我们要埋伏流人之血,抢劫无辜的人;我们好像坟墓,把他们活活吞下,让他们全部象掉下深坑;我们必得各样宝物,将所掠来的装满房屋,你与我们大家同分,我们共用一个宝囊。

Methods Under local anesthesia,five cases of vertebrobasilar aneurysms were performed ballons occlusion vertebral artery at the end of PICA which is close to heart after a successful ballon Mata's test.

局麻下股动脉插管,在球囊闭塞试验成功后,闭塞小脑后下开口近端的椎动脉。

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