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High-density material lying at the circumference of a proacrosomal vesicle was arched with a gap in Solen grandis, but round in Solen strictus. From this gap a proacrosomal vesicle of Solen grandis began to invaginate and deform while it moved to the nuclear front. However a proacrosomal vesicle of Solen strictus did not deform until it reached the nuclear front.

大竹蛏精细胞前顶体的高电子密度物质分布在周缘呈一个带缺口的弧形而不是圆形,而长竹蛏前顶体周缘的高电子密度物质呈一圆形分布;大竹蛏的前顶体是先内凹变形,然后一边变形一边移动,而长竹蛏的前顶体是在到达核前端后才开始变形。

Objective To study the change of intracellular free Ca2+ in the oocyst when it melanized and to find out the relationship between the melanized oocyst and its intracellular level of free Ca2+ in a Plasmodium-refractory strain of Anopheles dirus.

目的研究大劣按蚊蚊胃的约氏疟原虫卵黑化时卵内Ca~(2+)的变化,探讨疟原虫感染不易感蚊种时卵黑化与内Ca~(2+)的相互关系。

Results In the aspect of oocyst intensity discharged from mice, femal BABL/C mice were more than the male, the 50 day-old mice(26 g~28 g) were more than the 25 and 75 day-old, mice in 15 mg/L group were more than those in the and mice infected after immunosuppressed for 3 days were more than those infected after immunosuppressed for –6,-3,0,6,9 days.

结果 雌性小鼠排卵强度均较同日龄雄性小鼠大,而不同日龄小鼠中又以50日龄(26 g~28g)小鼠排卵量最多;各剂量组小鼠中,15 mg/L组排卵强度较其他各组大,10 mg/L组小鼠次之;免疫抑制时间各组中,免疫抑制3天后感染的小鼠排卵强度较其他组大。

The mean value of Ca2 + in the mature oocysts was (137.15 ±7.02) nmol/L but was (18.44± 1.75) nmol/L in melanized oocysts with Ca2+ sedimentation in the wall of oocyst.

约氏疟原虫成熟卵内Ca~(2+)为(137.15±7.02)nmol/L,完全黑化卵为(18.44±1.75)nmol/L,并在壁出现明显的钙沉着。

Firstly,the presence and location of AAT in amphioxus was studied by Westernblotting and immunohistochemical methods.The results first revealed that AATpresent in amphioxus,a cephalochordate.Also it was demonstrated that AATdistributed in cellular plasma in hepatic diverticulum,the pouch that protrudesforward as an outpocketing of the digestive tube and extends along the right side ofthe posterior part of the pharynx,which has long been considered to be the precursorof vertebrate liver.In vertebrate,AAT is primary synthesized in liver.

我们首先利用免疫组化和Western blotting方法,研究了AAT在文昌鱼的组织表达和定位,首次在头索动物文昌鱼中证明了AAT的存在,结果显示,AAT定位于文昌鱼肝盲的细胞浆内,从AAT在文昌鱼中的合成部位来看,在功能上文昌鱼的肝盲等同于脊椎动物肝脏,肝盲是在发育分化过程中消化道组织向右前方突出沿着咽后部向前扩展和延伸形成的一个袋状结构。

Lysis solutions from imported and domestic genomic DNA purification kits,and 2% Trition X-100 and 5%guanidinium isothiocyanate were added into the oocysts,followed by different combining forms of freeze-thawing,proteinase K and sonication.Real-time PCR was used to determine the copies of Cryptosporidium oocyst wall protein gene.HCT-8 cells were infected by C.andersoni oocysts.Real-time PCR was used to determine the enhancement effect of fetal calf serum,glucose,ascorbic acid,folic acid,calcium pantothenate and insulin while added into RPMI-1640 medium.

在隐孢子虫卵悬液中分别加入进口试剂盒和国产试剂盒的裂解液以及2%Triton X-100和5%异硫氰酸胍,辅以不同组合方式使用的冻融法、蛋白酶K法和声裂法裂解卵,通过Real-time PCR法测定裂解产物中隐孢子虫卵壁蛋白(Cryptosporidium oocyst wall protein,COWP)基因拷贝数。

Aeruginosa increased with the increased initial P concentration in the media;② Under the constant N concentration, the phosphorus uptake of M. aeruginosa was correlated positively with the initial P concentration;③ Under the constant P concentration, the accumulation of phosphorus by M. aeruginosa was correlated negatively with the initial N concentration, but the trend was not obvious and the effect of initial N concentration on the uptake P quantity became ruleless when the initial N concentration reached a higher level (21 mg/L).

结果显示,①氮磷比不变时,随着起始磷浓度的增加,铜绿微藻对正磷酸盐蓄积量也逐渐增加,成明显正相关;②氮浓度不变时,铜绿微藻对正磷酸盐的蓄积效果与磷浓度正相关;③而同一磷浓度下,铜绿微藻对正磷酸盐的蓄积效果与氮浓度负相关,但氮浓度超过21 mg/L时,氮浓度对于铜绿微藻蓄积磷的效果影响变得不规则。

The DSC analysis results indicated, the characteristic peaks of individual materials disappeared and there was a new characteristic peak at 94. 28℃ in the DSC thermogram of blank alginate-chitosan microcapsules, which meant there was electrostatic interaction between materials consisting the microcapsules.

DSC分析结果显示,在空白海藻酸钠-壳聚糖微的谱图中,组成微的单组分材料的特征峰均消失,于94.28℃处出现新的特征峰,说明微材之间发生了静电相互作用而成型。

The tumors ranged from 7 to 26 cm (mean 13.2 cm). The gross appearance was variable: unilocular cyst with a thin wall (4 cases), predominantly cystic with papillary areas or nodule (8 cases), multiloculated cyst with or without nodules (1 case each), and predominantly solid with cystic areas (4 cases).

肿瘤大小7至26cm(平均13.2cm),大体外观各异:薄壁的单房腔(4例)、腔内含乳头或结节(8例)、多房性腔内含或不含结节(各1例)、主体实性伴腔(4例)。

Multilocular cystadenomas have the typical MRI manifestations and MRI is valuable, whereas unilocular cystadenomas, having similar MRI findings with some other benign cystic lesion, are lack of enough features in MRI. However, thick irregular wall or septa in MRI might be helpful in differential diagnosis.

多房性腺瘤具有较典型的MRI表现,诊断不难;单房性腺瘤缺乏特异表现,壁强化是与其他性病变鉴别诊断的重要徵象,MRI检查对诊断有重要作用。

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