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Methods The contents of ferromagnetic elements and other metal elements in lagenal otoliths of adult homing pigeons were precisely analyzed with inductively coupled plasma mass spectrometry of high sensitivity, and then they were compared with those in saccular and utricular otoliths (all the contents were normalized to Ca).

应用具有高灵敏度和稳定性的电感耦合等离子体质谱法测定成年信鸽瓶状耳石样品中磁性元素Fe、Co、Ni以及其它金属元素的含量(以Ca含量作为基准的百分比含量)并同内耳的球和椭圆耳石中的这些元素的含量进行比较。

Methods Neomycin was used to destroy guinea pig utricular hair cells in vitro.The culture in experimental groups was supplemented with bFGF.The culture in control group was not supplemented with bFGF.All utricles were observed by scanning electronic microscope,and the number of survival utricular hair cells was accounted.

采用离体的豚鼠椭圆培养法,对照组为普通前庭培养液培养,实验组按培养液中含和不含bFGF分组,用新霉素造成各实验组培养的椭圆毛细胞损伤,各组椭圆均行扫描电镜检查并行毛细胞计数。

AQP3, 7, 8 distribute in a similar manner which is surrounding the membranous labyrihth, including Cortis organ, inner and outer spiral sulcus, stria vascularis, fibrocytes of the spiral ligament and the spiral limbus, saccular and utricular wall, endolymphatic sac and spiral ganglion.

AQP3,7,8三者的分布类似,在螺旋神经节和包绕膜迷路的组织中表达,包括Corti's器、内外螺旋沟、血管纹、螺旋韧带纤维细胞、螺旋缘、椭圆壁、球壁、内淋巴等。

Presence of pseudoexfoliation syndrome, nuclear hardness, pupil size, phaco time, effective phaco time, systemic diseases, perioperative complications capsulorhexis rupture, zonular dialysis, posterior capsule rupture .

假性膜剥脱综合症,核硬度,瞳孔大小,超声时间,实际超声时间,系统性疾病,术中并发症(膜撕裂,悬韧带断裂,后破裂伴玻璃体丢失),IOL植入位置和术后并发症作为膜形成的危险因素分析。

Results solid mass 78 cases, Cystic lesion 4 cases , Cystic and Solid lesion 31 cases. The lesion have clear smooth and intact capsule. The meningeal tail sign and Cranial Cortex burking sign are especial features, Cystic lesions qualitalive diagnosis is difficult and making diagnosis must be all-around ananyse.

结果: 实质性肿块78例,性4例,实性31例,病变边界清楚,外壁光滑,有较完整包膜,脑膜&尾&征和脑外皮质&扣压&征为其特征表现,性占位定性诊断有一定困难,需全面分析才能作出诊断。

The LBL self-assembly process was monitored by flow cytometry and zeta potential analyzer. Scanning electron microscope, dynamic light scattering, and flow cytometry were used to characterize microcapsules The microcapsules were 1.14 μm in size and stable in lyophilizing.

在微的制备过程中,采用流式细胞仪和Zeta电位仪对层层自组装进行监测,并对制得的微采用扫描电子显微镜、动态光散射仪、流式细胞仪等进行表征,结果发现微的直径在1微米左右,尺寸较均一。

This experiment object was 1-cell embryo of KUNMING mouse , ROS was added in different developmental phases through basal medium with CZB medium, cultured to morulae, early blastula, blastula and the hatching of blastula, observed the change of cell number and cleavage index of embryo.

本试验以昆明小鼠1-细胞期胚胎为实验对象,以CZB培养液为基础液在不同发育阶段添加外源性ROS,培养至桑椹胚、早期胚、胚、扩张胚阶段,分别观察胚胎的胚细胞数、分裂指数的变化。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分,第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究,确立了一种较好的培养方法:与颗粒细胞共培养,找到了一种适合猪卵母细胞体外成熟的培养基:mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸,成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%,国外报道的最高成熟率为(85.7±4.1)%;第二部分对猪卵母细胞孤雌激活的化学方法进行了研究,确立了化学激活猪卵母细胞的两种最佳方法:1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min,再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中,卵裂率和桑胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min,再与8mM的DTT共孵育30min,卵裂率和桑/胚形成率为(81.0±2.8)%和(39.6±2.7)%;第三部分对孤雌激活胚胎的培养条件进行了研究,确立了一种最佳的胚胎培养条件:在SOF简单培养基中添加颗粒细胞进行前3天的培养,然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养,其桑椹胚和胚的发育率为(59.5±3.2)%;第四部分研究了IGF-I

There was no typical Golgi complex in oogonia, but there were some Golgi vesicles. At the early stage of vitellogenesis, Golgi complex was abundant whose saccules increased to more than ten layers. At the middle stage of vitellogenesis, Golgi complex grew more in numbers and got together, so its secreting quickened. Moreover, it formed a number of Golgi vesicles, which were filled with granule substances that changed into yolk granule finally.

卵原细胞内无典型的高尔基复合体,但具高尔基泡;卵黄发生早期,高尔基复合体发达,膜数达10多层;卵黄发生中期,高尔基复合体分泌活动加快,产生大量的大小泡,泡内充满颗粒状物质,最后演变成卵黄颗粒。

Four substances including 2-methyl ethyl acetoacetate and barley straw extract distilled from bulrush as well as cetyl trimethyl ammonium bromide and isothiazo lintone were used for the comparison experiment in inhibiting the growth of Microcystis aeruginosa in various phases. Result showed that inputting chemicals in the lag phase has better effect than in the log phase, and adding in lag phase can effectively inhibit the algae growth. Although feeding allelopathic substance at log phase can lead to certain restraining effect, it cannot result in the effective inhibition of algae growth, which can be well achieved by feeding CTAB and isothiazo lintone below 10mgl/L during log phase.

研究用从芦苇中提取的2-甲基乙酰乙酸乙酯和大麦秸浸出液两种化感物质以及十六烷基溴化钱和异噻唑啉酮等四种药剂对不同生长期的铜绿微藻进行了对比抑制试验,结果显示在铜绿微藻生长的迟缓期投加试验药剂效果比在对数期投加效果都好,在迟缓期投加四种药剂,都有很好的抑藻效果;在铜绿微藻生长的对数期投加化感物质,虽然有一定的抑藻率,但效果较差,而在藻对数期投加10mg/L以下的CTAB和异噻唑啉酮能达到很好的水华抑制效果。

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