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The preparation of phenyl chloroformate and chloroformic acidl,3-thiazole-5-ylmethyl ester with biscarbonate was studied.

然后研究了双碳酸酯在合成氯甲酸苯酯和氯甲酸-1,3-噻唑基-5-甲酯的应用。

Thiazolidiane-4-carboxylic acid was prepared and used as the aid catalyst that has strengthened the catalytic effect of chloroplatinic acid, greatly improved the yield of the hydrosilylation of methyldichlorosilane with allychloride, and made the yield get to 64.9%.

通过比较,选用自制的含硫化合物4-羧基-1,3-噻唑烷作助催化剂,大大增强了氯铂酸的催化活性,使甲基二氯硅烷与氯丙烯的加成反应以较高产率得到γ-氯丙基甲基二氯硅烷,产率可达64.9%。

The 6-nitro-2-benzothiazolyldiazoaminoazobenzene as a chromogenic reagent was synthesized and its color reaction with nickel has been investigated.

合成了显色剂6 硝基 2 苯并噻唑重氮氨基偶氮苯,并在TritonX 100表面活性剂存在下,研究了其与镍的显色反应。

HepG2 cells were exposured to oxymatrine, final concentrations of which were 0.1, 0.2, 0.3, 0.4, 0.5, 0.8, 1, 2, 5, 8, 10, 15 g.L-1 respectively, MTT method was used to determine the inhibiting effects of oxymatrine on HepG2 cells proliferation, followed by Haematoxylin and Eosin staining to observe the cell morphology.

以0.1,0.2,0.3,0.4,0.5,0.8,1,2,5,8,10,15 g.L-1的氧化苦参碱处理HepG2细胞,四噻唑蓝法检测其对细胞增殖的影响;苏木精-伊红染色比较细胞形态差异;免疫组化染色进一步检测HepG2细胞肿瘤相关蛋白表达量的变化。

The results showed polymeric diaryliodonium salts were effective aryl ion transfer reagents. Thirdly, a new type polymer supported hypervalent iodine reagents poly { [4-Hydroxy iodo] styrene} has been developed. It was an effective oxidative reagent, and could be applied to the one pot synthesis of 4-arylthiazoles.

然后,我们以聚苯乙烯羟基对甲苯磺酰氧基碘苯为原料,成功制备了一类新型聚合物高碘试剂—聚苯乙烯负载的炔基碘盐,并研究了其作为炔基转移试剂的有效性:(1)该试剂可对硫、氮、硒等负离子进行亲电炔基化反应,合成炔基亚砜、0,0-二烃基二硫代磷酸炔基酯、1-苯乙炔基苯并噻唑以及炔基硒醚。

Methods BGC823 cells were co-cultured with the wild type H.pylori and the isogenous mutant, which were called the wild tue strain group and the mutant stain group,respectively. The cell phenotype was observed at 6,12,24 and 48h and the cellular proliferation was examined by methyl thiazolyl tetrazolium method.The induction of apoptosis was determined by flow cytometry.

将幽门螺杆菌野生株与cagA基因缺失突变株与BGC823细胞共培养,分别在6,12,24和48h观察细胞形态学变化,采用四甲基偶氮噻唑蓝比色法检测细胞增殖活性,流式细胞仪检测细胞凋亡情况。

Methods BGC823 cells were co-cultured with the wild type H.pylori and the isogenous mutant,which were called the wild tue strain group and the mutant stain group,respectively.The cell phenotype was observed at 6,12,24 and 48h and the cellular proliferation was examined by methyl thiazolyl tetrazolium method.The induction of apoptosis was determined by flow cytometry.

将幽门螺杆菌野生株和cagA基因缺失突变株和BGC823细胞共培养,分别在6,12,24和48h观察细胞形态学变化,采用四甲基偶氮噻唑蓝比色法检测细胞增殖活性,流式细胞仪检测细胞凋亡情况。

Firstly, polyacrylamide with 26 million molecular weight was compounded in lab through establishing optimum compounding initiator proportionality of 1:1. 3 and mass concentration of 170 mgL^(-1), temperature of 10 ℃, acrylamide mass percentage of 25%, electrical conductivity of 3.6 μSm^(-1) and pH value of 6.57. The salt-resistance, thermal endurance and shear-resistance of polyacrylamide could be enhanced b introducing sulfonic acid group or styrene sulfonate group or drawing the two groups above at the same time. Secondly, chelator HEDP, bactericide isoquinoline ketone and oxygen scavenger thiourea were optimized to improve the compatibility of polyacrylamide and formation water.

在由室内试验确定了复合引发剂最佳比值1:1.3和质量浓度170 mgL^(-1)、合成最佳温度10℃、丙烯酰胺单体质量分数25%、电导率3.6μSm^(-1)和pH值6.57后,合成出相对分子质量高于2.6×10^7的聚丙烯酰胺,同时通过丙烯酰胺单体复配引入磺酸基团、苯乙烯磺酸基团或同时引入此两种基团,提高了聚合物的抗盐、抗温、抗剪切性能;后针对聚丙烯酰胺与地层水配伍性差的问题,优选出HEDP螯合剂、异噻唑烷酮杀菌剂和除氧剂硫脲,对聚合物稳定效果明显。

Results At different time and concentration,genistein had different effects on osteoblastic cell proliferation.

用四甲基偶氮噻唑蓝比色法检测细胞增殖,碱性磷酸酶活性测定检测细胞的分化。

Methods K-ras gene point mutation and its style at codon 12 of Patu 8988 cell were detected by using PCR-SSP and sequence analysis.

方法顺序特异引物聚合酶链反应法和基因测序检测Patu8988细胞K-ras点突变形式,根据点突变形式设计并合成硫代反义寡核苷酸(K-ras mutation ASODN)作用Patu 8988,通过噻唑蓝比色法检测细胞生长情况;流式细胞术检测K-ras蛋白表达和细胞凋亡;逆转录-聚合酶链反应检测K-ras mRNA表达水平;以Patu8988细胞建立裸鼠胰腺癌模型观察K-ras mutation ASODN在体内的抗肿瘤效果。

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