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Objective To observe the fluctuation of uric acid in serum on mouse after replicating model by using hypoxanthine and uricase inhibitor. Make certain the most proper approach and solvent for using uricase inhibitor.

目的 本实验考察次黄嘌呤、尿酸酶抑制剂复制大鼠代谢性高尿酸血症模型，观察造模后受试动物血清尿酸的变化，以确定尿酸酶抑制剂造模的最佳造模途径和溶剂。

Result:The linear of inosine is 0.040 1～0.721 8 μg,r=0.999 9,the limit of detection is 8.2 ng;The linear of hypoxanthine is 0.050 2～0.903 6 μg,r=0.999 9,the limit of detection is 7.4 ng.Conclusion:The peak ...

结果：肌苷的线性范围为0.040 1~0.721 8μg，r=0.999 9，最低检测限为8.2 ng；次黄嘌呤的线性范围为0.050 2~0.903 6μg，r=0.999 9，最低检测限为7.4ng；结论：各有关物质峰与肌苷峰能达到有效分离，且操作方法简便、准确、稳定，专属性强，可用于肌苷口服溶液有关物质的检查。

Results Hypoxanthine was administered via stomach, while unease inhibitor was injected subcutaneously by lanolin mixed with olefin, which made the uric acid in serum higher than control group about 12 hours.

结果 采用灌胃给予次黄嘌呤，皮下注射以羊毛脂石蜡油为溶剂配制的尿酸酶抑制剂，可使受试动物血尿酸值明显高于空白对照组(P.01)，且可维持12h。

Objective: Using HPLC To determine hypoxanthine in co-hirudo injection for establishing its HPLC fingerprint, and evaluating its internal quality. Method: The chromatographic separation was performed on a Kromasil C18 column (4.6 mm×250 mm,5 μm).

中文摘要：目的：用高效液相色谱法测定复方水蛭注射液中次黄嘌呤的含量，并建立注射液指纹图谱，全面评价其内在质量。

Two modified beacons containing of uracil and hypoxanthine have been used to validate the assay on purified proteins with success. However, while applying the assay to cell extracts, the results were interfered by non-specific nuclease activities.

我们分别设计其中含有尿嘧啶以及inosine的分子信号，以测定尿嘧啶-核酸醣解酶（uracil-DNA glycosylase）以及次黄嘌呤-核酸醣解酶（hypoxanthine-DNA glycosylase）的活性。

It can be used as a reference method for the determination of intracellular concentration of 6-thiopurine in clinical medicine.

可作为临床细胞内6-巯基嘌呤测定的一种参照方法。

The isolated working heart model was set up, high-performance liquid chromatography were used to measure myocardial adenosine triphosphate,total adenine nucleotides, while left ventricular end-diastolic pressure,left ventricular developed pressure, and rate of change of left ventricular pressure were determined before and after preservation.

对照组用St Thomas停搏液停搏并保存大鼠心脏6小时；实验组在St Thomas液中加入前列腺素E1(5 μg/L)停搏并冷保存6小时，利用离体鼠心非循环式Langendorff灌流功能测定模型，测定左心室舒张期末压、左心室发展压和左心室压力变化率，并检测心肌细胞线粒体三磷酸腺苷、腺嘌呤核苷酸总量。

The results showed that the leaves differentiation mode of Kalanchoe tomentosa is special. The green globoids were induced only on MS medium with additional 1.5 mg/L 6-BA and 0.5 mg/L IBA.

试验结果表明：褐斑伽蓝叶片分化方式特殊，初代培养时外植体叶片分化困难，仅在附加6-BA（6－苄基腺嘌呤）1.5mg/L、IBA0.5mg/L的MS培养基上分化出绿色球状体。

In 1984, Kirkman and Gaetani (1984) reported tightly bound NADPH in BLC, which then was also detected in the X-ray structure of BLC (Fita and Rossmann, 1985a). In the course of various sequencing projects a broad spectrum of catalase sequences from organisms of all kingdoms appeared in the public databases in the last 17 years e.g.

Vainshtein等获得了在3.5A 低等真核细胞中的过氧化氢酶的第一个电子云图，同时Eventoff 也得到了在2.5A处，用X-ray详细分析附面层控制图，1984年Kirkman 和报道了 Gaetani BLC中的紧密结合的还原型烟酰胺腺嘌呤二核苷酸磷酸

The leaves, leafstalk, and stems from grapes were used as explants to induce calli and subculture in medium (100 mL B5 medium containing 3 g sucrose and 0.6 g agar) with different plant hormone combinations (6-BA, KT, IBA, 2, 4-D), and then the resveratol content from those calli was determinated by using HPLC.

以葡萄的叶、叶柄和茎分别作为外植体，在不同激素组合（6-卞氨基嘌呤(6-BA)、激动素、吲哚丁酸、2，4-二氯苯氧乙酸(2，4-D)）的培养基（以B5培养基为基本培养基，含30g/L蔗糖和6g/L琼脂）上，进行愈伤组织的诱导和继代培养，并利用高效液相色谱法对不同来源愈伤组织中的白藜芦醇含量进行测定。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐