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The chemical speciation of elemental sulfur impacted by various SOB was very poorly investigated, further research may provide theoretical support to the elucidation of sulfur metabolism of acidophilic SOB.

有关嗜酸硫氧化细菌作用下硫的形态研究十分缺乏,进一步开展嗜酸硫氧化细菌作用下元素硫化学形态的研究可以为这类细菌的硫氧化机制及对硫化矿的浸出机理的阐明提供理论依据。

The effects of the surfactants on bioleaching of chalcopyrite were evaluated by measuring the leaching rate of copper and the density of bacteria cells in solution.

通过对浸矿溶液中铜的浸出率以及细菌浓度的测定来评价表面活性剂对氧化亚铁嗜酸硫杆菌浸出效果的影响。

You see, yogurt contains "Lactobacillus acidophilus" which is a bacteria naturally found in a healthy vagina.

你看,酸奶中包含"嗜酸乳杆菌"这是一个自然的细菌中找到一个健康的阴道。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象,提取细菌基因组DNA,用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切,回收大小为1~5Kb的DNA片段,将其连接入原核表达载体pET-28a-c,最后转化宿主菌,结果成功地构建了基因表达文库,为后续的基因筛选工作奠定基础;另外,本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计,以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板,进行PCR扩增反应,结果表明成功地获得两个新基因:23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸),并进一步做了不同物种之间的分子系统发育分析,构建了基于23S rRNA基因的邻接法系统发育树,阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

P 0.05. Total ruminal bacteria, proteolytic and amylolytic bacteria, and protozoa in rumen enumerated by culture method all increased (P 0.05) in the Trial period. Real-time PCR analysis was used to compare Pre-trial and Trial populations of cellulolytic species, including Butyrivibrio fibrisolvens, Ruminococcus flavefaciens and Ruminococcus albus, which were increased by 7.0, 4.5 and 9.6%(P 0.05), respectively. Two starch-fermenting bacteria species, Streptococcus bovis and Ruminobacter amylophilus, were increased by 25.4% and 14.6%(P 0.05), respectively.

3.02,P 0.05。传统培养法结果表明,与对照期相比,BSN2处理期奶牛瘤胃中瘤胃细菌、蛋白分解菌、淀粉分解菌和原虫数量显著增加;实时定量PCR结果表明,试验期奶牛瘤胃纤维分解菌,包括溶纤维丁酸弧菌、黄色瘤胃球菌和白色瘤胃球菌数量分别提高6.97%、4.51%和9.6%(P 0.05),两种淀粉分解菌,牛链球菌和嗜淀粉瘤胃杆菌分别提高25.4%和14.6%(P 0.05),乳酸利用菌埃氏巨球型菌也提高8.85%(P 0.05)。

Results The phenotypical features of galactococcus MG 1363, IL 1403 and Lactobacillus ATCC 4356 accorded with that in the Bergey's manual of determinative bacteriology.

结果 乳酸乳球菌乳脂亚种MG 1363、IL 1403及嗜酸乳杆菌ATCC 4356其表型特征分别与伯杰氏手册中相应细菌特征一致。

Refreshing agent contained 0.25% chitosan, 1% sodium lactate, 1% lactic acid and 250 mgkg^(-1) Nisin and MAP were used to determine microbial flora counts, psychrophilic bacteria counts, TBARS, carbonyl content and a* value of chilled meat to evaluate the effect of refreshing agent and MAP on chilled meat.

将复合保鲜剂(0.25%壳聚糖+1%乳酸钠+1%乳酸+250mgkg^(-1) Nisin)与气调包装共同使用,测定冷却肉的细菌总数、嗜冷菌数、TBARS值(硫代巴比妥酸值,Thiobarbituric Acid-reacitive Substances)、羰基含量以及红度值,来综合评价保鲜剂与气调包装复合后对冷却肉的影响。

You see, yogurt contains "Lactobacillus acidophilus" which is a bacteria naturally found in a healthy vagina.

你看,酸奶中包含&嗜酸乳杆菌&这是一个自然的细菌中找到一个健康的阴道。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?