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The acidophile s composition and community structure in the sediment was studied with 16S rDNA clone library and denaturing gradient gel electrophoresis.

采集了酸性矿山废水区域酸性沉积物样品,运用16S rDNA克隆文库技术和变性梯度凝胶电泳技术,研究了沉积物中的嗜酸菌类群以及它们的群落结构。

To establish effective methods for selective isolation of acidophilic filamentous actinomycetes from acidic soils, and to investigate their genus and species diversity.

针对酸性土壤中的嗜酸丝状放线菌,建立有效的选择性分离方法,并了解其多样性。

In this study, 252 acidophilic actinomycetes were isolated from 10 acidic soil samples collected in Jiangxi Province, Yunnan Province and Beijing with dispersion and differential centrifugation approach.

实验中,从江西、云南、北京采集的10份酸性土样,采用分散和差速离心法和常规分离方法分离出嗜酸放线菌252株。

In this thesis , the effects of acid materials on calcareous soil were studied by using laboratory soil trial and pot experiments trail. The text studied effects of acid materials on pH value in calcareous soil ; studied effects of phosphoric acid (H2SO4 as control) on pH value and micronutrient availability ; studied relation between P and available Fe, Zn in plant; studied effects of acid material on growth of acidophilic plant.

本文采用室内模拟试验和盆栽试验相结合的方法,研究了不同酸性物质对石灰性土壤的酸化效果;磷酸和硫酸对石灰性土壤pH值及微量元素有效性的影响;植物体内磷、有效铁、锌的关系;不同酸性基质对嗜酸植物生长的影响。

The selective isolation approach established here is robust for isolating various acidophilic filamentous actinomycetes from soil. Acidic soils in Jiangxi Province harbor abundant and diverse acidophilic actinomycetes.

所建立的选择性分离方法可用于土壤嗜酸丝状放线菌的高效分离;江西酸性土壤含有丰富多样的嗜酸丝状放线菌种属。

By means of the electron microscopic enzymo cytochemistry, it was observed that the activity of acid phosphatase was localized on the autophagic vacuole, the enzyme reaction granules gather to form the vacuolar bodies of various shapes in the cytoplasmic region of the osmiophilic and paraglycogen granules in resting Stylonychia mytilus .

该文应用电镜酶细胞化学方法显示,贻贝棘尾虫休眠包囊中,在胞质嗜锇粒和拟糖原粒区,酸性磷酸酶反应颗粒聚集于具有消化泡作用的泡状体上,葡萄糖 6 磷酸酶反应也定位在自噬泡膜位置。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色:翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成;基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质,在翼状胬肉体部深层基质中存在粗糙的纤维组织;正常球结膜组织细胞排列整齐;基质为疏松结缔组织,胶原纤维平行排列,其间可见成纤维细胞,散在少量中性粒细胞、毛细血管;Masson染色:翼状胬肉浅层基质中存在致密的胶原纤维染色,深层基质中的胶原纤维存在变性样改变;VG染色:翼状胬肉组织深层基质中存在大量变性的胶原纤维,其间夹杂黑色的弹性纤维;免疫组化染色法:MMP-3在翼状胬肉上皮细胞中呈强表达,成纤维细胞中呈中等强度表达,血管内皮细胞中未见表达;LN在血管壁中呈强表达,在上皮细胞基底膜中呈中等强度表达,在整个基质中未见明显表达;col Ⅲ在整个翼状胬肉基质中呈强表达。

ObjectiveTo observe the influence on IgE、 EOS、 IL-5、 IL-12 and clinical effect of ChuanFuLingTieJi by applying on the points (Dazhui 、 Dingchuan、 Feishu、 Pishu 、 Shenshu) of children patients with asthma,and compare with the ChuanFuLing-paste, researching the mecharism of CFLTJ and providing theoretical basis for feasibility of CFL form improvement and prevention and treatment of asthma.

通过观察中药喘敷灵贴剂三伏穴位(大椎、双侧定喘、双侧肺俞、双侧脾俞、双侧肾俞)敷贴对缓解期哮喘患儿临床症状、非特异性免疫球蛋白E、嗜酸性粒细胞、白细胞介素5(IL-5)、白细胞介素12(IL-12)的影响,并与疗效确切的喘敷灵传统剂型比较,探讨中药喘敷灵贴剂穴位敷贴防治缓解期小儿哮喘的临床疗效及其可能的作用机理,从而为中药喘敷灵剂型改良的可行性及其临床推广应用提供依据。

The probe of chip includes the entire genomic DNA of at least ten thiobacillus ferrooxidans, at least two thiobacillus acidophilus sulfur oxides, at least one eosinophils thiobacillus, at least one acidithiobacillus albertensis bacteria, at least six thiobacillus oxidation of ferrous - Lo, at least eight heterotrophic bacteria acidophilus, at least one metal leaf sulfur bacteria, at least one Acidianus tengchongensis, at least a Metallosphaera sedula and at least two of thermophilic bacillus curing.

该芯片的探针包括至少10株氧化亚铁硫杆菌、至少2株嗜酸氧化硫硫杆菌、至少1株喜温嗜酸硫杆菌、至少1株Acidithiobacillus albertensis菌、至少6株氧化亚铁微螺菌、至少8株异养嗜酸菌、至少1株金属硫叶菌、至少1株嗜酸两面菌、至少1株勤奋金属球菌和至少2株嗜热硫化杆菌的全基因组DNA。这种芯片,能够检测酸性环境中丰度较低的微生物,具有高通量、同时、快速、准确、灵敏地分析酸性环境中微生物群落组成的优点。

On the basis of phylogenetic analyses, three isolates, strain 117,43401 and 12202 were subject to a polyphasic taxonomic study.

实验结果显示,酸性土壤中的嗜酸稀有放线菌具有较丰富的种属多样性,其中诺卡氏菌属是一优势菌群。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

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