嗜热的

In conclusion, the lag phase of Streptococcus thermophilus was shorten in the eutrophy culture medium, and the time achieving the culminated point of logarithmic phase was shorted also, at the same time, the stationary phase lengthened. After secondary culture, Streptococcus thermophilus reached directly the log phase hardly through the lag phase, and then quickly reached the decline phase. There were more relations of the metabolic regalities of the three kinds of nutritive substances with the super-concentrated incubation process of Streptococcus thermophilus: the more the number of the bacteria, the quicker the metaboling, while the fewer, the slower the metabolism. Moreover, there apparently existed morphologic changes in this course, and there maybe existed the apoptosis, which correlated with bacteria propagation and acid producing. With the culture time postponed, the nutritive substances in the culture medium lacked also, and the morphologic change and apoptosis appeared more obviously.

结果表明，嗜热链球菌在营养丰富的培养基中的生长延滞期缩短，到达对数生长顶点的时间变短，稳定期的时间延长，当经过二次培养后，嗜热链球菌几乎不经过延滞期直接到达对数期，平稳期持续的时间较短，很快到达衰亡期；嗜热链球菌在超浓缩培养过程中三大营养物质的代谢规律也同它的培养过程有很大的关系，菌数多，其代谢旺盛，菌数降低其代谢速度减慢；嗜热链球菌在超浓缩培养过程中存在着明显的菌体形态变化，并可能存在着细胞凋亡，与菌体增殖和产酸规律密切相关，随着培养时间延长，培养基中营养物质变得贫乏，菌体形态变化和细胞凋亡的情况愈加明显。

L. acidophilus1. The proper process conditions were studied of the blended fermented milk of function L.

对功能性嗜酸乳杆菌和嗜热链球菌混合菌种发酵乳的最佳生产工艺条件进行研究。

EPS composition fermented with strain 7 and Streptococcus thermophilus together was stable and consists of glucose, galactose and rhamnose, but EPS with normal starters of Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus does not consist of rhamnose.

产粘7号菌在诱变前后与不产粘嗜热链球菌共同发酵产生的EPS单糖组成不变，都是由葡萄糖、半乳糖和鼠李糖组成；而不产粘嗜热链球菌与不产粘保加利亚乳杆菌产生的EPS不含鼠李糖，只有葡萄糖和半乳糖。

As thermophilic mycelial fungus, Sporotrichum thermophile can decompose cellulose.

嗜热侧孢霉是一种嗜热丝状真菌，具有分解纤维素的特性。

Thermoanaerobacter tengcongensis is a rod-shaped, Gram-negative, anaerobic eubacterium that was isolated from a fresh water hot spring in Tengchong, China. Using a"whole-genome-shotgun"method, we sequenced its 2, 689, 445-bp genome from an isolate, MB4〓(Genbank Accession Number AE008691). The genome encodes 2, 588 predicted coding sequences . Among them, 1, 764 (68. 2％) are classified according to homology to other documented proteins, and the rest, 824 CDS (31. 8％), are functionally unknown. One of the interesting features of the T. tengcongensis genome is that 86. 7％ of its genes are encoded on the leading strand of DNA replication.

中文题名腾冲嗜热厌氧菌全基因组测序及分析副题名外文题名 A complete sequence of the T.tengcongensis genome 论文作者包其郁导师杨焕明研究员学科专业遗传学研究领域\研究方向学位级别博士学位授予单位中国科学院遗传与发育生物学研究所学位授予日期2002 论文页码总数88页关键词腾冲嗜热厌氧菌基因组嗜热菌馆藏号BSLW /2003 /Q939.1 /33 腾冲嗜热厌氧菌（MB4＇）是分离于我国腾冲地区温泉内的革兰氏阴性厌氧真细菌。

At the present time, It is a major method of research on the thermostability of thermozymes to compare the homology of amino acid sequence between thermozymes and mesophilic enzymes.

螺旋的稳定性使嗜热酶在二级结构上更稳定。在高级结构中，&额外&的离子键、疏水作用、氢键、寡聚体的形成等对嗜热酶的耐热性起主要作用。

Full enzymatic synthesis of N-protected Leu-enkephalin was successfully achieved via 3+2synthetic route by α-chymotrypsin and thermolysin, and the Z-protected Leu-enkephalin phenylhydrazine was obtained in tert-amyl alcohol by thermolysin for the first time.

以3+2的合成路线使用α-胰凝乳蛋白酶和嗜热杆菌蛋白酶完成了N-保护的亮-脑啡肽的酶法全合成，并在第三戊醇中首次以嗜热杆菌蛋白酶为催化剂得到了Z-保护的亮-脑啡肽酰苯肼衍生物。

By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制，酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力，酶蛋白亚基间的相互作用，酶蛋白的折叠以及内运动状态与酶活力的关系，酶蛋白发挥功能时的结构特性；运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性，热稳定性机制；确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位；确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构；确定了一个新型的染色质蛋白Cren7的溶液三维结构；正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

To obtain thermostable xylose isomerase, the gene xylA from an extremely thermophilic bacterium,Thermus thermophilus HB8 was cloned and its product was overexpressed in Escherichia coli BL21(DE3) with expression vector pET22b.

为获得具有高热稳定性的木糖异构酶，运用基因工程技术，从嗜热栖热菌Thermus thermophilus HB8中克隆到嗜热木糖异构酶基因xylA。

The probe of chip includes the entire genomic DNA of at least ten thiobacillus ferrooxidans, at least two thiobacillus acidophilus sulfur oxides, at least one eosinophils thiobacillus, at least one acidithiobacillus albertensis bacteria, at least six thiobacillus oxidation of ferrous - Lo, at least eight heterotrophic bacteria acidophilus, at least one metal leaf sulfur bacteria, at least one Acidianus tengchongensis, at least a Metallosphaera sedula and at least two of thermophilic bacillus curing.

该芯片的探针包括至少10株氧化亚铁硫杆菌、至少2株嗜酸氧化硫硫杆菌、至少1株喜温嗜酸硫杆菌、至少1株Acidithiobacillus albertensis菌、至少6株氧化亚铁微螺菌、至少8株异养嗜酸菌、至少1株金属硫叶菌、至少1株嗜酸两面菌、至少1株勤奋金属球菌和至少2株嗜热硫化杆菌的全基因组DNA。这种芯片，能够检测酸性环境中丰度较低的微生物，具有高通量、同时、快速、准确、灵敏地分析酸性环境中微生物群落组成的优点。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。