嗅神经

We choose abortus of three to six months old as research object. In the research, we collected 35 specimen that all came from induction of labor with water bag. We successfully culture OECs from human embryo olfactory bulb by using primary culture.

一、人胚胎嗅神经鞘细胞的体外培养本研究共收集标本35例，均为3—6个月的水囊引产流产儿，采用原一代培养的方法，自人胚胎的嗅球培养出OECS。

Materials and Methods:MRI findings of 183 cases with cranial nerve tumors including 135 acoustic nerve tumors,35 tregemimal nerve tumors, 3 olfactory nerve tumors, 3 optic nerve tumors, 3 hypoglossal nerve tumors, 2 vagus nerve tumors, 1 facial nerve tumor and 1 glossopharyngeal nerve tumor verified by operation and pathology were analyzed. Results: Of the cranial nerve tumors,177 were benign tumors,6 were malignant tumors.

材料和方法：收集了183例资料完整，均经手术和病理证实的脑神经肿瘤病例，其中嗅神经肿瘤3例，视神经肿瘤3例，三叉神经肿瘤35例，面神经肿瘤1例，听神经肿瘤135例，迷走神经肿瘤2例，舌咽神经肿瘤1例，舌下神经肿瘤3例。

Objective: To study the clinicopathological characteristics of olfactory neuroblastoma in human nasal cavity.

目的：探讨鼻腔嗅神经母细胞瘤的临床病理学特征，提高本病的诊治水平。

No electrically evoked potentials were detected on the respiratory mucosa, nor did on the olfactory mucosa after olfactory neurectomy.

电极置于呼吸区粘膜及切断嗅神经后的嗅区粘膜不能引出诱发电位。

Objective: To review the diagnosis, treatment and prognosis of olfactory neuroblastoma.

目的：探讨嗅神经母细胞瘤的诊断和治疗及预后。

Purpose: To analyze treatment outcome and prognostic factors of patients with olfactory neuroblastoma.

目的：研究嗅神经母细胞瘤患者之治疗结果与预后因子。

METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.

在差速贴壁+Thy1.1抗体及补体纯化法的基础上，剪开大鼠颅骨，显露位于颅腔前方的嗅球，取出2只嗅球，在显微镜下去除嗅球表面的软脑膜和毛细血管及外周组织，保留富含嗅鞘细胞的嗅神经层和嗅球颗粒层，剪成1 mm3小块分离获取单细胞悬浮液，调整细胞密度至1×107 L-1，接种在用poly-l-lysine包被的培养瓶或培养板中，于37 ℃、体积分数为5%的CO2培养箱中培养，第3天用无血清DMEM/F12培养基换液培养。

Methods: The olfactory nerve layer and glomerular layer was isolated from the olfactory bulb of three-day old rats, Enzyme digestion method was used to isolate cells and the cells were purified by differential adhesion method. Freshly isolated OECs were plated onto poly-L-lysine-coated tissue culture and then incubated for 2 days. The purity of OECs was identified by immunocytochemistry of NGFRp75 and S100 and Hoechst33342 counterstain method.

从新生SD大鼠(3d)嗅球中迅速分离嗅神经层和嗅颗粒层，采用酶消化法分离细胞，差速贴壁法纯化细胞，接种于多聚赖氨酸包被的培养板内培养2d，采用NGFR p75和S100蛋白双标免疫组化、以Hoechst33342复染鉴定OECs的纯度。

Here we show that mouse horizontal basal cells function as adult olfactory neuroepithelium neural stem cells and examine their distinct dynamics in olfactory neuroepithelium maintenance and regeneration.

本文中我们发现小鼠水平基底细胞具备成体嗅神经上皮神经干细胞的作用，并研究他们在嗅神经上皮保持和更新的独特动力学特征。

Fate-mapping analysis after olfactory neuroepithelium lesioning shows that HBCs are competent to regenerate both neuronal and non-neuronal olfactory neuroepithelium lineages.

嗅神经上皮损害后细胞命运映射分析显示HBCs足以重建嗅神经上皮的神经元谱系和非神经元谱系。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。