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We choose abortus of three to six months old as research object. In the research, we collected 35 specimen that all came from induction of labor with water bag. We successfully culture OECs from human embryo olfactory bulb by using primary culture.

一、人胚胎嗅神经鞘细胞的体外培养本研究共收集标本35例,均为3—6个月的水囊引产流产儿,采用原一代培养的方法,自人胚胎的嗅球培养出OECS。

Methods: The olfactory nerve layer and glomerular layer was isolated from the olfactory bulb of three-day old rats, Enzyme digestion method was used to isolate cells and the cells were purified by differential adhesion method. Freshly isolated OECs were plated onto poly-L-lysine-coated tissue culture and then incubated for 2 days. The purity of OECs was identified by immunocytochemistry of NGFRp75 and S100 and Hoechst33342 counterstain method.

从新生SD大鼠(3d)嗅球中迅速分离嗅神经层和嗅颗粒层,采用酶消化法分离细胞,差速贴壁法纯化细胞,接种于多聚赖氨酸包被的培养板内培养2d,采用NGFR p75和S100蛋白双标免疫组化、以Hoechst33342复染鉴定OECs的纯度。

No abnormal features were found in cortex except for a malacia lesion in inferior frontal lobe in 1 case.

测量嗅球体积、嗅沟深度及垂体、垂体柄径线,并进行比较。

The imaging findings of post-trauma dysosmia commonly manifest as malacia and chronic hemorrhageic foci in frontal lobe, olfactory bulbs and tracts as well as temporal lobes.

外伤后嗅觉功能障碍的最常见影像学表现是额叶底部、嗅球嗅束和颞叶的软化灶、陈旧出血改变。

Glomeruli layer and granules layer of accessory olfactory bulb exist significant difference in the Cricetulus barabensis and Microtus mandarinus.

黑线仓鼠和棕色田鼠副嗅球中嗅小球层和颗粒层也存在明显的差异。

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜,剪碎后胰酶消化,制成单细胞悬液,按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中,18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中(第1次差速贴壁),24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中(第2次差速贴壁),接种培养48 h后半量换液以去除杂细胞。

Olfactory ensheathing cells were isolated from newborn rat olfactory bulb and cultured in vitro.

实验从新生大鼠嗅球中分离出嗅成鞘细胞,进行体外培养。

OBJECTIVE: To investigate the association between olfactory disturbance and ultrastructural mitochondrial changes in olfactory bulb neurons of aged rats in comparison with young rats.

目的:对衰老和年轻大鼠嗅球内神经元的线粒体超微结构进行对比观察,分析衰老大鼠嗅觉障碍与嗅球内神经细胞线粒体改变的关系。

Here we demonstrate that insulin-like growth factor signaling is required for sensory innervation of the lateral olfactory bulb.

嗅神经元会将轴突投射到嗅球的嗅觉蕾,从而形成嗅感觉地图。

Objective:To study the expression characteristics of NeuN(neuron-specific nuclear protein/neuromal nuclei) in the development of mice olfactory bulb and epithelium.

目的:探讨神经元核心抗原在嗅球和嗅上皮发育过程中的表达特性和意义。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?