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哺乳动物

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These fossils represent different major groups and show high diversity in body size, locomotory adaptation, and dietary.

这些化石代表了不同的哺乳动物大类,同时显示了在个体大小、运动姿态以及食性方面的较高的分异。

In the public ranking of marine mammals, dolphins are adored, whales revered, and seal pups make people swoon. But walruses remain perversely, lumpishly obscure.

在海洋哺乳动物人气排行榜中,海豚受喜爱,鲸受尊敬,小海升O人著迷,海象则始终别扭、笨重,叫人摸不透。

She was a mother because she was mammiferous.

她作了母亲,因为她是哺乳动物

The mammiferous testis perform important functions of producing sperm and secreting male hormone.

雄性哺乳动物的睾丸具有生精和分泌雄性激素等重要的功能。

Embryonic Stem cellscan be derived from the early stage em bryos of mammiferous animals.

哺乳动物早期发育阶段的胚胎中可以得到胚胎干细胞。

There are totally collect more than 100 kinds of mammiferous genitals in Hoosaweike's musuem which land in a fishing village of Iceland.

这家位于冰岛渔村胡撒威克的博物馆共收集了100多种哺乳动物的生殖器。

Objective: The genes were connected basing on obtain gene of MCP and DAF of liver and fetal marrow of Chinese adult and then design mammiferous vector of the hMCP-DAF fusion gene. Finally transfer hMCP-DAF fusion gene to the early embryo of jinhua''s pig.

目的 在获得中国成人肝脏、胎儿骨髓MCP、DAF基因克隆的基础上,进一步将两个基因串接,并构建hMCP-DAF融合基因的哺乳动物表达载体,筛选出具表达活性的hMCP-DAF并导入金华猪早期胚胎。

With the method of reverse transcription-polymerase chain reaction and nest PCR, P80 gene of CSFV is cloned from the cells infected by the strain Shimen of csfv, and connected with eukaryotic expression vector pEGFP-C1. Then recombined eukaryotic expression plasmid pEGFP-P80 of P80 gene is achieved successfully. It builds the basis for expressing and gaining protein p80 in mammiferous cells.

利用反转录PCR和套式PCR技术,从猪瘟病毒Shimen株细胞毒中克隆出P80 基因,并进一步把P80 基因连接在真核表达载体pEGFP-C1 上,成功地构建出P80 基因重组真核表达质粒(pEGFP-P80),为在哺乳动物细胞中表达并纯化出p80 蛋白奠定了基础。

Then pMD-P80 is digested by the enzymes Xho I and Apa I, separated and linked to the linear plasmid pEGPF-C1 after digested by Xho I and Apa I. So the recombined expression plasmid pGFP-P80 is achieved. Then it is sequenced, PCR and digested by the restriction enzymes Xho I and Apa I. The results give that pGFP-P80 is achieved successfully and the insert sites, direction and reading frame are all right. It builds the basis for expressing, purifying, gaining p80 protein from mammiferous cells.

将pMD-P80 分别经Xho I 和Apa I 双酶切和回收,然后与经过Xho I 和Apa I 酶解的真核表达载体pEGPF-C1 连接、转化,获得重组质粒,经PCR,Xho I 和Apa I 限制性酶切和序列测定,鉴定为真核表达质粒pEGFP-P80,并且目的基因的插入位置、方向和读码框完全正确,为在哺乳动物细胞中表达并分离纯化p80 蛋白奠定了基础。

The mice were used as experimental animals in this study,through the experiment in vitro and in vivo,systemic invested the dynamic changes in expression and localization of FGF7,FGF10 and its receptor in mammals development,lactation and involution;revealed the relationships between the expression of FGF7,FGF10 and its receptor and the function;clarified the function of FGF7 and FGF10 in mammary gland development,lactation and involution,and the effect of mammogenic hormones on expression of FGF7 and FGF-10 and its receptor in different periods in vitro.

本研究主要以小鼠为实验材料,通过体外和体内实验,系统研究FGF7、FGF10及KGFR在哺乳动物乳腺发育、泌乳及退化过程中表达定位的动态变化,揭示FGF7、FGF10及KGFR表达变化与乳腺发育及泌乳功能间的对应关系,阐明FGF7、FGF10在乳腺发育、泌乳及退化过程中的功能以及乳腺发育激素对不同时期乳腺FGF7、FGF10及KGFR表达的影响。

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