咖啡酸的

Caffeic acid is the most abundant of all the identified compounds in this work and this result is comparable with those reported in other papers.

咖啡酸是最丰富的所有已确定的化合物在这方面的工作，这是比较的结果与这些报告的其他文件。

Objective: To approach the effect of caffeic acid germanium to H22 hepatoma mouse and the mechanism of inducing cell apoptosis.

目的探讨咖啡酸锗对H22肝癌小鼠的体内抗肿瘤作用及其诱导细胞凋亡的机制。

Caffeic acid could resist the H2O2-induced cell apoptosis, with the possible molecular mechanism of regulating the NF-κB, P53 and BCL-2 expression.

不同浓度的咖啡酸对H2O2引起的HUVEC-12损伤有保护作用，并呈现一定的浓度依赖性和时效性，其机制可能与调节NF-κB、P53和Bcl-2蛋白的表达有关。

The effects of homogenization pressure and sodium caseinate dosage on the particle size distribution and stability of liquid coffee whitener were studied.

本文主要研究了酪朊酸钠用量、均质工艺条件对液态植脂咖啡伴侣的粒度分布及稳定性的影响。

The interaction between CaA and Cu and influence of ethylenediaminetetraacetic acid,solvent and pH on the interaction were also studied to help elucidate the detailed prooxidant mechanism using UV-visible spectroscopy, demonstrating that it is CaA phenolate anion,instead of the parent molecule,chelates with Cu as a bidentate ligand,hence facilitating intramolecular electron transfer between CaA and Cu.

可见分光光度计研究了CaA和Cu的相互作用以及EDTA、溶剂、pH对这种相互作用的影响，证实了CaA本身没有与Cu螯合，而是咖啡酸酚氧负离子作为双齿配体与Cu进行螯合，形成的络合物有利于分子内电子转移。

The results Showed that twenty isolates have the following biological aspects:① Capability of forming capsule,② Growth at 37℃ within 3days,③ Forming brown colonies on Niger seed agar, CACA agar,④ Urea hydrolysis test positive within 96 hours.⑤ No blue colonies were found on CGB agar.⑥ The genus could be identified by ID32 identification kid, but the species could not.⑦ The sequence of the LSUrDNA D1/D2 region analyzed confirmed to be C. neoformans var neoformans.⑧ Serotype A and AD were found in these isolates, Serotype A was predominant(83.33%),⑨ Some biodiversities of isolates existed.

结果 20株菌皆具有以下特征：①形成英膜，②37℃3天内生长试验皆为阳性，③咖啡酸玉米琼脂和油菊籽培养基皆可以产生褐色菌落，④尿素酶试验96h皆呈阳性，⑤CGB培养基未见蓝色菌落生长，⑥ID32鉴定板可以鉴定到属，种的鉴定结果各不相同，⑦大亚基核糖体DNA的D1/D2区域序列分析证实皆为新生隐球菌的新生变种，⑧血清型仅见A和AD二型，以A型为多数(83.33%)，⑨20株临床分离株随机扩增DNA多态性分析有一定的生态多样性。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

Results: Nine compounds were obtained from the ethyl acetate fraction of C. speciosa and were identified as methyl chlorogenate, 5-O-cafeoyl quinic acid butyl ester, quinic acid butyl ester, 5-hydroxymethyl-furan-2-carbaldehyde, triacontanoic acid,-epicatichin, 7,8-dihydroxy-coumanrin, protocatechuic acid ethyl ester, and protocatechuic acid.

结果：从皱皮木瓜的乙酸乙酯萃取部位分离鉴定了9种化合物，分别为绿原酸甲酯，5-O-咖啡酰基－奎宁酸丁酯，奎宁酸丁酯，5-羟甲基－2-糠醛，三十烷酸，-表儿茶素，7，8-二羟基香豆素，3，4-二羟基苯甲酸乙酯，原儿茶酸。

The dry matter weight accumulation of different organs in growth process and contents of scutellarin and coffeic acid ester in whole plant of E. breviscapus were determined.

观测了灯盏花植株的生长发育进程，全草及各部位的干物质积累规律和全草主要有效成分灯盏乙素和咖啡酸酯含量的积累规律。

These results corresponded to a higher lignin content measured by the Klason method and stem strength and a lower lodging index in H4564 than in C6001 at the heading and milky stages. Therefore, the TaCM mRNA levels, protein levels, and enzyme activity in developing wheat stems were associated with stem strength and lodging index in these two wheat cultivars.

马庆虎研究组分离了在小麦茎秆中高度表达的木质素合成关键酶基因-咖啡酸甲基转移酶基因，通过生化和转基因分析证明COMT 在控制松柏醇型木质素上发挥着重要作用，COMT基因的表达在抗倒伏小麦生长发育后期明显高于易倒伏品种，这种基因的高表达进一步促进了COMT酶蛋白和酶活力的提高，并增强了木质素的合成。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。