和硫

P28 紫色 Formation of a peptide bond 肽键形成–4侧链具有的反应–形成二硫键-其它反应 Tyr,His,Arg Review Isoelectric Point Titration滴定 of an amino acid 滴定 Gly幻灯片 18 His Lys Glu Reaction of amino acids Section 4 Protein isolation and purification 蛋白质分离及提纯 Working With Proteins Experimental techniques for protein analysis and characterization Purification steps A cell contains many types of proteins In the lab we want to isolate a single protein for experiments Purification steps We first grow cells or isolate tissues that contain the protein of interest We break open the cells to produce a crude extract Use centrifugation离心 to separate soluble from insoluble material We fractionate 分离 the protein mixture based on properties of such as size, charge affinity or solubility.

丹磺酰氯与氨基酸反应生成荧光性质强和稳定的磺胺衍生物，用于多肽链NH 用于多肽链 3末端氨基酸的标记烃基化反应(1) 2，4-二硝基氟苯2，4-dinitrofluorobenzene，二硝基氟苯(二硝基氟苯 DNFB也叫做试剂。DNFB在弱碱性溶液也叫做Sanger试剂试剂中与氨基酸发生取代反应，生成黄色化合物二硝基二硝基苯基氨基酸(dinitro phenyl amino acid， DNP氨基酸氨基酸)苯基氨基酸氨基酸(2)苯异硫氰酸酯(phenylisothiocyanate， PITC)在弱碱性条件下，与氨基酸反应在弱碱性条件下，生成苯乙内酰硫脲 PTH衍生物，(phenylthiohydantoin， PTH)衍生物，即PTH-氨基酸，此反应又称之Edman反应，该反应是蛋白质或多肽氨基酸序列测定常用的反应。

Drugs (methimazole, methylthiouracil, propylthiouracil and thiouracil); some sedatives (doxylamine succinate and phenobarbital); and some other drugs including the systemic antifungal antibiotic griseofulvin, the diuretic spironolactone, and the antibacterial sulfa drugs sulfamethazine and sulfamethoxazole.

抗甲状腺？药物（他巴唑，甲基硫氧嘧啶，丙基硫氧和硫氧嘧啶）；一些镇静剂（doxylamine琥珀和苯巴比妥），以及包括系统性真菌抗生素灰黄霉素，安体舒通的利尿一些其他药物，以及抗菌磺胺类药物和磺胺二甲嘧啶。

Based on the crystal structure of sulfonium salt and the reaction results, it was proposed that the β-hydroxy of the sulfonium salt play a key role in the highly stereoselective cyclopropantion reation.

根据硫盐晶体结构和硫盐在反应中所表现的性质，认为硫叶立德 b -位的羟基可能是反应取得高对映选择性的关键。

He main action of TTFD is due to the phosphorylation of the intracellular free thiamin to form thiamin pyrophosphate and thiamin triphosphate, thus catalyzing energy metabolism.

TFD 的主要功能来自于细胞内自由硫胺的磷酸化以形成硫胺磷酸盐和硫胺三磷酸盐，因此能促进能量新陈代谢。

Part two: Using chloroformic ester reacted with potassuim thiocyanate to get thiocyanic esters. Then reacted with biological substituted pyrimidinamine to synthesize forty two thiourea compounds, thirty eight of them are new compounds. Their structures have been charactered by IR, 1H NMR and elemental analysis.

第二部分：先以氯甲酸酯和硫氰酸钾为原料合成异硫氰酸酯，再与有生物活性的取代氨基嘧啶反应，合成了一系列新的取代嘧啶基硫脲类化合物，合成的42个化合物中有38个为首次合成的新化合物，结构经元素分析、IR及~1H NMR得到确证。

Bunte salt was first prepared by α-furfuryl chloride and sodium thiosulfate. Then under the reaction of Bunte salt with 2-methyl-3-furanthiol in the presence of sodium hydroxide, furfuryl 2-methyl-3-furyl disulfide was prepared.

首先以α-糠基氯和硫代硫酸钠反应制备Bunte盐，然后Bunte盐与2-甲基－3-呋喃硫醇在碱性条件下反应合成了产品糠基2-甲基－3-呋喃基二硫醚。

Theoretical studies on various geometrical structures of Hypophosphorous acid were carried out using DFT theoretical methods,at the B3LYP/6—311++G** level.Intramolecular proton transfer isomerization has also been studied using the intrinsic reaction coordinate.

采用量子化学密度泛函B3LYP方法，在6—311++G**基组水平上全优化得到了硫代次磷酸的硫酮式和硫醇式两种构型异构体以及相应的对映异构体，同时对分子内质子转移异构化反应微观机理进行了理论分析。

N-substituded thioacridones with five different structures were synthesized via a route involving Ull-man reaction, cyclization, N-alkylation and sulfurization. The structures of each thioacridone were identified with IR and MS. The results from UV-spectrophotometry show that the five N-alkythioacridones all have stronger absorption in the visible light region.

采用乌尔曼反应、酸催化环化、N-烷基化和硫代化四步反应路线合成了5种不同结构的N-取代硫代吖啶酮，利用IR和MS分析手段表征了其结构，UV分光光度计的测定结果表明5种硫代吖啶酮在可见光区都有较强的吸收。

The impure carbon dioxide which can be obtained from any available source of carbon dioxide will typically contain as impurities sulfur compounds such as carbonyl sulfide, hydrogen sulfide, dimethyl sulfide, sulfur dioxide and mercaptans, hydrocarbon impurities such as aldehydes, alcohols, aromatics, propane, ethylene, and other impurities such as water, and carbon monoxide.

可由任何二氧化碳源得到的低纯度二氧化碳通常含有以下杂质：硫化合物，例如硫化羰、硫化氢、二甲硫、二氧化硫和硫醇；烃杂质，例如醛、醇、芳族化合物、丙烷、乙烯；以及其它杂质，例如水和一氧化碳。

To attaining longlived charge separated states, the new compounds 7 and 8 of C60-S-2-thioxo-1, 3-dithioles derivatives were synthesized by Diels-Alder cycloaddition reaction. The structures generated by AM1 were used as the initial configurations for the density functional optimization at the B3LYP/3-21G level for all carbon and sulfur clusters, and some geometry conformation indexes were obtained. We found that C60 has been half-packed by the curved 2-thioxo-1, 3-dithioles derivatives.

为了获得具有长寿命电荷分离态的[60]富勒烯－富硫衍生物有机功能分子，我们设计了用单键硫桥键联[60]富勒烯和硫酮衍生物的分子7和8，用半经验AM1方法得出最低能量的几何结构，在此基础上用密度泛函 B3LYP/3-21G方法，对其进行计算，得出电子相关的优化参数，预测其稳定性。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。