命名为

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

They had begun idealistically, establishing a central town with resources for all the colonists to share, naming it Free Haven, and dividing farmland equally among the able-bodied workers.

于是他们抱着幻想开始了他们的生活，利用所有殖民者共有的资源建立了一个中央城镇并且把它命名为 Free Haven ，为每一个有劳动力的人平均分配了耕地。

Ferrari has been working on a proprietary part-time 4WD system designed for both its mid and front-engined models for the past several years, which the carmaker has christened 'Insertable 4x4'.

法拉利车队一直在努力专有部分时间四轮驱动系统设计为中期和前置引擎的模式在过去几年中，该汽车制造商命名为'插入四驱车。

Consisting of singer/guitarist Kele Okereke, guitarist Russell Lissack, bassist/singer Gordon Moakes, and drummer Matt Tong, the band was formerly known as Angel Range and Union before settling on Bloc Party.

乐队由歌手兼吉他手凯里·欧克瑞克、吉他手罗索·里匝克、贝司手兼歌手高登·牟克斯和鼓手麦特·唐组成，曾命名为&天使牧场乐队&和&联合乐队&，后来才定名为&联合党乐队&。

In order to understand the function of the Rab protein in ciliates, the coding region of Eorab1f was obtained from macronuclear DNA of Euplotes octocarinatus. Sequence analysis revealed that the gene contains 624 bp and two in-frame TGA codons in the coding region.

为进一步研究该家族成员的功能，本研究从八肋游仆虫大核基因组中克隆得到Rab蛋白家族中一个新Rab蛋白基因(命名为Eorab1f)的编码区。

A strain producing 2-oxoaldehyde reductase was isolated from LB culture medium containing methylglyoxal. By analyzing its physiological and biochemical characters, and 16S rDNA sequence, it was identified as S. maltophilia and named as Stenotrophomonas inaltophilia YL.

用含甲基乙二醛的LB培养基平板筛选到1株能代谢甲基乙二醛的细菌，通过对克隆到的16S rDNA基因序列比对及生理生化实验，将其鉴定为嗜麦芽寡养单胞菌，命名为Stenotrophomonas maltophilia YL。

Since the second half of this year, is Haoxiliantai, keep the good news: in Loudi organized by the children's song and dance competitions in the province, Zhangjiajie was one of the two gold silver and one bronze; held in Suzhou in the first national farmers will turn in literature and art, Sangzhi Lantern,"Yang Spring bird reminder "was third, 6 for the province's award-winning one; held in Changsha, Hunan harmony in the United States and the text reading contest, Zhangjiajie was the Gold Award, the Urban Culture Award by the organization; At the same time, also successfully held Zhangjiajie sing the "eco-tourism Cup" Xiang Xiang Song of the trials and Sangzhi China Minge Jie, Sangzhi County, was named the country's rural Chinese folk ......

今年下半年以来，可谓好戏连台，喜讯不断：在娄底举办的全省少儿歌舞大赛中，张家界获得一金两银一铜；在苏州举办的全国首届农民文艺会演中，桑植花灯《阳雀催春》获三等奖，此为全省6个获奖项目之一；在长沙举办的和谐湖南美文诵读大赛中，张家界获金奖，市文化局获组织工作奖；同时，还成功举办了唱响张家界&生态旅游杯&湘人湘歌选拔赛和中国桑植民歌节，桑植县被国家命名为中国民歌之乡……这是我市文化战线坚持科学发展观，解放思想，在对待非物质文化遗产上走以申报求保护、以传承求发展的新路子取得的令人瞩目的成果。

To elucidate the structural features and the antimicrobial activity of this fragment, we re-designed and synthesized two peptides corresponding to the main mimic structures of Mytilin-1 from Mytilus coruscus, we named these two peptides Mytilin Derived Peptide-1 and Mytilin Derived Peptide-2, respectively.

为验证该区域是否具有抗菌活性，通过对厚壳贻贝Mytilus coruscus抗菌肽Mytilin进行空间结构模拟，选取其中β-发夹部分肽段，采用了固相化学合成的方法合成了两条10肽，分别命名为Mytilin Derived Peptide-1 (MDP-1)和 Mytilin Derived Peptide-2 (MDP-2)。

A phenylalanine ammonia-lyase gene was clone from Nerium oleander for the first time and it was also the first cloned gene involved in anthocyanin metabolic pathway in N.oleander.

首次从夹竹桃中克隆得到苯丙氨酸解氨酶基因cDNA片段，并命名为NoPAL1，GenBank登录号为AY747677，这也是首次从夹竹桃花青苷代谢途径中克隆得到的基因。

Based on his analysis of the stone tools from late Palaeolithic to early Neolithic period in Lingnan area(to the south of the Wuling Ranges),the author tries to separate acharacteristic stone assemblage and names it as Huangyandong culture.

本文通过对岭南地区从旧石器时代晚期到新石器时代早期一批重要遗址的石器进行文化分类排序的尝试，归纳出一套具有典型特征结构的石制品组合，并将以这套石制品组合为代表的遗存命名为&黄岩洞文化&。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐