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At no time, and its various parts can be contraction folded, gather together, into a oval thing, its surface smooth and rich luster, that is the reason why it will be named "Dining Pod"; while during mealing time, just to pulled out chair, will be hoisted up the top, wow, there is a table with emotional appeal will taken on the eyes ~Whether chasing popular or grasp the trend, if you good "color" believers, this pair of glasses Rainbow will add the finishing touch for you, it the most economical way to meet your requirement for changing color.

在不用的时候,它的各个部件可以收缩折叠,聚拢在一起,成为一个卵圆形的东西,其表面柔滑而富有光泽,这也就是它会被命名为"Dining Pod"的原因;而在用餐的时候,只需要将椅子拉出,将顶部升起,哇,一个非常有情调的餐桌便出现在了眼前~不管是追逐流行还是把握趋势,你若是好"色"之徒,这副彩虹眼镜将为你画龙点睛,它以最经济的方式满足你的换色要求。

After linearisation by restriction enzyme PacI, the recombinant plasmids were transfected into HEK 293-cells and amplified to give large quantities of infectious recombinant adenovirus carrying the sequences of si-FOXO1s, designated Ad-si-FOXO1-1, Ad-si-FOXO1-2, Ad-si-FOXO1-3, Ad-si-FOXO-pan and Ad-NC, respectively.

人工合成si-FOXO1s的cDNA序列,退火形成的双链cDNA克隆到腺病毒表达载体pAdTrack-CMV上,经PmeI线性化后与pAdEasy-1细胞内同源重组,最后在HEK293细胞中包装为重组腺病毒,分别命名为Ad-si-FOXO1-1、Ad-si-FOXO1-2、Ad-si-FOXO1-3、Ad-si-FOXO-pan和Ad-NC。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统:运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞:用含有400ug/mlG418的F12培养基培养14天,筛选出稳定阳性表达克隆,RPMI1640培养基扩增获得稳定表达细胞株,并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02,表达率分别为53.7%、54.5%;应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株;RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株,裂解细胞得到CD59蛋白质;通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达;50mM核糖培养72小时,获得突变人CD59糖化细胞株,BCECF染料释放试验结果显示,PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低,未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

This sort of idealism is just the reverse of that which was held by the philosophers of antiquity and their Christian successors; it does away with the reality of ideal principles by confining them exclusively to the thinking subject; it is a spurious idealism which deserves rather the name "phenomenalism"(phenomenon,"appearance", as opposed to noumenon,"the object of thought").

这种唯心主义仅仅是个逆向的那举行,由哲学家,仿古和他们的基督教接班人,它摒弃了现实的理想原则局限於他们专门为思维主体,它是一种似是而非的唯心论,值得宁愿命名为" phenomenalism "(现象,"外观",作为反对本体,"对象思想")。

The walls of the picture hall are covered with 130 paintings by eminent western European artists of the 17th and 18th centuries.

凯瑟琳一世的女儿伊丽莎白·彼得罗夫娜女皇为表示对母亲的敬意,将这座宫殿命名为凯瑟琳宫

Columbus chartered one seventy-five-foot ship, the Santa Maria, and the Spanish city of Palos supplied two smaller caravels, the Pinta and Nina.

哥伦布租用了一条75英尺长的船,它命名为Santa Maria,西班牙城市Palos提供俩条较小的帆船,船名为Pinta和Nina。

He named"polonium"in honour of her motherland -Poland .

为向她的祖国———波兰表示敬意,她把这种矿物质命名为"钋"。

In it, he planned to investigate the history of "the procreative drive" and that of prostitution. The work was never completed, but in his sketch Humboldt, for the first time, introduced a morally neutral classification of human sexual behavior according to its four possible objects

不过,遵照现时的科学术语规则,我们将把第二和第三个分类命名为异性性交和同性性交 [1](前缀" hetero -"和" homo -"在希腊语里,准确的含义为"不同的"和"相同的")。

Quesnay (from whom the idea of universal free trade originated)was the first who extended his investigations to the whole human race, without taking into consideration the idea of the nation. He calls his work 'Physiocratie, ou du Gouvernement le plus avantageux au Genre Humain,' his demands being that we must imagine that the merchants of all nations formed one commercial republic.

首先把研究扩展到全人类、不以国家概念为考虑对象的是魁奈,普通自由贸易这个概念也是从他开始的,他把他自己所写的那本书命名为《重农主义,或最有利于人类的支配力量》,他要求我们必须具有这样的想法:所有各个国家的商人是处于一个商业联邦之下的。

In 1993, an organization calling itself the Dec. 6 Coalition set up a revolving fund for women leaving violent situations to establish themselves and their children in a safer, more secure environment.

一九九三年,一个命名为"十二·六联盟"的组织,设立了一个周转基金,帮助妇女远离暴力的环境,为自己和孩子建立一个更安全、和谧的世界。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?