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Understanding how to create naming architectures is how CoupName makes naming not just unique, but organized, understandable and defendable.

正是理解如何创立命名体系结构,CoupName制造不仅独特的,而且是条理化的,容易理解的和防御性的命名

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein(KLK1gene,hK1protein).Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.

目的 将人组织激肽释放酶(基因命名为KLK1,酶命名为hK1)cDNA克隆入大肠杆菌表达质粒,以重组菌表达的GST-hK1融合蛋白免疫小鼠,获得了分泌hK1特异单克隆抗体的杂交瘤细胞株。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein(KLK1gene,hK1protein).Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.Specificity of the monoclonal antibody was shown by Western blotting and by immunofluorescence.Results The monoclonal antibody reacted specifically to E.coli-expressed hK1and with the KLK1cDNA-transfected COS-1cells.

目的 将人组织激肽释放酶(基因命名 KLK1,酶命名为hK1)cDNA克隆入肠杆菌表达质粒,以重组菌表达的GST-hK1融合蛋白免疫小鼠,获得了分泌hK1特异单克隆抗体的杂交瘤细胞株方法将KLK1cDNA克隆入真核表达质粒,用获得的重组质粒转染COS-1细胞,经间接免疫荧光试验证明,上述单克隆抗体能与转染细胞发生特异反应。

From here, we will rename some of the nodes and transitions to make the process more readable. To do this, click on the node and the name will become enterable, or double click and use the dialog to enter the new name.

单击节点选中它,再单击一次,就可以重命名此节点;或者双击节点,在弹出的对话框中也可以重命名节点;或者在屏幕下方的属性窗口重新指定名称。

JCM11314〓 and Flavobacterium omnivorum (Type strain ZF-8〓, AS1. 2747〓=JCM11313〓) are proposed for the two new isolates. Purification and characterization of a cold-adapted protease from ZF-8〓.

因此,命名新种ZF-4和ZF-6〓为Flavobacterium xinjiangensis(模式菌株为ZF-6〓,AS1.2749〓=JCM11314〓);命名新种ZF-8〓为Flavobacteriumomnivorum(模式菌株为ZF-8〓,AS1.2747〓=JCM11313〓)。

The gene cloned from gibel carp is named Carassius auratus gibelio hira and that from coloreded crucian carp is named Carassius auratus hira .

从银鲫中新克隆的基因被命名为Carassius auratus gibelio hira,从彩鲫中新克隆的基因被命名为Carassius auratus hira。

Because of the priority of the publication for bacterial names, it be named as Burkholderia gladioli and B. gladioli ATCC 10248 was the type strain of the new combined species.

根据细菌命名的优先原则命名这个新的种为唐菖蒲伯克霍尔德氏菌,B.gladioli ATCC10248是它的模式菌株。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的,我们设计了以下的实验步骤﹔(1)二次代谢物回收及检测样本处理:我们将发酵所得的培养上清液,利用疏水吸附性树酯Amberlite XAD-2吸附回收,并以甲醇溶离及真空减压浓缩脱水等方式处理,以取得提供抗氧化活性筛选之检测样本;(2)抗氧化活性检测及菌种筛选:以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用;经此筛选程序,我们选获了具有抗氧化物质高生产力的菌株,命名为AMBL-029C;(3)抗氧化物质的分离纯化:针对生产菌株的发酵回收处理液,以矽胶薄层色层分离法经物质层析纯化后,并以高效能液相层析法(High performance liquid chromatography;HPLC)分析物质可得一初级纯化物质,命名为AMBL-029C-TS;(4)抗氧化物质的物理化学性质分析:由酸碱转溶(acid-base fractionation)实验得知,此抗氧化物质属於中低极性的强酸性物质,对温度(37℃-100℃)及酸碱度(pH3.0-13.0)均表现出高稳定性;(5)在抗氧化机制探讨方面,我们针对数种不同的抗氧化机制进行探讨,即: 1。

F, architectural style is to Cao Wei during the period of architectural styles on the basis, thus making a name / f,""汉风.contiouos the door of a large Brown Wall carving is members of the three Kingdoms period liubei and thought" Dongfang hero of the historical allusions," in the lobby of the right side is named " hero Pavilion " themselves mainly to providing customers with buffet breakfast, utencils, bars and other services, which could accommodate 60---90 people left and right; the left hand side is named " are the youyan tanghang isogenous sctudy diningcn," can accommodate more than 100 people at the same time, mainly to provide you with these dishes, Cantonese, peasant cuisine in a variety of cuisine, mainly as a zero.the first floor and procudtion 30per epidifferentiability between the different styles of gatherings, you can provide you with the wrong way of tea, catering, services, and 汉风/ f, there are various specifications of the Conference room, there are a number of accommodating more than 200 people of muiti, there are dozens of people can fit in, you can meet your different meetings and hospitality requirements.

汉风楼的建筑风格是以曹魏时期的建筑风格为基础,故起名&汉风楼&。迎门的大型古铜色的壁雕就是大家所熟知的三国时期刘备与曹操&青梅煮酒论英雄&的历史典故,大厅右侧是命名为&英雄阁&的西餐厅,主要为顾客提供自助早餐、西餐、酒吧等服务,可容纳60---90人左右;左手边是命名为&许都宴堂&的中餐厅,可容纳100多人同时就餐,主要为您提供湘菜、粤菜、农家菜等多种菜系,主要为散客提供零点服务。一楼和二楼有30余间风格不同的贵宾厅房,可以为您提供餐饮、茶艺、棋牌等服务,而且汉风楼还有各种规格的会议室多间,有同时容纳200多人的多功能厅,还有可以容纳几十人的中、小型会议室,可以满足您不同的会议及接待需求。

In general, overall nucleotide sequence identity between two distinct viruses are less than 89%, and strains of virus must have sequence identity greater than 89%, so VA2 is a distinct begomoviruses. In accordance with the revised proposal for naming geminiviruses, we propose to name VA2 as Malvastrum yellow vein virus.

根据&双生病毒科病毒全基因组核苷酸序列同源性小于89%,往往定名为不同病毒;大于89%,则认为是同一病毒的不同株系&这一原则,VA2为双生病毒的一种新种,根据病毒分类委员会命名原则,我们将该病毒命名为赛葵黄脉病毒,英文名为Malvastrum yellow vein virus,简称MYVV。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。