味细胞

The mechanism of PPS accelerating IEC-6 cells migration was also researched with gene-chip technique. The Altas Clontech Rat cDNA Chip which consisted of 1176 spots representing different functional categories such as heat shock/stress, cell cycle, transcription factors, hormones, metabolism, etc was used. Result showed in cell migration model of IEC-6 cell line, compared with control group, expression of 38 genes in total 1176 genes varied in cells treated by PPS, including 21 genes up-regulated and 17 genes down-regulated. Date analyze showed the mechanism of PPS accelerating IEC-6 cells migration mainly related with promoting the numbers and activity of cellular ion channel, especially 〓 channel.

从以上实验结果中我们可以得出以下结论：四君子汤总多糖具有促进IEC-6细胞增殖、迁移、粘附的作用；四君子汤总多糖无论是对IEC-6细胞增殖、或者迁移的作用均优于各单味药多糖，可能是各种单味药多糖综合作用的结果；多糖可能是四君子汤"益气健脾"药效的主要成分；小肠上皮细胞可能是四君子汤总多糖的作用靶点之一，提示临床采用四君子汤及"益气健脾"法治疗"脾虚证"的疗效机制可能与增强机体的整体免疫和肠道粘膜免疫功能有关。

Surface of gill arch(Bar = 100μm);Fig. 5 Support cell and taste cell composing the taste bud(Bar = 10μm);Fig.

Bar =10μm ；图4 鳃弓表面味蕾的形态(Bar =100μm)；图5 构成味蕾的支持细胞和味细胞表面形态(Bar =10μm)；图6

Methods:Preparing pastille serum of Rehmanniae pill of Six Ingredients with low,medium and high doses and using 10% pastille serum with different doses to cultivate OS-732 cells.

制备低、中、高剂量的六味地黄丸含药血清。10%低、中、高剂量的六味地黄丸含药血清培养OS-732细胞，显微镜下观察细胞生长情况；进行细胞计数和绘制生长曲线；用MTT法检测细胞增殖；进行细胞碱性磷酸酶活性测定。

Experimental data were acquired by LabVIEW and processed through the time-domain analysis and frequency-domain analysis with Matlab. We analyzed qualitatively the specific effect of different tastants and proved the sensitivity to salt and sourness of taste receptor cells isolated from rat fungiform papilla. These experimental results demonstrate the feasibility that the cell chip based on LAPS can be used in the study of taste transduction mechanism.

采用LabVIEW采集数据、Matlab进行时域和频域分析，我们定性分析了不同味质作用于味觉细胞后胞外电信号的变化，证实了大鼠菌状乳头的味觉细胞对于咸味和酸味的敏感性，实现了细胞芯片在味觉传导机理研究中的应用。

according to the mechanism of bitter formation, the bitter taste masking technology is classified into different sorts from different taches that disturb the bitterness formation, include adding the taste correcting substance to confuse the cerebral taste; staving or preventing the drug releasing in mouth and segregating the contact between drug and taste buds; anesthetizing the taste cell to enhance the threshold of bitter taking; preventing bitter taste receptor combining with bitter blocker, cutting off the signal transduction pathways etc.summerize the methods of taste masking technology,besides,describe the method's advantages and disadvantages, and the technology of preparation.provide academic direction for the reasonable use of bitterness taste masking.

依据苦味形成机理，从干扰苦味形成的不同环节将中药苦味掩味技术分为添加矫味剂，混淆大脑味觉掩盖药物苦味；延缓或阻止药物在口腔释放，隔离药物和味蕾的接触；麻痹味觉细胞，提高苦味感受阈值；阻止苦味受体与苦味剂的结合，切断信号传导掩盖苦味等4类，综述掩盖中药苦味的方法，并简述各方法的优缺点和制备技术，为合理应用苦味掩味技术提供理论指导。

Methods: NG108-15 cells were cultured in vitro at 37℃in a humidified atmospherecontaining 5％CO_2. Aβ_(25～35) fragment was dissolved with deionized distilledwater and aged for 4 days at 37℃. The cells were exposured to 5μmol/LAβ_(25～35) for 24h to establish the cell model. Twelve rabbits were divided intotwo groups at random, and garaged with MWD or physiological saline controlrespectively for 7 days to collect two kinds of cerebrospinal fluid, theyare CSF from rabbits treated with MWD and CSF from rabbits treated withphysiological saline.

NG108-15细胞在37℃、体积分数为5％的CO_2培养箱中培养，Aβ_(25～35)溶解于双蒸水中，置于37℃培养箱中老化4d，使其寡聚，毒性增强，细胞被置于浓度为5μmol／L的Aβ_(25～35)中作用24h，建立起AD细胞模型。12只清洁级大耳白家兔购于华中科技大学同济医学院动物中心，将其随机分成两组，分别给与等量的加味温胆汤和生理盐水灌胃7d，末次给药后1h内用水合氯醛将给药组白兔麻醉，1.2ml／kg，无菌条件下用7号注射针头从枕骨大孔处垂直进针，抽取清亮含药脑脊液。

Bitter compounds in saliva activate particular T2R/TRB isoforms activate gustducin heterotimers, activated α-gustducin stimulates PDE to hydrolyze cAMP, the decreased cAMP may disinhibit cyclic nucleotide-inhibited channels to elevate intracellular Ca2+.

唾液中的苦味化合物激活T2R/TRB同型体，这种激活导致味导素异聚体的激活，被激活的α-味导素又激活PDE，PDE水解cAMP，cAMP水平的降低使环核苷酸抑制的阳离子通道去抑制，从而使细胞内Ca2+升高。

Salty and sour directly transduction by apical channels that are non-gated channel or chemically-gated channel. Bitter compounds in saliva activate particular T2R/TRB isoforms which activate gustducin heterotimers, activated α-gustducin stimulates PDE to hydrolyze cAMP, the decreased cAMP may disinhibit cyclic nucleotide-inhibited channels to elevate intracellular Ca2+.

咸和酸直接通过顶部非门控或化学门控离子通道转导唾液中的苦味化合物激活T2R/TRB同型体，激活导致味导素异聚体的激活，被激活的α-味导素又激活PDE，PDE水解cAMP，cAMP水平的降低使环核苷酸抑制的阳离子通道去抑制，从而使细胞内Ca2+升高。

Bitter compounds in saliva activate particular T2R/TRB isoforms which activate gustducin heterotimers, activated α-gustducin stimulates PDE to hydrolyze cAMP, the decreased cAMP may disinhibit cyclic nucleotide-inhibited channels to elevate intracellular Ca2+.

唾液中的苦味化合物激活T2R/TRB同型体，这种激活导致味导素异聚体的激活，被激活的α-味导素又激活PDE，PDE水解cAMP，cAMP水平的降低使环核苷酸抑制的阳离子通道去抑制，从而使细胞内Ca2+升高。

Taste bulb ...

味觉细胞，味细胞？？

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。