周质的

P1 BMSCs, P3 CCs, and P3 ADAS were implanted into collagen/hyaluronic acid scaffold at different concentrations of 10%, 20%, 50%, 80%, and 100%, and the cells were then three-dimensionally cultured in serum-free culture media. Two weeks later, SO staining and immunohistochemical staining were employed to observe the synthesis between of chondroitin sulfate and type II collagen.

取P1骨髓间充质干细胞，P3软骨细胞和P3脂肪源性成熟基质细胞种植入不同比例(10%，20%，50%，80%，100%)的胶原/透明质酸支架中，在无血清培养液中三维培养。2周后，用SO染色和免疫组织化学染色分析，观察硫酸软骨素和Ⅱ型胶原合成情况。

New bone formation was observed to be increasing significamly in both DCN bone cements and DC bone cements with the passage of time. By the end of 24 weeks, new bone had bridged the gap between the proximal and distal fragments in DCN bone cements and DC bone cements. By histomorphological detection in DCN bone cements, Abundant of chondral and new bone islands were seen at the site of defects 4 weeks after the operation and a number of new bones formed and enlarged by both enchondral ossification and appositional formation by the 8th weeks.

研究发现，DCN骨水泥植入4周大量间充质细胞分化，在材料与骨端之间出现一层软骨细胞，软骨细胞继续增生并向编织骨分化；8周材料降解的同时，小梁骨出现；12周新骨长入材料并已经基本替代完成，形成初步骨性连接；24周骨缺损完成骨性连接，新骨进行结构改建，材料内部形态结构明显变化。

One week after administration of lead acetate and cadmium chloride, the numbers of NOS-positive IC reduced significantly.

给予醋酸铅和氯化镉1周后，NOS阳性的睾丸间质细胞数量明显减少，并随给药时间延长而加重，NOS活性逐渐降低，给药4周后NOS阳性的睾丸间质细胞几乎不着色。

Mitochondria was relatively little in size. Round primary lysosome with high electron-densed granules and secondary lysosome with high or low electron-densed granules were seen frequently. DCs contained many rough endoplasmic reticulum, the Golgi apparatus and ribosomes. The vacuoles with flocculent electron-densed granules were rare. Some special granules in cytoplasm were seen, whose surface like earphone were covered with a membrane. High electron-densed contents in the granules were near one side and the other side was bright. The nucleus became markedly small in volume, nephroid or hoofed in shape. The nucleus had little euchromatin and lots of heterochromatin under nuclear membrane.

子宫内膜癌组织DC超微结构特征如下：细胞形态不规则，与正常子宫内膜组织DC相比，胞膜较光滑，胞膜表面树突状胞浆突起显著减少，部分突起呈粗短状；胞质中线粒体相对少，圆形而电子密度高的初级溶酶体和不规则形且电子密度高低不一的次级溶酶体多见；高尔基体、粗面内质网、核糖体丰富；含微量絮状电子致密物的胞饮小泡显著减少；胞质中可见形态特殊的颗粒，该颗粒外周膜包裹，略呈圆形，中间部位稍弯曲，如耳机状，颗粒中由高电子致密物居于一侧，而另一侧则呈透亮状；胞核显著减小，居于胞质一侧，常呈肾形或马蹄形，核内常染色质较少，异染色质多边集于核膜下。

Results］the gross morphology of the tendons showed the experimental group had lesser adhesion and better reactiveness than that in the control group.the fibrin had degraded at 3 weeks. cell tracing showed that the labeled bone marrow derived mesenchymal stem cells remained viable and presented in the intratendinous region for at least 6 weeks, becoming more diffuse at later timepoints.at 3 weeks, collagen fibers appeared more organized and there were better morphometric nuclear parameters in the treatment group. at 6 and 12 weeks, there were no differences between the groups with regard to morphometric nuclear parameters.

结果］实验组相比对照组肌腱大体观察粘连差，肌腱活动性好。3周后纤维蛋白载体即降解，细胞示踪结果显示标记的骨髓间充质干细胞至少6周内仍可保持活性并存在于肌腱组织中，但之后逐渐扩散。3周时，实验组与对照组相比胶原纤维排列更为有序，且胞核形态结构更规则，但在6周及12周时，实验组与对照组胞核参数测定无统计学意义。3周时实验组比对照组具有更强的的生物力学特性，但之后则差别不明显。

MAIN OUTCOME MEASURES: Growth characteristics, osteoblast and adipogenic induction in vitro of peripheral blood MSCs were measured.

主要观察指标：外周血间充质干细胞的生长特性，外周血间充质干细胞体外诱导成骨、成脂能力。

Microscope examination:in group a,at 2 weeks,a few inflamatory cells appeared in the holes with many osteoblasts and ostoid appeared around the drilled holes.at 8 weeks,marrow formed in the drilled holes in group b,at 2 weeks,there were a large amount of osteoblasts in the drilled holes and some ostoid formed around thc drilled holes.at 4 weeks,the drilled holes were full of trabeculae.at 8 weeks,the trabeculae matured with the marrow appeared in the intertrabecular space.

组织学结果：2周时a组钻孔区出现少许炎症细胞，边缘出现较多成骨细胞并有骨组织形成，至8周时，钻孔区内形成骨髓组织，只在边缘形成骨小梁结构。2周时b组钻孔区有大量的成骨细胞，边缘有较多骨组织形成，4周时钻孔区内充满新生骨小梁结构，8周时钻孔区内骨小梁成熟，小梁有骨髓组织填充。［结论］骨髓基质干细胞对兔股骨头缺血性坏死有良好的修复作用。

All the transfected and un-transfected rMSCs were attached to Allo-DBMs. These new biomaterials were implanted in muscle bags and segmental radius defects of the New Zealand white rabbits, and some controlled material groups were established for comparison. All the biomaterials and the controlled materials were assessed by gross observation, radiographical and histological methods.

取兔自体骨髓基质细胞培养扩增，用脂质体介导的方法转染人骨发生蛋白2基因，将转染和未转染人骨发生蛋白2基因的自体骨髓基质细胞附和于异体兔脱钙骨基质形成新的生物植骨材料，连同空白及单纯脱钙骨基质对照组植入兔前肢肌袋和桡骨缺损。2、4、6、8周分别取材进行大体，放射线和病理观察。

TWF, lead acetate and cadmium chloride. Methods Using NADPH-d histochemistry to investigate activity change of NOS in the testis of mice, which were survived for 1,2,3,4 weeks after administration of TWF, lead acetate and cadmium chloride.

给予醋酸铅和氯化镉1周后，NOS阳性的睾丸间质细胞数量明显减少，并随给药时间延长而加重，NOS活性逐渐降低，给药4周后NOS阳性的睾丸间质细胞几乎不着色。

One week after administration of lead acetate and cadmium chloride, the numbers of NOS-positive IC reduced significantly. With prolonging administration of such metal salts NOS activity gradually reduced.

给予醋酸铅和氯化镉1周后，NOS阳性的睾丸间质细胞数量明显减少，并随给药时间延长而加重，NOS活性逐渐降低，给药4周后NOS阳性的睾丸间质细胞几乎不着色。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。