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The result of framework protein and lysosome marked with fluorescence was observed in sym-acorched laser scaning microscope: red fluorescent β-tubulin and green fluorescent eathepsin in nerve cell were seen in distribution in the same sample. The distribution of both was the same, and was located in around nucleus in the early of differentiation, and in cytoplasm and apophysis in the maturity of differentiation.

共焦激光扫描显微镜镜观察荧光双标的骨架蛋白和溶酶体的染色结果：在同一标本上，不同波长的单光激发可分别见到发红色荧光的β-tubulin和发绿色荧光的组织蛋白酶D在神经元内的存在及分布情况，二者的分布区域大致相同，神经元分化初期，较集中分布在核周附近，神经元分化成熟则分布于胞质及突起内。

Following the puncture of zona pellucida with two holes by injection pipette that contained donor nuclei or cells, the injection pipette was pulled back to the perivitelline space while the negative pressure was increased in the holding pipette until the polar body and karyoplasm were wiped off completely. Then a reconstructed embryo was completed by the direct injection of the donor nucleus or cell without pulling out the injection pipette.

首先以预先吸有细胞核或细胞的注射针在固定于持卵针上的卵母细胞透明带上穿刺两个孔，然后一边缓慢地将注射针回拔至卵周隙中，一边逐渐增加持卵针中的负压，直至极体与目标核质被完整吸入持卵针中而完成去核，最后在不拔出注射针的情况下直接注射细胞核或完整细胞进而完成重构胚的构建。

For long-term treatments, the rat administered daily by gavages for six weeks, the results of OGTT showed that guava fruit water or alcohol extracts could reduce the plasma glucose level in the diabetic Wistar rats, however there was on significant difference in insulin level among all tested groups. The results of carbohydrate metabolism showed diabetic Wistar rats administered with 200 or 400 mg/kg BW of guava fruit water or alcohol extracts could decrease more than 15% glucose-6-phosphatase and fructose-1,6-bisphosphatase activities, and it also increase more than 12% glucose-6-phosphate dehydrogenase activities to reduce inhibitory hexokinase from reducing glucose-6-phosphate level.

在醣类代谢方面，实验结果发现糖尿病鼠灌食番石榴果实水及乙醇萃取物六周后，肝脏醣质新生作用中丙酮酸合成肝醣之关键酵素glucose-6-phosphatase及fructose-1，6-bisphosphatase的酵素活性会降低15%以上；另一方面肝脏glucose-6-phosphate dehydrogenase酵素活性则明显增加12%以上，并造成glucose-6-phosphate浓度减少，进而降低其对hexokinase的抑制(因为hexokinase受其代谢产物G-6-P的浓抑制)，因此加速肝脏葡萄糖的糖解作用。

After 3 weeks of continuous observation, we found the infection efficiency still keeping in the range of 80%~90%. Conclusion The lentivirus expression vector for β-catenin was successfully constructed.

在感染慢病毒质粒的人毛囊干细胞，当感染复数为10时，感染后48h的感染效率可达80%以上，感染后3周的感染效率仍维持在80%~90%。

Changes of liposome encapsulated psoralen and release rate were detected during a 3 week storage at 4 ℃ for evaluating the stability of the liposomal formulation.

以同浓度的补骨脂素凝胶为对照，用透析法检测补骨脂素脂质体凝胶的体外释药模式，并对其在4 ℃下贮存3周的释药稳定性进行研究。

Methods Solid-tumor-bearing mice were produced with Lewis lung cell and randomly divided into radiation group (n=18) and control group (n=17).The mice in the radiation group were irradiated by 30 Gy X-ray. The expressions of E-cadherin and MMP-9 were detected by immunohistochemical. Results After two weeks of therapy.

建立Lewis肺癌模型，将荷Lewis肺癌的C57BL/6雄性小鼠35只随机分为照射组(n=18)和对照组(n=17)，照射组接受30 Gy的X线肿瘤局部照射，对照组不接受照射，2周后采用免疫组化法检测瘤组织中E-钙黏素蛋白和基质金属蛋白酶－9(MMP-9)蛋白的表达。

MethodsSolidtumorbearing mice were produced with Lewis lung cell and randomly divided into radiation group (n=18) and control group (n=17). The mice in the radiation group were irradiated by 30 Gy Xray. The expressions of Ecadherin and MMP9 were detected by immunohistochemical.ResultsAfter two weeks of therapy.

建立Lewis肺癌模型，将荷Lewis肺癌的C57BL/6 雄性小鼠35只随机分为照射组（n=18）和对照组（n=17），照射组接受30 Gy的X线肿瘤局部照射，对照组不接受照射，2周后采用免疫组化法检测瘤组织中E钙黏素蛋白和基质金属蛋白酶9(MMP9)蛋白的表达。

In the SMC of the normal rabbits, myofilaments and macula densa were abundant, few mitochondrla and endoplasmic reticulums were found around the nuclear, and the SMC was in the contractive stage. In the SMC of the model rabbits, few myofilaments and macula densa were found, rough surfaced endoplasmic reticulums and mitochondria were abundant, some phagocytosed much lipid and became foam cells, the SMC was in the synthetic stage.

实验12周，高脂组家兔主动脉斑块面积百分比为47.69±12.84％，内膜斑块厚度为0.68±0.08mm，内膜斑块横断面积与中膜面积比值为1.28±0.35；内膜脂质斑块弥漫且较厚，有大量的泡沫细胞堆积，可达8～10层，管腔直径变小，内膜不光滑；泡沫细胞内富含脂滴，呈椭圆形气球样，有的斑块中可见显著的平滑肌细胞增生及大量的弹力纤维形成。

Results From the fourth week, in diabetic rats, the contents of AGEs in plasma and the abdominal aorta increased, the relative area of α-SM-actin in the abdominal aorta decreased, the density of nucleus of VSMC increased, and mitochondria and rough endoplasmic reticules in VSMC increased.

结果 从4周起，DM大鼠血浆和腹主动脉的AGEs显著增多；腹主动脉α-SM-肌动蛋白的相对面积减小，VSMC的细胞核密度增大，线粒体和粗面内质网增多。

Methods Mammalian expression vectors containing human MGMT gene were tranferred into PBMC in vitro via liposome. Realtime PCR and Western blot were used to detect the expression of MGMT at mRNA and protein levels.

利用脂质体法将含入MGMT基因的真核表达载体导入体外培养的外周血单个核细胞，实时PCR及Western blot检测目的基因mRNA及蛋白水平的表达。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。