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Before the experiment and in the second,fourth week of the experiment,blood was taken from mice's epicanthic intravenous of each group to determine biochemistry index of SCr,BUN,blood potassium,blood sodium and radioimmunity index of PCⅢ,HA,LA.

在实验前及实验的第2、4周,分别从各组大鼠眼内眦静脉采血,测定血肌酐、血尿素氮、血钾、血钠等生化指标以及Ⅲ型前胶原、血清透明质酸、层粘连蛋白等放免指标,42d时从心脏直接采血复查上述各指标,而后处死动物取肾脏进行组织形态学及特殊染色检查。

The inhibitory effect of dietary DHA on the mRNA expression of hepatic lipogenic genes was abolished when broilers fed the control diet for 2 wk.

各组肉鸡改餵饲对照组饲粮两周后,鸡只肝脏中脂质生成相关基因的表现在各组之间无显著差异。

At the 8 week postoperatively,three groups still have not restored the normal thichness of corneal epithelial cell;but in the blank group there was more neoformative irregular collagen in the anterior stromal layer.

术后4周,三组覆盖切削区的角膜上皮细胞仍有轻度增生,空白组前基质层内较多新生胶原,排列不规则,失去板层结构,瘢痕形成。

After osteoplastic induction, peripheral blood MSCs had strongly positive reactions for alkaline phosphatase staining, total collagen staining, alizarin red staining.

外周血间充质干细胞成骨诱导后,碱性磷酸酶染色、总胶原染色及茜素红染色均呈强阳性;成脂诱导后油红染色呈阳性,有一定数量的脂滴形成。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度,然后每个角膜被分成两半,共48个半片角膜,再分成4组,每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数;12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变;12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性;12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

Microbubbles attached with the naked plasmid DNA of EGFP were infused into the rat by periocular injection with destructing microbubbles by ultrasound immediately. After 2 weeks , the EGFP expression in the rats'retina were detected by confocal laser scanning microscopy .

两周后处死大鼠做冰冻切片,在激光共聚焦显微镜下观察蛋白表达情况及分析EGFP质粒在大鼠色素上皮层的表达强度。

Under scanning electron microscopy, along with the extension of the culture time, the chondrocytes grew and propagated well on the surface. On 3 weeks, the cells adhered to the surface, and extended the pseudopodia, by which the cells were connected each other. The cells secreted matrix, which deposited around the cells and the surface of meniscus. In some area, the cells formed the structure like cartilage lacuna.

扫描电镜观察,随着培养时间延长,细胞在材料表面生长良好,细胞密度增加。3周时,可见细胞黏附在材料表面,有伪足伸出,细胞间通过伪足相互连接,并可见细胞分泌的大量基质沉积于细胞周围和材料表面,部分区域形成类似于软骨陷窝样结构。

Methods:276 patients of psychogeny associated with brain disease were treated with olanzapine for 12 weeks.

对276例脑器质性精神障碍患者进行12周的奥氮平治疗,使用bprs、tess评定治疗效果,治疗前后查血常规、尿常规、肝功能、肾功能、血糖、血脂、心肌酶、心电图、脑电图、胸透检查。

Methods:276 patients of psychogeny associated with brain disease were treated with Olanzapine for 12 weeks. All subjects were evaluated with BPRS and TESS. And patients were checked blood and urine routine, liver and renal function, blood sugar, blood fat, CM enzyme, EKG, EEG, chest fluoroscopic examination.

对276例脑器质性精神障碍患者进行12周的奥氮平治疗,使用BPRS、TESS评定治疗效果,治疗前后查血常规、尿常规、肝功能、肾功能、血糖、血脂、心肌酶、心电图、脑电图、胸透检查。

Dr. Rowsey likes the conservative approach: I give my patients with mild to moderate dryness the option to test drive the punctal plugs first. The collagen plugs dissolve after about a week, which allows my patients to decide if they are beneficial.

罗塞博士喜欢用传统的方式,我给那些轻度或中度干眼症患者试用punctal塞,胶原质塞在大约一周后溶解,这可以让患者决定是否收效。

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