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Similar results were observed as those of tissue array, showing that the expression of RIG-I was reduced significantly in HCC compared with that in para-tumor and normal liver tissue samples.

上述肝癌组织芯片免疫组化检测结果提示,在肝癌患者的癌旁组织中RIG-I蛋白广泛表达,且主要在核周胞质中分布,然而,在肝癌组织中RIG-I蛋白表达明显下调。

Methods 60 patients with cardiac failure cachexia were randomly divided into two groups,including the group with nutritional support and the group without

观察60例心力衰竭恶病质患者,在心力衰竭标准治疗基础上随机将患者分成观察组和对照组,在治疗前及4周后观察两组分别在心功能状态、营养状态等指标方面的变化。

The liver fibrotic model was induced by CCl4 i.p for 5wk. Telmisartan was given at the same time model establishment in each intervened group by administration lasted for 6wk. Decollate to get the blood of all rats after 7 weeks . ALT、AST and ALB of serum was detected by automatic biochemistry equipment. Hyaluronic acid was detected by ELISA detection. Liver and spleen was obtained to calculate the index. The liver pathological changes were observed in each group of rats after HE staining to evaluate the therapeutic effect of Telmisartan; TGF-β1、PPAR-γand PDGF of liver tissue was detected by the method of immunohistochemistry respectively; The mRNA expression of TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γand iNOS was detected by RT-PCR detection, respectively.

腹腔注射CCl4制备SD大鼠肝纤维化动物模型,连续灌胃6周,处死动物取血,检测血清ALT、AST、ALB;ELISA法测定血清透明质酸;取肝、睥,并计算肝、脾指数,组织切片HE染色后进行组织病理学疗效评估;用SP免疫组化法测定肝组织TGF-β1、PPAR-γ及PDGF蛋白表达;半定量RT-PCR方法检测肝组织TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γ和iNOSmRNA的表达。

In the first week of renal obstruction, the immunohistochemical labeling of tubular VEGF was intensified, accompanied by deformation and dilation of adjacent Flk-1-positive PTC lumina, mild renal tubular cell apoptosis and minimal tubulointerstitial fibrosis.

结果: 1。肾脏梗阻第一周,肾小管上皮细胞胞浆里的VEGF染色在局部有增强,Flk-1阳性PTC数量变化不显著,肾小管上皮细胞凋亡和肾间质纤维化很少见。

MSCs did not proliferate within the alginate-GRGDY matrix during the 2 weeks examined. These results were further substantiated by concurrent cell density measurements using a hemocytometer.

在2周的检查期内,间充质细胞干细胞在甘氨酸-精氨酸-甘氨酸-天冬氨酸-酪氨酸藻酸盐环境中并没有发生增殖,这些结果在用血细胞计数器测量细胞密度时得到进一步确认。

Heterochromatin is dynamically regulated during the cell cycle and in response to developmental signals.

在细胞周期中,异染色质会被进行动态的调节,以响应发育信号。

Results: Expression of PAI-1, TIMP-1 and FN in kidney of diabetic rats began to increase in the second week of diabetes; PAI-1 and TIMP-1 mainly expressed in intracytoplasm of renal tubular epithelial cells, while FN mainly expressed in renal tubule matrix and glomerului; Their expression showed a tendency of increasing along with the progression of diabetes.

结果:PAI-1、TIMP-1及FN表达从糖尿病2周起较正常对照组明显增多(P.01),PAI-1和TIMP-1阳性表达主要位于肾小管上皮细胞胞浆内,FN阳性表达主要位于肾小管间质和肾小球,且随着糖尿病进展有逐渐增加的趋势。

Different concentrations of indomethacin were added into ten glioma cell cultures and the levels were detected after one week.

对10例胶质瘤细胞培养液中加入不同浓度消炎痛溶液,培养1周后测其上清液PGE2的含量。

RESULTS: During 10 - 12 weeks, in cuntrol group: The defect area was repaired by white and soft tissue that had no resistance to press. The repaired tissue was still lower than the surrounding articular surface with clear boundary. By histological observation, it was found that the defect was repaired by the mechanism similar to inflammatory reaction and the defect is ultimately filled by the hyperplasia of hyaline degenerative fibrous tissues. In filling group: the defect was repaired by semi-transparent, smooth, textured tissues with polish that had resistance to press as well as elasticity. The repaired tissue was almost similar to the shape of the surrounding cartilage,difficult to be distinguished. After histological observation, it was found that there was no inflammatory reaction, but active hyperplasia of inner bonetissue and cartilage tissues; a lot of osteoid tissues and trabeculation were found. Newlborn cartilage was fused with surrounding cartilage tissue and connected with surrounding tissues.

结果:10~12周,对照组:缺损区由白色、质软、按压无阻抗的组织修复,修复组织仍低于周围关节面,边界仍清晰可辨,组织学以类似炎症反应的机制修复缺损,最终以透明变性的纤维组织的增生来填补缺损部位;填充组:缺损区由半透明状、质韧光滑有光泽,按压有阻抗并有弹性的组织修复,修复组织与周围软骨外形上已基本相似,不易区分,组织学未见有炎症反应的过程,内骨组织和软骨组织增生活跃,并可见大量类骨组织和骨小梁形成,新生软骨和周围软骨组织融合,并与周围组织连接。

In our research,Wistar rats and SD rats which were parathyroidectomized were used as donors and recepients respectively.The parathyroid gland of the Wistar rat were allografted into the cerebroventricle and other different sites of the brains in SD rat s tereotaxically. Using methods of testing serum Ca~(2+) concentration,histological examination,histochemical and immunocytochemical methods and electron microscopic observation,we investigated the survival of the grafting tissue,its revascularization, infiltrating of monocytes, changes of microglia and astrocytes, the relationship of the CNS with the immune system, and the mechanism of rejection were investigated.The results as following: I .Effects on PTG after allografting into cerebroventricle and cerebral parenchyma of the brain: Testing serum Ca~(2+) conc.

本研究以Wistar大鼠作为供体,切除甲状旁腺的SD大鼠作为受体,在立体定位下将同种异体的PTG移植到大脑的不同部位,设计了侧脑室-内分泌系统移植的代替途径,比较了侧脑室内移植和脑实质内移植的异同,通过测定血清Ca~(2+)的浓度、组织学检查、组织化学和免疫细胞化学以及电镜观察对移植后PTG组织的存活、血管的再生、单核细胞的浸润、CNS内胶质细胞的变化、对免疫系统的影响及免疫排斥的机制进行了研究,结果如下:一、同种异体PTG侧脑室内移植与脑实质内移植的比较:血清Ca~(2+)的检查发现,PTG侧脑室内移植后第7天,血清Ca~(2+)的浓度恢复到8mg%以上,一直持续到移植后第8-12周;移植后第12周仍有60%移植PTG的动物血清Ca~(2+)维持在正常水平。

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