周质的

Section in situ hybridization demonstrated that the Fstl1 transcripts in cervico-lumbar spinal cord were distributed in the gray matter neuro-cells and ependymal cells. hybridization signals were present widely in dedifferentiated cells and mesenchyme cells at 2 wks and 3 wks while also in cartilaginous cells at 3 wks of regenerating blastema after tail amputation.

原位杂交结果显示，多疣壁虎脊髓中Fstl1的阳性信号主要存在于灰质神经元细胞及室管膜细胞；而在断尾再生2~3周尾芽中，Fstl1阳性信号广泛存在于再生尾芽的新生区未分化细胞及间充质细胞，3周尾芽中，阳性信号还存在于软骨生成区。

Light and electron microscopy showed central chromatolysis of motor neurons in the lumbar cord from 1 week after the start of compression.

研究结果：光学和电子显微镜检查表明从压迫1周后出现腰髓运动神经元的中心染色质溶解。3周后，在前角可见到一些神经元的凋亡。

One view is that the nucleocapsids get tegument in the nucleus, and by budding into perinuclear space, they obtain their envelope from the inner lamella of the nuclear membra...

一种意见认为，核衣壳在细胞核内获得被膜，并通过出芽在内层核膜处获得其囊膜后，进入核周间隙，进一步经与核周间隙相连接的内质网，以囊泡转运的方式经高尔基体到达细胞膜，囊泡膜与细胞膜融合，病毒颗粒释放到细胞外。

METHODS: Fresh bovine tendo calcaneus collagen was dispersed in 0.5 mol/L acetic acid, co-precipitated with chitosan and lyophilized. Dry membranes were cross-linked in 0.05% glutaraldehyde for 24 hours. In vitro its degrading rate was measured by use of collagenase degrading test. The chitosan-collagen membrane was implanted to subcutaneous dorsal sites of SD rats. After implantation, histocompatibility, vascularity and degradation were observed in vivo.

以酸溶法制备胶原溶液，添加一定比例的脱乙酰化几丁质，冻干成膜，0 。0 5 %戊二醛溶液浸泡交联，行体外酶降解实验，检测其耐受酶降解能力；将制备的几丁质-胶原蛋白膜包埋于 36只 SD大鼠皮下，术后 3、5天，1、2、3、4、6、8和 12周取材，检测其组织相容性、血管化能力及材料在体内降解情况。

Results (1) Primary callus came into being 3 days after operation. Mesenchymal cells from peripheral tissues around the primary callus were differentiated into chondrocytes 1 week later. Endochondral ossification was going on then. Bridging callus was formed in the fracture sites 4 weeks after operation.

结果 (1)伤后3 d开始形成原始骨痂。1周时肉芽组织中的间质细胞开始分化为软骨细胞，软骨形成后再进行软骨内化骨。4周时形成连接骨折端的桥接骨痂。

Majority of aderent cells from BM were fibroblast-like cells, and small parts were endothelioid cells. Aderent cells from PB and CB at the fifth generation contained more endothelioid cells and mononuclear and macrophage-like cells besides fibroblast-like cells.

初始培养和传代培养后，骨髓间充质干细胞多为成纤维样细胞，仅有少量内皮样细胞；而传至第5代的外周血、脐带血间充质干细胞除有成纤维样细胞外，还有较多的内皮样细胞和单核－巨噬样细胞。

Methods The rat models of neuroglioma were established,and divided into two groups. The rats in experimental group were lavaged with MMC 1 week after injected with C6 glioma cells, 10 mg.kg-1every day,the rats in control group were treated with sodium chloride at the same dose.

制备大鼠脑胶质瘤动物模型，将造模成功的大鼠随机分为对照组和实验组，实验组大鼠注射C6脑胶质瘤细胞1周后，每天灌胃给予10 mg.kg-1MMC，连续5 d；对照组给予相同体积的生理盐水。

The human cartilages are composed of chondrocyte and extracellular matrix , the form of chondrocytes are hypertrophy and the quantity are less; the ECM of cartilage are compised of type Ⅱ collagen and proteoglycan. Articular cartilages are all hyaline with little fibers. Trauma and arthritis are the main cause of cartilage injury, the ommilayer injury ofcartilage can be recovered by marrow, but because of without stimulation mechanism, the new tissues are merely fibrocartilages, they can not be coincide with hyaline cartilage in menchanics; the purely damage of articular cartilage can not stimulate chondrocyte to regenerate because of without blood circulation, thus, the plerosis of articular catilage can not depend on the proliferation of local chondrocyte. Ever since, people tried their best to find a way to reconstruct articular cartilage.

中文题名人骨髓基质干细胞成软骨诱导及多孔复合材料作为细胞载体的体外实验研究副题名外文题名 Cartilage induction of human mesenchymal stem cells and experiment on compound porous materials as cells' scaffold in vitro 论文作者刘晓岚导师周江南学科专业外科学研究领域\研究方向学位级别博士学位授予单位中南大学学位授予日期2003 论文页码总数68页关键词骨组织工程软骨细胞骨髓基质干细胞壳聚糖高分子外消旋聚乳酸馆藏号BSLW /2003 /R68 /10 造成人体关节软骨损伤的原因主要为创伤和关节炎，关节软骨全层损伤可由于骨髓中间充质干细胞的高速增殖修复，但这种修复由于缺乏相应的刺激机制，只能形成纤维软骨，而不能形成符合关节生理、力学要求的透明软骨；单纯软骨部分损伤软骨组织内无血管，软骨细胞迁移迟缓，无法使损伤区域软骨细胞再生，因此，关节炎及关节创伤后的软骨修复不能依赖于软骨细胞的增殖和迁移。

Drug intervention groups received either daily inhalation of budesonide, ipratropine or heparin respectively, starting on the 8th day or TGF-β1 monoclonal antibody(TB21)0.5 mg twice (6th and 19th day) via the tail veinous injection.

其它各药物干预组于制作模型第8 d起分别雾化吸入布地奈德（布地奈德组，12只）、溴化异丙托品（溴化异丙托品组，12只）和肝素（肝素组，6只）溶液。4周后检测小支气管平滑肌及胶原厚度，用免疫组化法及原位杂交法观察各生长因子在支气管肺内的表达，用放免法检测血清和BALF中细胞外基质成分Ⅲ型前胶原、层粘连蛋白及透明质酸。

Blood-brain barrier in tumors and peritumoral oedematous regions had the same variations in ultrastructure.

瘤体和瘤周水肿区血脑屏障超微结构变化无明显差异，内皮肿胀，毛细血管腔狭小，胶面有稀疏的绒毛样突起，内皮为无孔型，胞质内胞饮泡较多，内皮紧密连接增长，细胞连接间隙增宽，基膜完整，胶质膜缺损。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。