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Alternative etiologies for the development of this disorder include intrauterine insult (ischemic, metabolic, or viral such as CMV) during the 12th to 24th week of embryologic development. This results in damage to the germinal matrix and radial glial cells. Radial glial cells act as mechanical guides by which neurons are transported to the outer cortex during development.

另一种病因可能是由于第12-24周期间的宫内损伤(缺血、代谢异常、或病毒感染如CMV),其结果导致生发基质和放射状胶质细胞的损伤,在神经元移行至外皮层的过程中,放射状胶质细胞充当着机械性向导的作用。

In order to inquire the causes of occurrence of lipoid pneumonia in rat that were raised in standard SPF animal reproductive area, we used self-reproducing standard SPF rats, male 50 and female 50. All of the rats were divided into 10 groups, raised in standard Barrier system, and fed the standard diets imported from U.S.A.. All animals were divided into 4 stages to sacrifice. Autopsy, histopathological and blood biochemical examination were made at each stage. The results reveal that no lipoid pneumonia were found to occur in the 5, 10 and 26-week-old groups of male and female rats. 40% of male and 60% of female were found to occur slight focal lipoid pneumonia in the 52-week-old groups of rats.

利用成大医学院实验动物中心生产的标准无特定病原大白鼠雌、雄各50只分成10组,在屏障系统中进行实验,使用美国进口的标准实验动物饲料餵食,在一年中分四期剖检,并做病理组织学及血液生化学观察,分析比较结果显示5,10及26周龄的雌、雄大鼠皆未发生类脂质性肺炎。52周龄雌大鼠有60%,雄大鼠有40%观察到患有轻度局部性类脂质性肺炎。

Recombinant plasmid pSVH 7 DNA of avian influenza virus H7 subtype heamagglutinin gene was encapsulated with DC-chol/DOPE liposomes and PC/chol/SA liposomes separately. Two-week old SPF chickens were intramuscularly inoculated with 50 μ g/0.2ml of the liposome entrapped PSVH 7 DNA. Four-weeks later, each chicken was challenged with 0.1ml 〓 AIV . One week after the challenge, the secretion of the cloacas was collected and transfected to chicken embryos to isolate the virus. The virus was isolated from 6/6 of the control group, 1/6 of the naked DNA group, 1/6 of the PC/chol/SA entrapped DNA group and 0/6 of the DC-chol/DOPE liposome entrapped group. The HI antibody titers (log2) of the four groups were 6. 83±0.98, 7. 0±1. 26, 7. 83±1. 17 and 8. 00±0.89 respectively 1-week after challenge, and 8. 5±0.55, 8. 17±0.82, 8. 68±0.45 and 9. 33±0.54 respectively 2-week after challenge. The results showed that inoculation of liposome entrapped DNA significantly enhanced resistance to virosis in animals.

将含禽流感病毒H7亚型血凝素基因的重组质粒pSVH7用DC-chol阳离子脂质体和胆固醇/卵磷脂/十八胺脂质体包裹,免疫2周龄SPF鸡,4周后用同型禽流感病毒进行人工感染,1周后采集泄殖腔分泌物分离病毒,结果未免疫组6/6分离到病毒,裸质粒DNA免疫组1/6分离到病毒胆固醇/卵磷脂/十八胺脂质体包裹DNA免疫组1/6分离到病毒,DC-chol脂质体DNA免疫组没有分离到病毒(0/6):人工感染后1周各组的HI抗体(Log2)分别为6.38±0.98,7.00±1.26,7.83±1.17,8.00±0.89,2周后为8.50±0.55,8.67±0.82,8.68±0.45,9.33±0.52,脂质体包裹组在同期均高于未免疫组和裸DNA免疫组,表明脂质体包裹质粒DNA免疫动物后,能增加动物对病毒感染的抵抗力和反应能力。

RESULTS AND CONCLUSION: PCHC: Intra-bone mechanical strength was lower at 2 weeks, the lowest at 4 weeks, but then closed to intension of normal cancellated bone at 8 weeks, higher than normal cancellated bone at 12 weeks, and recovered to the level of normal cancellated bone at 16 weeks.

结果与结论:多孔碳酸化羟基磷灰石骨水泥:2周时的骨内力学强度较低,4周时降到最低,8周时接近正常松质骨强度,12周时超过正常松质骨强度,16周时恢复到正常松质骨水平。

A diverse range of effects:on protein solubility and secretion was observed,and these effects were highly dependent on the particular chaperone.In the host strain over-expressing chaperone SecB harboroing pSecB,the amount of total periplasmic proteins was increased by about 71%and the activities of alkaline phosphatseand GL-7-ACA acylase(GL7A)were increased by about 54%and l.5-fold respectively.In the host strain over-expressing chaperone GroEL bearing pGroEL,the amount of total periplasmic proteins was increased by 52%,and the activity of penicillin G acylasewas in creased by about 76%;about 90%of Hexameric Calcitonin(Cal6)inclusion body and l5%of MS2interleukin3(MS2-HIL3)inclusion body became soluble respectively.All the data were compared with the parental strains un-bearing plasmdis pSecB or pGroEL.

在过量表达SecB的宿主菌中,周质空间分泌蛋白总量较对照组提高了约71%,GL-7-ACA酰化酶在周质空间酶的活力较对照组提高了约1.5倍,碱性磷酸酯酶在周质空间酶的活力较对照组提高了约54%;在过量表达GroEL的宿主菌中,周质分泌蛋白总量较对照组提高了约52%,青霉素G酰化在周质空间酶的活力较对照组提高了约76%,鲑鱼降钙素六聚体的可落性组分的比例由原来的45%增加到约90%,而MS2-人白介素-3融合蛋白的包涵体有约15%转变为可溶性组份。

Most proteins destined for the periplasm and beyond are exported post-translationally by SecA.

大多数靶向细胞外周质的蛋白通过SecA的翻译后转运出。

Reconstruct ion of interstitial collagen in sinuatrial node occur since the rat diabetes has been duplicated for 16 weeks. The unbalance between collagen type Ⅰ and type Ⅲ in sinuatrial node of diabetes rat can be reversed by insulin treatment.

Ⅱ型糖尿病大鼠窦房结16周后出现间质胶原重构,胶原类型比例失衡;Ⅱ型糖尿病大鼠行胰岛素降低血糖后可不同程度下调窦房结间质的沉积,一定程度上逆转胶原类型比例紊乱。

At postnatal 1st week immunopositive reaction of NGF was detected mainly in sustentacular cells and the spermatogonia also showed positive staining. NGF positive staining in the testes was observed in interstitial cells, spermatogenetic cells, sustentacular cells and Leydig cells at 3rd week. After the postnatal 5th week, NGF-positive immunostaining was also detected in intersitial cells and spermatogenetic cells, but the intensity of reaction was weaker than that at 1st and 3rd weeks.

免疫组化定位分析显示:睾丸组织的神经生长因子蛋白表达于小鼠出生后的各个时期内,1周龄睾丸组织免疫阳性反应主要位于支持细胞,精原细胞也有着色;3周龄睾丸组织的间质细胞、各级生精细胞、支持细胞、管周肌样细胞表达均呈现阳性;5周后的睾丸组织内神经生长因子呈低水平表达,主要表达于间质细胞和生精细胞内。

Positive staining of oxytocin and vasopressin were in the cytosol of the neurons and the former gave less intensity,many positive varicosities for vasopressin were also noticed in neurite.

催产素的免疫阳性产物主要在细胞核周围的胞浆即核周质内,而加压素的免疫阳性物质除了在核周质内有很强的反应外,在突起内也可见很强的免疫反应。

It also had a pretest of object control skills in TGMD-2 (Test of Gross Motor Development 2nd Edition : including: Strike a Stationary Ball, Stationary Dribble, Catch, Kick, Overhead Throw, Underhand Roll), followed by another six weeks of lessons in games, at last there was a posttest a week after the lessons. This research analyzed the data by repeated measures t-test, and the level of significance was α=.05. Regarding quality, we promoted the teachers specialty through the process of designing games, teaching, practicing, self-examining and improving.

并於游戏课程介入前一周进行TGMD-2操作性动作技能的前测(包括定点击球、原地运球、踢球、接球、高手投掷、低手滚球),接著进行为期六周的游戏课程,且於课程结束后一周进行后测,研究所得资料以相依样本t考验进行统计分析,并将研究之统计显著水准介定在α=。05;在质的部份,藉由游戏教学设计、实践、反思、修正的历程,来提升教师之专业。

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