周质体

As a result penicillium of Ma Erni humble is double photograph bacterium, mould bacterium colony can discover the broom shape mycelial of diagnostic sex,; of pigment of rose of water-solubility of the generation after sanded Paul fosters 3 days is medullary inside and outside of cell of blood of the week outside mixing all can discover spore;PAS coloring sees bacterium body show circle, elliptic or sausage shape, size is differ, it is 2~8 μ M about, color of afterbirth wall incarnadine and clear and successive, it is thus clear that inside the cell of sausage shape one apparent horizontal stroke is lain between, afterbirth is qualitative not easy and chromatic.

结果马尔尼菲青霉菌为双相菌，霉菌型菌落可发现特征性的扫帚状菌丝，沙保罗培养3天后产生水溶性玫瑰色素；骨髓和外周血细胞内外均可发现孢子；PAS染色可见菌体呈圆形、椭圆形或腊肠状，大小不一，约为2~8μm，胞壁染红色且清楚连续，在腊肠状的细胞内可见一明显的横隔，胞质不易着色。

Following the puncture of zona pellucida with two holes by injection pipette that contained donor nuclei or cells, the injection pipette was pulled back to the perivitelline space while the negative pressure was increased in the holding pipette until the polar body and karyoplasm were wiped off completely. Then a reconstructed embryo was completed by the direct injection of the donor nucleus or cell without pulling out the injection pipette.

首先以预先吸有细胞核或细胞的注射针在固定于持卵针上的卵母细胞透明带上穿刺两个孔，然后一边缓慢地将注射针回拔至卵周隙中，一边逐渐增加持卵针中的负压，直至极体与目标核质被完整吸入持卵针中而完成去核，最后在不拔出注射针的情况下直接注射细胞核或完整细胞进而完成重构胚的构建。

Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下：一、tau蛋白过度表达和聚积对细胞形态的影响：倒置显微镜下观察两种细胞的形态，发现N2a/wt细胞的突起多而长，而N2a/tau40细胞胞体变圆，突起明显缩短；免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍，免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光，tau主要分布在核周和突起起始部分的胞质内，而N2a/wt细胞内的荧光很弱。

Research on the Iridoids composition of Valeriana jatamansi Jones in the role of mechanism of action of rat model induced by chronic stress3.1 Effection of Iridoids composition of Valeriana jatamansi Jones on 5-HT and 5-HIAA in colon,serum and central of IBS rats induced by chronic stressMethods:Animals were randomly divided into7 groups:normal group,model group, OSD group,FXT group and Iridoids composition of Valeriana jatamansi Jones groups in quantiy of 24.92mg/kg,12.46mg/kg,6.23mg/kg.

同时，蜘蛛香环烯醚萜可显著降低醋酸所致小鼠的扭体次数，说明其有良好的镇痛作用。3蜘蛛香环烯醚萜治疗肠易激综合征机理3.1蜘蛛香环烯醚萜对5-HT神经递质的影响3.1.1蜘蛛香环烯醚萜对外周和中枢5-HT含量的影响目的：探讨蜘蛛香环烯醚萜对慢性应激所致IBS大鼠结肠、血清和脑中5HT、5-HIAA的影响方法：动物随机分为对照组、模型组、昂丹司琼组、氟西汀组、蜘蛛香环烯醚萜24.92mg/kg、12.46mg/kg、6.23mg/kg组，除对照组外，其余各组进行慢性应激实验。

Research on the Iridoids composition of Valeriana jatamansi Jones in the role of mechanism of action of rat model induced by chronic stress3.1 Effection of Iridoids composition of Valeriana jatamansi Jones on 5-HT and 5-HIAA in colon,serum and central of IBS rats induced by chronic stressMethods:Animals were randomly divided into7 groups:normal group,model group, OSD group,FXT group and Iridoids composition of Valeriana jatamansi Jones groups in quantiy of 24.92mg/kg,12.46mg/kg,6.23mg/kg.

同时，蜘蛛香环烯醚萜可显著降低醋酸所致小鼠的扭体次数，说明其有良好的镇痛作用。3蜘蛛香环烯醚萜治疗肠易激综合征机理3.1蜘蛛香环烯醚萜对5-HT神经递质的影响3.1.1蜘蛛香环(来源：5bABd5C论文网www.abclunwen.com)烯醚萜对外周和中枢5-HT含量的影响目的：探讨蜘蛛香环烯醚萜对慢性应激所致IBS大鼠结肠、血清和脑中5HT、5-HIAA的影响方法：动物随机分为对照组、模型组、昂丹司琼组、氟西汀组、蜘蛛香环烯醚萜24.92mg/kg、12.46mg/kg、6.23mg/kg组，除对照组外，其余各组进行慢性应激实验。

In histopathology sections showed that alveoli were complete and clear with walls being thin and brittle. In experimental group, some petechia were observed in lung samples , the area of fibroblast and fibrinogen in histopathology sections were found , and the ground-glass predominance and reticulation structure in Micro-CT findings were increased during the period from the 1st week to the 3rd week, and degrade in the 4th week after the administration.

结果显示，控制组之肺脏检体色泽正常，没有出血，组织病理学则以I型肺泡细胞为主，无间质性肺炎且肺泡完整清净，肺泡壁薄而完整；实验组方面，第一~三周肺脏检体外观及组织病理的呈现出血点及出血病灶且越趋严重，第四周则消退，且Micro- CT影像评估亦相同。

Specimens of 8 gliomas were taken from the tumor, peritumoral oedematous region and adjacent cerebral tissue of each patient.

8例胶质瘤病例，标本分别取自同一病例的瘤体、瘤周水肿区和邻近脑组织。

Four weeks later, 69 Parkinson disease model s were randomly divided into 3 groups:①Control group(n=24):4.5 μL normal saline was injected into head of caudate nucleus of right-side corpus striatum.

取SD雄性大鼠，右侧中脑黑质致密区注入6-羟基多巴(2g/L)3μL制备帕金森病模型，4周后将69只成功帕金森病模型随机分为3组：①对照组24只：右侧纹状体尾状核头部注入生理盐水4.5μL。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。