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In this study, the effect of nCDHA on chondrogenic differentiation of rat adipose-derived adult mesenchymal stem cells in alginate gel was evaluated. nCDHA/alginate composites containing different amounts of nCDHA were prepared and used to encapsulate rat ADAS. The chondrogenic differentiation of ADAS was enhanced after 7 days and the total collagen content increased after 14 days at 500 ppm of nCDHA.

中文摘要本研究先制备一系列nCDHA/alginate奈米复合材料以三维培养方式,探讨大鼠脂肪干细胞经过ㄧ周与二周培养后,软骨分化基因及软骨基质分泌(collagen,GAG)进行评估,结果发现500 ppm nCDHA可增加第7天软骨基因分化与第14天胶原蛋白增生。

The lesion, the changes of activity of cholinesterase and acid phosphatase of motor neurons in lateral nucleus of spinal cord anterior horn, the function of axoplasm transportation and nerve conduction, the regeneration of axons and myelin sheath, and the recovery of sciatic nerve function were examined at 7, 14, 30 and 90 days intervals after operation, using Nissl and enzyme histochemistry staining, electrophysiological technique, hydrogen-peroxide oxidoreductase retrograde trace method, axon image analysis, and measurement of sciatic function index. The spinal cord anterior horn of injury side was compared to the correspondence region of spinal cord.

分别于术后7、14、30和90d应用酶组织化学方法、电生理方法、HRP (hydrogen-peroxide oxidoreductase)逆行示踪方法、轴突图象分析方法以及坐骨神经功能指数(sciatic nerve function index,SFI)测量等方法检测坐骨神经损伤后对应脊髓神经元的存活率、神经元胞体酶系变化、损伤神经在轴浆运输、电传导以及轴突、髓鞘再生等方面的恢复情况,探索外周神经损伤后OECs及几丁质对神经元的保护作用以及对新生神经功能恢复的作用,为外周神经损伤的治疗提供新的理论基础。

Section in situ hybridization demonstrated that the Fstl1 transcripts in cervico-lumbar spinal cord were distributed in the gray matter neuro-cells and ependymal cells. hybridization signals were present widely in dedifferentiated cells and mesenchyme cells at 2 wks and 3 wks while also in cartilaginous cells at 3 wks of regenerating blastema after tail amputation.

原位杂交结果显示,多疣壁虎脊髓中Fstl1的阳性信号主要存在于灰质神经元细胞及室管膜细胞;而在断尾再生2~3周尾芽中,Fstl1阳性信号广泛存在于再生尾芽的新生区未分化细胞及间充质细胞,3周尾芽中,阳性信号还存在于软骨生成区。

000 Fig.7 Tibial bone matrix of the experimental group 11thweek,the bone canaliculus turned to be illegible and the fiber scatt-ered irregularly.

标题:图7实验组11周胫骨骨细胞间质,骨小管形态模糊,间质纤维排列分布紊乱。

Cholecystokinin is a brain-gut peptide with broad biological activities. It is one of the main regulating hormones in the digestive system, and it plays an important physiological role in the central and peripheral nervous system as a neurotransmitter or a neuromodulator.

胆囊收缩素(cholecystokinin, CCK)是一种具有广泛生物学活性的脑肠肽,既是胃肠道主要调节激素之一,还广泛存在于中枢及外周神经系统中以神经递质或神经调质的形式发挥重要的生理作用。

Light and electron microscopy showed central chromatolysis of motor neurons in the lumbar cord from 1 week after the start of compression.

研究结果:光学和电子显微镜检查表明从压迫1周后出现腰髓运动神经元的中心染色质溶解。3周后,在前角可见到一些神经元的凋亡。

1 Day after injury ruptured capillary could be seen in ganglion cell layer, 4 weeks after injury cells in each layer arranged sparsely and disorderedly, in some RGCs chromatin became dense, 8 weeks after injury the cells in each layer became fewer and large amount of RGCs without nucleus could be seen.

3光镜下伤后1 d视网膜神经节细胞层出血,伤后4周视网膜各层细胞稀疏、排列欠整齐,GCL散在核染色质浓集、边聚的节细胞,伤后8周视网膜各层细胞明显减少,GCL内大量空化节细胞。

After 12 and 26 weeks of a Western diet, LDLr–/–Cat L–/– mice had significantly smaller atherosclerotic lesions and lipid cores compared with littermate control LDLr–/–Cat L+/– and LDLr–/–Cat L+/+ mice.

给予西方饮食12周及26周以后,与同窝对照LDLr–/–Cat L+/– and LDLr–/–Cat L+/+小鼠相比,LDLr–/–Cat L–/–小鼠动脉粥样硬化斑块及脂质核心明显减小。

In another experiment, after feeding APP for 5 weeks, low dosage group (15%) significantly decreased UI, liver lipid peroxidation and increased plasma calcium level compared with the negative control group. In high dosage of APP (30%), gastric mucosa and liver lipid peroxidation significantly decreased and amylase, lactase, sucrase, maltase activities significantly elevated compared to the negative control group.

另外,餵食薏仁加工产品五周后,发现低剂量组(15%)与负控制组相比能显著降低水浸润拘束紧迫法处理4小时所造成的溃疡指数,与负控制组相比显著降低肝脏脂质过氧化物含量并增加血清钙浓度,高剂量(30%)下显著比负控制组降低胃黏膜及肝脏脂质过氧化物含量,并且有提高淀粉酶、乳糖酶、蔗糖酶、麦芽糖酶活性。

The human cartilages are composed of chondrocyte and extracellular matrix , the form of chondrocytes are hypertrophy and the quantity are less; the ECM of cartilage are compised of type Ⅱ collagen and proteoglycan. Articular cartilages are all hyaline with little fibers. Trauma and arthritis are the main cause of cartilage injury, the ommilayer injury ofcartilage can be recovered by marrow, but because of without stimulation mechanism, the new tissues are merely fibrocartilages, they can not be coincide with hyaline cartilage in menchanics; the purely damage of articular cartilage can not stimulate chondrocyte to regenerate because of without blood circulation, thus, the plerosis of articular catilage can not depend on the proliferation of local chondrocyte. Ever since, people tried their best to find a way to reconstruct articular cartilage.

中文题名人骨髓基质干细胞成软骨诱导及多孔复合材料作为细胞载体的体外实验研究副题名外文题名 Cartilage induction of human mesenchymal stem cells and experiment on compound porous materials as cells' scaffold in vitro 论文作者刘晓岚导师周江南学科专业外科学研究领域\研究方向学位级别博士学位授予单位中南大学学位授予日期2003 论文页码总数68页关键词骨组织工程软骨细胞骨髓基质干细胞壳聚糖高分子外消旋聚乳酸馆藏号BSLW /2003 /R68 /10 造成人体关节软骨损伤的原因主要为创伤和关节炎,关节软骨全层损伤可由于骨髓中间充质干细胞的高速增殖修复,但这种修复由于缺乏相应的刺激机制,只能形成纤维软骨,而不能形成符合关节生理、力学要求的透明软骨;单纯软骨部分损伤软骨组织内无血管,软骨细胞迁移迟缓,无法使损伤区域软骨细胞再生,因此,关节炎及关节创伤后的软骨修复不能依赖于软骨细胞的增殖和迁移。

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I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时,滚道或滚动元件褪色是很常见的特征。

The lawyer's case blew up because he had no proof.

律师的辩护失败,因为他没有证据。