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周质

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One week after administration of lead acetate and cadmium chloride, the numbers of NOS-positive IC reduced significantly.

给予醋酸铅和氯化镉1周后,NOS阳性的睾丸间质细胞数量明显减少,并随给药时间延长而加重,NOS活性逐渐降低,给药4周后NOS阳性的睾丸间质细胞几乎不着色。

Mitochondria was relatively little in size. Round primary lysosome with high electron-densed granules and secondary lysosome with high or low electron-densed granules were seen frequently. DCs contained many rough endoplasmic reticulum, the Golgi apparatus and ribosomes. The vacuoles with flocculent electron-densed granules were rare. Some special granules in cytoplasm were seen, whose surface like earphone were covered with a membrane. High electron-densed contents in the granules were near one side and the other side was bright. The nucleus became markedly small in volume, nephroid or hoofed in shape. The nucleus had little euchromatin and lots of heterochromatin under nuclear membrane.

子宫内膜癌组织DC超微结构特征如下:细胞形态不规则,与正常子宫内膜组织DC相比,胞膜较光滑,胞膜表面树突状胞浆突起显著减少,部分突起呈粗短状;胞质中线粒体相对少,圆形而电子密度高的初级溶酶体和不规则形且电子密度高低不一的次级溶酶体多见;高尔基体、粗面内质网、核糖体丰富;含微量絮状电子致密物的胞饮小泡显著减少;胞质中可见形态特殊的颗粒,该颗粒外周膜包裹,略呈圆形,中间部位稍弯曲,如耳机状,颗粒中由高电子致密物居于一侧,而另一侧则呈透亮状;胞核显著减小,居于胞质一侧,常呈肾形或马蹄形,核内常染色质较少,异染色质多边集于核膜下。

Results (1)Astrocytes and oligodendrocytes in mixed culture appeared better growth and differentiation than the respectively purified neuroglial, especially for the oligodendrocytes.(2) There were many pseudopod process among the astrocytes, which appeared centripetal growth mode.(3) The passaged astrocytes kept good abilities of division and proliferation and completely fused after 1 week of growth, and the purity was over 95%.

结果 (1)混合培养的星形胶质细胞和少突胶质细胞生长分化状况均优于纯化后的生长,以少突胶质细胞表现更为明显;(2)星形胶质细胞呈向心性生长,细胞相邻的接触面有较多的伪足;(3)传代培养的星形胶质细胞保持较强的分裂增殖能力,可在1周左右生长至完全融合,其纯度可达95%以上。

Results]the gross morphology of the tendons showed the experimental group had lesser adhesion and better reactiveness than that in the control group.the fibrin had degraded at 3 weeks. cell tracing showed that the labeled bone marrow derived mesenchymal stem cells remained viable and presented in the intratendinous region for at least 6 weeks, becoming more diffuse at later timepoints.at 3 weeks, collagen fibers appeared more organized and there were better morphometric nuclear parameters in the treatment group. at 6 and 12 weeks, there were no differences between the groups with regard to morphometric nuclear parameters.

结果]实验组相比对照组肌腱大体观察粘连差,肌腱活动性好。3周后纤维蛋白载体即降解,细胞示踪结果显示标记的骨髓间充质干细胞至少6周内仍可保持活性并存在于肌腱组织中,但之后逐渐扩散。3周时,实验组与对照组相比胶原纤维排列更为有序,且胞核形态结构更规则,但在6周及12周时,实验组与对照组胞核参数测定无统计学意义。3周时实验组比对照组具有更强的的生物力学特性,但之后则差别不明显。

In order to inquire the causes of occurrence of lipoid pneumonia in rat that were raised in standard SPF animal reproductive area, we used self-reproducing standard SPF rats, male 50 and female 50. All of the rats were divided into 10 groups, raised in standard Barrier system, and fed the standard diets imported from U.S.A.. All animals were divided into 4 stages to sacrifice. Autopsy, histopathological and blood biochemical examination were made at each stage. The results reveal that no lipoid pneumonia were found to occur in the 5, 10 and 26-week-old groups of male and female rats. 40% of male and 60% of female were found to occur slight focal lipoid pneumonia in the 52-week-old groups of rats.

利用成大医学院实验动物中心生产的标准无特定病原大白鼠雌、雄各50只分成10组,在屏障系统中进行实验,使用美国进口的标准实验动物饲料餵食,在一年中分四期剖检,并做病理组织学及血液生化学观察,分析比较结果显示5,10及26周龄的雌、雄大鼠皆未发生类脂质性肺炎。52周龄雌大鼠有60%,雄大鼠有40%观察到患有轻度局部性类脂质性肺炎。

Microscope examination:in group a,at 2 weeks,a few inflamatory cells appeared in the holes with many osteoblasts and ostoid appeared around the drilled holes.at 8 weeks,marrow formed in the drilled holes in group b,at 2 weeks,there were a large amount of osteoblasts in the drilled holes and some ostoid formed around thc drilled holes.at 4 weeks,the drilled holes were full of trabeculae.at 8 weeks,the trabeculae matured with the marrow appeared in the intertrabecular space.

组织学结果:2周时a组钻孔区出现少许炎症细胞,边缘出现较多成骨细胞并有骨组织形成,至8周时,钻孔区内形成骨髓组织,只在边缘形成骨小梁结构。2周时b组钻孔区有大量的成骨细胞,边缘有较多骨组织形成,4周时钻孔区内充满新生骨小梁结构,8周时钻孔区内骨小梁成熟,小梁有骨髓组织填充。[结论]骨髓基质干细胞对兔股骨头缺血性坏死有良好的修复作用。

When initial NO3--N was leached, the correlation coefficients between soil initial NO3--N and mineral N extracted by CaCl2 before aerobic incubation with raygrass nitrogen uptake were decreased to 0.613 and 0.607, respectively, and both reached 5% significantly level. However, the correlation coefficients between mineralizable N extracted by aerobic incubation, soil initial mineral N and mineralizable N extracted by aerobic incubation, N0 and soil initial mineral N and N0 with raygrass nitrogen uptake were raised obviously, and all reached 5% and 1% significant level.

以不包括土壤起始NO3--N盆栽试验植物吸氮量为参比,通气培养前CaCl2淋洗起始NO3--N和起始矿质氮与五期黑麦草地上部氮素累积量间的相关性尽管有所降低,但相关性仍达5%显著水平,相关系数分别为0.613和0.607;而通气培养30周矿化氮素、土壤起始矿质氮+通气培养30周矿化氮素、N0及N0+起始矿质氮与五期黑麦草地上部吸氮量的相关系数却明显提高,相关系数分别为0.718,0.782,0.688 和0.640,均达5%或1%显著水平。

The results indicated that when initial NO3--N was not leached, soil initial NO3--N and mineral N extracted by CaCl2 before aerobic incubation were closely related with raygrass nitrogen uptake, the correlation coefficients were 0.856 and 0.862, respectively, both reached at 1% significant level. When initial NO3--N was leached, it was no good relationship between mineralizable N extracted by aerobic incubation, soil initial mineral N and mineralizable N extracted by aerobic incubation, N0 and soil initial mineral N and N0 with raygrass nitrogrn uptake, the correlation coefficients were 0.410, 0.553, 0.492 and 0.419, respectively, indicating that the poor effects of mineralizable N on reflecting soil N capacity were influenced and disturbed by initial nitrate.

以包括土壤起始NO3--N盆栽试验植物吸氮量为参比,通气培养前CaCl2所淋洗起始NO3--N和起始矿质氮与5期黑麦草地上部氮素累积量密切相关,相关系数分别为0.856和0.862,达1%显著水平;与此相反,通气培养30周所矿化氮素、土壤起始矿质氮+通气培养30周矿化氮素、氮素矿化势(N0)及N0+起始矿质氮与5期黑麦草地上部氮素累积量间无显著相关关系,相关系数分别仅为0.410、0.553、0.492和0.419。

Ovarian stromal flow PSV, EDV and perifollicular vascularity are highly interrelated with ovarian response. Ovarian response will be improved when ovarian blood velocity increases and perifollicual blood flow is abundant. Follicular fluid VEGF level was obviously higher in poor responder group, which suggests VEGF be secreted increasing for compensating due to reduced ovarian stromal blood velocity and fewer perifollicular vascularity.

卵巢基质内血流的PSV、EDV和卵泡周血流分布、卵泡液VEGF水平与卵巢的反应性密切相关,卵巢基质内血流速度升高、卵泡周边血流丰富,有助于提高卵巢的反应性;低反应卵巢组卵泡液VEGF水平显著升高,推测是由于其卵巢基质内血流速度低,卵泡周血管分布少,导致VEGF代偿性分泌增加。

In Wistar rats, latent period of a-wave in scotopic rod response after 1-week transplantation was shorten (P.05); and latent period of a-wave in scotopic rod response after 2-week transplantation was prolonged (P.05); while latent period of b-wave in scotopic maximum response after 1-week transplantation was shorten (P.05). In RCS rats, amplitude of a-wave in scotopic rod response and photopic response after 4-week transplantation was increased (PO.05); while latent period of a-wave in scotopic rod response after 4-week transplantation was shorten (PO.05).Conclusion 1. At the age of 3 months, photoreceptors of the RCS rat degenerated completely, which leaded to the reduction of amplitudes of a-wave and b-wave.

Wstar大鼠术后1周的暗适应视杆反应,a波潜伏期缩短o叼。05);术后2周的暗适应视杆反应,a波潜伏期延长o功刀5):术后1周的暗适应最大反应,b波潜伏期缩短o功刀5卜*CS大鼠术后4周的暗适应视杆反应和明适应反应,a波振幅增大o功刀匀;术后4周的暗适应视杆反应,a波潜伏期缩短o<0卜结论1、RCS大鼠在3月龄时感光细胞变性死亡,导致FERG的a、b波振幅明显降低;视网膜下腔仍存在感光细胞外节盘膜碎屑,外丛状层缺乏感光细胞的突触末端,同时Muler细胞出现反应性胶质纤维增生。

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