周质

The immunohistochemical location of noncollagenous protein, bone sialoprotein , osteopontin and osteocalcin at different stages following periradicular surgery was undertaken. The crystals formation on surfaces of MTA was evaluated when treated in different conditions without cells in vitro and in vivo. MTA was used to manage clinical problems, including perforations in roots or furcations, and pulpless permanent teeth with incomplete root formation. The study included the following three parts

本研究试图通过体内外研究探讨MTA表面牙骨质形成的机理，动物体内动态观察根尖周组织在用MTA倒充填后的组织反应和细胞外基质非胶原蛋白的表达，体外观察MTA在不同的环境下表面的形态及成分变化，和在体内环境下材料表面磷灰石层的形成，同时结合临床病例观察其临床治疗效果。

Dr. Rowsey likes the conservative approach: I give my patients with mild to moderate dryness the option to test drive the punctal plugs first. The collagen plugs dissolve after about a week, which allows my patients to decide if they are beneficial.

罗塞博士喜欢用传统的方式，我给那些轻度或中度干眼症患者试用punctal塞，胶原质塞在大约一周后溶解，这可以让患者决定是否收效。

Rowsey likes the conservative approach: I give my patients with mild to moderate dryness the option to test drive the punctal plugs first. The collagen plugs dissolve after about a week, which allows my patients to decide if they are beneficial.

罗塞博士喜欢用传统的方式，我给那些轻度或中度干眼症患者试用punctal塞，胶原质塞在大约一周后溶解，这可以让患者决定是否收效。

Results The bletilla colloid was a homogenous viscous colloid whose relative viscosity was 2324.6 mm2/s. It was easily injected through 4-F catheter and hyperattenuating under fluoroscopy, meanwhile, with good histocompatibility and hemo-compatibility, without pyrogenetic response and toxicity. In vitro, the mixture of bletilla colloid and MMC did not produce separation and suspention phenomena but released 50%of MMC at 1.8h and 100%at 3.4h. The bletilla colloid mainly embolized peripheral arteries, maintaining occlusion for 5 weeks and without formation of collateral circulation. The injuries of normal hepatic tissues were slight, without hepatic cytonecrosis.

结果 (1)白芨胶为一种均质的胶状物，有一定粘滞性，相对粘度2 324.6 mm2/s，不透X线，易经4F导管注射；(2)白芨胶具有良好的血液相容性和组织相容性，无致热原性及毒性作用；(3)白芨胶-丝裂霉素C体外混合后，无分层及悬浮现象，释放MMC 50%约需1.8小时，100%需3.4小时；(4)白芨胶主要栓塞末梢小动脉，栓塞牢靠，维持时间可达5周，不易形成侧支循环；(5)白芨胶栓塞对正常肝组织损伤轻微，未出现肝细胞坏死。

When BMSCs proliferated to enough cell number (7×106). 500 000 cells were centrifuged to a pellet and cultured in chondrogenic medium supplemented with DMEM high-glucose, insulin, transferring, selectin, pyroracemic acid, 2-pyrrolidine carboxylic acid, BSA, dexamethasone and ascorbic acid for 6 weeks.

当培养的骨髓基质干细胞达到7×106个细胞时，将每500 000个细胞离心制成高密度细胞微球，在含有DMEM高糖、胰岛素、转铁蛋白、选择素、丙酮酸、脯氨酸、 BSA、地塞米松、抗坏血酸的促软骨细胞分化培养液培养6周。

When BMSCs proliferated to enough cell number (7×10^6). 500 000 cells were centrifuged to a pellet and cultured in chondrogenie medium supplemented with DMEM high-glucose, insulin, transferring, selectin, pyroracemic acid, 2-pyrrolidine carboxylic acid, BSA, dexamethasone and ascorbic acid for 6 weeks.

当培养的骨髓基质干细胞达到7×10^6个细胞时，将每500000个细胞离心制成高密度细胞微球，在含有DMEM高糖、胰岛素、转铁蛋白、选择素、丙酮酸、脯氨酸、BSA、地塞米松、抗坏血酸的促软骨细胞分化培养液培养6周。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

DATA SYNTHESIS:（1）MSCs are especially rich in myeloid tissue and also present in placent, amniotic fluid, umbilical vein subendothelial layer, peripheral blood, liver, fat, muscles and skin.

资料综合：①间充质干细胞在骨髓组织中的含量最为丰富，此外还存在于胎盘、羊水、脐静脉内皮下层、外周血及肝脏、脂肪、肌肉、皮肤等多种组织中。

Results On conventional CT, pulmonary window revealed widespread intraalveolar calcifications of both lungs concentrating in the subpleural parenchyma of the middle and lower lobes.

结果常规CT表现：肺窗示肺实质内有无数细小散在的粟粒结节，以中下肺的外周部密集，其CT值为200～400HU，多合并不同程度的肺气肿及间质纤维化；纵隔窗示细结节影最密集区常呈沿胸膜的线带状或散在的点簇状钙化，形成&火焰征&及&白描征&。

D for 2 weeks could not remarkably affect osteogenic relative genes of bone trabecula and osteogenic differentiation of BMSCs.

d2周不能显著影响幼鼠骨小梁骨形成相关因子表达以及骨髓基质干细胞成骨分化。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。