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The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜,用茜素红-台盼蓝染色染色行角膜内皮细胞计数;用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变;用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性;实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变;并于正常对照组角膜比较。

Eighteen (72%) chronic cases showed a pattern resembling usual interstitial pneumonia, but, in most cases, with more peribronchiolar fibrosis than one would expect in UIP.

慢性患者中有18例(72%)的组织结构类似寻常型间质性肺炎,但与UIP不同的是,绝大多数病例细支气管周纤维化病灶不只一处。

The normal mechanical properties of the chondrocytes and the pericellular matrixis significant for maintaining the healthy articular cartilage.

软骨细胞和细胞周基质(pericellular matrix, PCM)的力学特性对于关节软针的生理功能具有重要的意义。

Microbubbles attached with the naked plasmid DNA of EGFP were infused into the rat by periocular injection with destructing microbubbles by ultrasound immediately. After 2 weeks , the EGFP expression in the rats'retina were detected by confocal laser scanning microscopy .

两周后处死大鼠做冰冻切片,在激光共聚焦显微镜下观察蛋白表达情况及分析EGFP质粒在大鼠色素上皮层的表达强度。

These results can lead to the following conclusion:〤GRP synthesized by the motoneurons may be a self-serving neurotrophic factor after axotomy.(2) CGRP may serve as a "injury signal" activating both the central and peripherical gli

本研究结果提示,运动神经轴突损伤后,运动神经元合成分泌的CGRP对神经元自身具有直接的神经营养作用,CGRP还可以作为损伤神经元分泌的&损伤信号&激活中枢和外周胶质细胞,从而为运动神经元的存活和轴突再生创造有利的微环境。

Methods : The levels of fasting blood glucose and fast plasma insulin, the activities of superoxide dismutase and glutathione peroxidase and level of lipid peroxidate were measured in the pancreas, heart and liver of normal and streptozotocin-induced diabetes-prone mice exposed to multiple stressors for 6 weeks. Results : The FBG level in STZ mice was elevated by stress, while the FINS level was not increased.

用链脲佐菌素复制糖尿病倾向小鼠模型,予以6周慢性多相性应激,检测其空腹血糖、空腹胰岛素水平和胰、心、肝组织超氧化物歧化酶、谷肽甘肽过氧化物酶活性及脂质过氧化物含量。

Optical control of the primary step of photoisomerization of the retinal molecule in bacteriorhodopsin from the all-trans to the 13-cis state was demonstrated under weak field conditions (where only 1 of 300 retinal molecules absorbs a photon during the excitation cycle) that are relevant to understanding biological processes.

在弱场条件下(整个兴奋周期中,300个视黄醛分子中只有一个吸收光子),细菌视紫红质中视黄醛分子的从全反式到13-顺式状态的光致异构化的最初阶段的光学控制被阐明,这与生物学过程的理解有关。

Under scanning electron microscopy, along with the extension of the culture time, the chondrocytes grew and propagated well on the surface. On 3 weeks, the cells adhered to the surface, and extended the pseudopodia, by which the cells were connected each other. The cells secreted matrix, which deposited around the cells and the surface of meniscus. In some area, the cells formed the structure like cartilage lacuna.

扫描电镜观察,随着培养时间延长,细胞在材料表面生长良好,细胞密度增加。3周时,可见细胞黏附在材料表面,有伪足伸出,细胞间通过伪足相互连接,并可见细胞分泌的大量基质沉积于细胞周围和材料表面,部分区域形成类似于软骨陷窝样结构。

Methods:276 patients of psychogeny associated with brain disease were treated with olanzapine for 12 weeks.

对276例脑器质性精神障碍患者进行12周的奥氮平治疗,使用bprs、tess评定治疗效果,治疗前后查血常规、尿常规、肝功能、肾功能、血糖、血脂、心肌酶、心电图、脑电图、胸透检查。

Methods:276 patients of psychogeny associated with brain disease were treated with Olanzapine for 12 weeks. All subjects were evaluated with BPRS and TESS. And patients were checked blood and urine routine, liver and renal function, blood sugar, blood fat, CM enzyme, EKG, EEG, chest fluoroscopic examination.

对276例脑器质性精神障碍患者进行12周的奥氮平治疗,使用BPRS、TESS评定治疗效果,治疗前后查血常规、尿常规、肝功能、肾功能、血糖、血脂、心肌酶、心电图、脑电图、胸透检查。

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