周质

The 40 included articles involved mesenchyma stem cells obtained from marrow, muscle, the navel blood, placenta, peripheral blood, adipose tissue, vessel and so on.

符合纳入标准的40篇文献中，分别涉及骨髓、肌肉、脐血、胎盘、外周血、脂肪组织、血管及其他来源的间充质干细胞。

The induced MSCs were found to be positive for desmin and specific myoglobulin of the skeletal muscle.

MSCs免疫组织化学法测定CD44反应阳性，胞质呈棕褐颗粒，核周明显，CD34呈阴性反应；诱导后MSCs结蛋白、骨骼肌特异肌球蛋白均为阳性表达。

Results The expression of the Mahoganoid protein and its mRNA was showed by brown staining and observed in the Leydig cells, spermatogonia, primary spermatocytes, spermatids, Sertoli cells, and peritubular myoid cells of testicular tissues and principal cells, basic cells and epithelial cells of epididymal tissues.

结果1。免疫组化法检测Mahoganoid蛋白的实验结果显示的棕色阳性信号可见于各天龄段大鼠睾丸的生精细胞、支持细胞、间质细胞和管周肌样细胞以及附睾输出管的高柱状、低柱状上皮细胞和附睾管的上皮细胞、主细胞、基细胞。其中Mahoganoid蛋白主要表达于胞膜和胞浆。

At the 8 week postoperatively,three groups still have not restored the normal thichness of corneal epithelial cell;but in the blank group there was more neoformative irregular collagen in the anterior stromal layer.

术后4周，三组覆盖切削区的角膜上皮细胞仍有轻度增生，空白组前基质层内较多新生胶原，排列不规则，失去板层结构，瘢痕形成。

The mouse was carried out half nephric duct ligation to induce the renal interstitial fibrosis animal model,afterwards,to set up three groups,control group,sham operation group(intragastric administration with normal sodium according to 10ml/kg weight),treatment group...

采用小鼠单侧输尿管结扎法诱导肾间质纤维化的动物模型，用盐酸青藤碱治疗，并设对照组、假手术组(小鼠每日按10m l/kg体重用生理盐水灌胃，直至治疗结束)、治疗组(选用正清风痛宁缓释片按每日30mg/kg体重给小鼠灌胃，连续3周，)，检测血尿素氮、血肌酐、血清层粘连蛋白、纤维连接蛋白的含量，免疫组化法测肾脏组织转化因子β1(TNF-β1)的表达并进行肾脏病理学的观察。

In the central, 5-HT can induce the release of inhibitory neurotransmitter γ-aminobutyric acid etc. through 5-HT2A receptor resulting in analgesia. In the peripheral, 5-HT can active the nociceptor and advance the traumatic information transmission.

在中枢可以通过2A受体引起抑制性神经递质γ-丁氨酸等的释放，从而发挥镇痛作用；在外周参与伤害性感受器的活化，促进伤害性信息的传递。

MethodsSodium Hyaluronate was injected into the palatoglossal arch, palatopharyngeal arch and root of the tongue of eight Wistar rats. An electroencephalogram, electromyogram and oronasal airflow were recorded by PSG before and 4 weeks after injection when rats were under light anesthesia, which was expected to be similar to the sleep state.

方法将8只Wistar大鼠的双侧舌腭弓、咽腭弓及舌根处注射透明质酸钠，分别于注射前及注射后4周，以浅状态模拟睡眠状态，用多导睡眠仪监测动物的脑电、肌电及口鼻气流。

METHODS: A total of 50 white rabbits were randomly divided into anterior cruciate ligament transection group, medial collateral ligament transection + meniscectomy group and normal control group. The specimens were harvested at weeks 1, 2, 3, 4, and 5 after operation. The osteocartilaginous section of the medial femur condyles were observed by gross observation, histological examination, and Markin scores. The contents of proteoglycan and collagen fiber were also measured.

取大耳白兔50只，随机分为3组前交叉韧带切除组、内侧副韧带+半月板切除组分别切除右后肢前交叉韧带、右后肢内侧副韧带和半月板，正常对照组不干预，分别在建模1，2，3，4和5周后分批于膝股骨内髁软骨取材，进行软骨组织大体和组织学观察、软骨损伤程度Markin评分；测定软骨组织蛋白多糖及软骨基质中胶原纤维含量。

After osteoplastic induction, peripheral blood MSCs had strongly positive reactions for alkaline phosphatase staining, total collagen staining, alizarin red staining.

外周血间充质干细胞成骨诱导后，碱性磷酸酶染色、总胶原染色及茜素红染色均呈强阳性；成脂诱导后油红染色呈阳性，有一定数量的脂滴形成。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。