周质

Encapsulated psoralen were evaluated by dialyzation using psoralen gel as the control. Changes of liposome????encapsulated psoralen and release rate were detected during a 3????week storage at 4 ℃ for evaluating the stability of the liposomal formulation.

以同浓度的补骨脂素凝胶为对照，用透析法检测补骨脂素脂质体凝胶的体外释药模式，并对其在4 ℃下贮存3周的释药稳定性进行研究。

Results We can induce MSCs into osteoblasts which can express gene col Ⅰ and ALP. Through analysis growth curve, we demostrateed the diphosphonate or assemble flavone of drynaria rhizome with 10^(-4)~10^(-6)mmolL^(-1) had the inhibition on the osteomast; it with 10^(-8)mmolL^(-1) had a promotion on the osteoblast; it with 10^(-10)~10^(-12)mmolL^(-1) had a restraint on osteoblast. Conclusion Through gene analysis, we found the diphosphonate or assemble flavone of drynaria rhizome can have the promotion on the osteoblast.

结果 骨髓基质细胞经过4周诱导可转化为表达colⅠ和ALP的成骨细胞，经过生长曲线分析，二磷酸盐和骨碎补总黄酮10^(-4)~10^(-6)mmolL^(-1)时，对诱导后的成骨细胞有抑制作用；10^(-8)molL^(-1)有促进作用；10^(-10)~10^(-12)mmolL^(-1)不明显，其中10^(-8)mmolL^(-1)作用最强；基因分析后，发现二磷酸盐和骨碎补总黄酮促进成骨细胞表达colⅠ和ALP，且两者合用效果大于单独用药。

The cleavation rates and blastocyst development rates of cloned embryos were used to assess the efficiency of different operational procedure. Finally, the best combination of operational procedure, that the spindle-viewer system was used for oocytes enucleating, and donor cell was electrofused into ooplasm by electrical pulse (1.9 kV/cm, 10 ms, two) to reconstruct bovine cloned embryos.

以核移植胚胎的卵裂率、囊胚发育率作为检测指标，对不同的方法所获得的克隆胚胎的卵分裂率与囊胚发育率进行比较，最后筛选获得1个优化的牛体细胞核移植操作程序，即采用Spindle view系统对牛卵母细胞进行去核操作，将供核体细胞注射到卵周隙，然后通过电融合法将供体核引入去核卵细胞质(电融合参数为1.9 kV/cm，脉冲时程10 ms，方波2次间隔2 s)。

METHODS: To construct recombinant expression plasmid subtype H1 of SIV HA gene of A/Swine/Guangdong/LM/2004(H1N1).BALB/c mice of 6-8 weeks old were immunized endemically with the recombinant plasmid.The splenocytes from the immunized mice were fused with Sp2/0 myeloma cells after the last immunization.

构建 H1 亚型猪流感病毒A/Swine/Guangdong/LM/2004(H1N1)HA基因表达质粒，基因免疫6～8周龄雌性BALB/c小鼠，加强免疫后取其脾细胞与骨髓瘤细胞 Sp2/0进行融合。

Similar expression of BMP-2, TGF-β,bFGF between the test groups and control groups was noted. Inflammatory cells in the hematoma expressed bFGF and proliferating periosteal cells, endosteal cells, medullary mesenchymal cells expressed TGF-β at 3 days postoperatively.

实验侧和对照侧3天时血肿中炎性细胞时即开始表达bFGF，增殖骨膜、骨内膜、骨髓间质细胞表达TGF-β。1周时骨端骨细胞、骨痂成骨细胞才开始表达BMP-2，同时也表达TGF-β。

The mice inoculated with Coxiella burnetii intrana- sally developed interstitial pneumonia,while the primary pathological changes of mice inoculated intraperitoneally are granulomas in spleen and liver.2.The pathological changes became more severe followed the dosage increasing.3.Coxiella burnetii can be detected in spleen and liver at day 2 after inoculation.the lesion became more and more serious from day 2 to day 12.The characteristic changes were observed at day 7,and recovered at day 14. 4.The reticuloendothelial system are main target of Coxiella burnetii.The pathogen was detected in cytoplasm of monocyte -macrophages of spleen, liver, lung, and endothelioid cells of blood vessel. 5. Coxiella burnetii can be found in macrophages lysosomes by electron microscopy. Most of them are round or rod, and polymorphic shape can also be observed in different size.

结果：1、通过不同感染途径的实验证实，滴鼻感染的小鼠主要表现为间质性肺炎，而腹腔注射感染小鼠则以脾脏、肝脏肉芽肿为主要病变。2、通过不同剂量的感染实验发现，随着感染Q热立克次体剂量的加大，动物病变愈加严重。3、通过感染后不同时间的动态病理学观察发现，在腹腔注射后第2d的脾和肝脏即可发现病原体，主要脏器的病理变化从第2d到第12d逐渐加重，第7d动物的病变最典型，至感染后14d动物的受损器官已开始出现修复性变化。4、 Q热立克次体主要侵害机体的网状内皮系统，在感染小鼠的肝、脾、肺和外周血管单核巨噬细胞以及血管内皮细胞胞浆中查见病原体。5、透射电镜观察可见Q热立克次体主要位于巨噬细胞吞噬溶酶体内，呈多形性，多见圆形和杆状，大小不一。

Among them, 39 cases were in low grade ,31 cases in high grade and 4 cases had the ependyma tumor. All the patients received radiotherapy in 3-4 weeks after operation. Thirty-one cases were performed total resection, 38 cases balk-resection and 5 cases partial resection.

对脑胶质瘤低级别39例、高级别31例及室管膜瘤4例均进行手术切除，其中肉眼肿块全切31例，大部切除38例，部分切除5例，均在手术后3~4周内接受放射治疗。

Before the experiment and in the second,fourth week of the experiment,blood was taken from mice's epicanthic intravenous of each group to determine biochemistry index of SCr,BUN,blood potassium,blood sodium and radioimmunity index of PCⅢ,HA,LA.

在实验前及实验的第2、4周，分别从各组大鼠眼内眦静脉采血，测定血肌酐、血尿素氮、血钾、血钠等生化指标以及Ⅲ型前胶原、血清透明质酸、层粘连蛋白等放免指标，42d时从心脏直接采血复查上述各指标，而后处死动物取肾脏进行组织形态学及特殊染色检查。

RESULTS: The extracellular proteoglycan can be detected as early as 3 weeks and the content increased over time.

结果：在微球培养的第3周就可以检测到细胞外基质中的蛋白聚糖，并且随着时间的增长其单位细胞的蛋白聚糖分泌量不断增加。

By the way, small uniform cells with perinuclear halos can be seen focally in otherwise classic glioblastomas.

顺便说一句，局部见到小而一致的具有核周空晕的细胞是典型的胶质母细胞瘤。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。