周质

Most proteins destined for the periplasm and beyond are exported post-translationally by SecA.

大多数靶向细胞外周质的蛋白通过SecA的翻译后转运出。

When protein folding is compromised in the periplasm of E.

当大肠杆菌E.coli周质中蛋白折叠发生阻碍，外膜蛋白OMPs的C端与三聚体DegS蛋白酶的 PDZ区域发生结合，促使ResA裂解。

The manganese protein is then transported to the periplasm having first trapped its manganese.

而后锰蛋白转移到细胞外周质，开始对锰原子进行捕获。

ELISA and MTT assay show that coexpression of 〓 gene and dsbA gene, can increase the total content of TGF β1 in the periplasm, and gain biological activities.

而ELISA测定和MTT测活都表明，共表达〓和dsbA基因，使得周质中TGFβ1的含量明显增高，并具有显著的生物学活性。

The results showed that the three scFvs specific for Xac were successfully expressed in E. coli HB2151. The molecular weight of expressed products was about 32.0×103 and the products were mainly located in the periplasm.

结果显示，3株抗柑桔溃疡病菌可溶性单链抗体在大肠杆菌HB2151中均获得成功表达，表达产物分子量约为32.0×10^3，主要集中于细菌周质腔中。

Carrying out the work in a blue-green algae, a cyanobacterium, the team has been able to show that a protein requiring copper transports to the periplasm, the outer area of the cell, where it then folds around the available metal, which is copper.

研究组已能了解到，蓝绿藻在执行生理功能的过程中，铜蛋白转移到细胞外的细胞周质，在那里对可用的金属铜进行包绕。

With the inducing time prolonged, the amount of fusion protein increased, and the highest content amounted to 39. 14% of total protein. Different temperature (15℃, 25℃ and 37℃) could induce expression of fusion protein, and the higher inducing temperature was, the more total fusion protein and soluble fusion protein was produced. Most fusion protein was expressed in the cytoplasm and was soluble, a little of fusion protein was expressed in periplasm or expressed as inclusion bodies, and no fusion protein was found in culture. Furthermore, the results of in vitro enzymatic reaction suggested that the fusion protein could catalyze the methylation of theobromine.

随着诱导时间的延长，表达的融合蛋白产量逐渐增加，表达蛋白总量最高可达到菌体总蛋白的39.14％；在15℃、25℃和37℃三个不同的诱导温度下，均能诱导产生融合蛋白，而且产生的融合蛋白的总量及其可溶性部分所占比例均随诱导温度升高而增加；在菌体的各个部位中，融合蛋白主要在细胞质中以可溶的形式进行表达，有少量在外周质中表达或以包含体形式在细胞质中表达。

After infecting E.coli HB2151 cells with one positive clone,soluble recombinant antibodies about 27 kDa were produced and located in the periplasm and the supernatant.

约27kDa大小的ER以可溶形式存在于细胞质及细胞周质中，并可分泌至上清。

Coli. Transmission electron microscopy was used to evaluate the cell morphology of both the normal and the treated E. coli. The observation with TEM suggested that silver nanoparticles lead to the formation of "pits" in cell wall of the bacteria, and silver nanoparticles could enter into periplasm through the pits and destroyed the cell membrane.

采用透射电镜观察了经纳米银粒子处理过的大肠杆菌细胞形态变化过程，结果显示纳米银粒子先在细胞壁上产生小的孔洞，通过这些孔洞进入周质空间，导致细胞膜成分渗漏和破坏细胞膜，进而进入细胞内部。

Expression level of rhGH could reach 250 mg/L culture solution by means of selecting components of original culture media, components of supplemental media, volume of supplemental media, the time of supplying supplemental media and supplying speed of supplemental media, secretion rhGH was found to represent 44%~54% of the total proteins in periplasm, and the fermentation time was reduced to 8 hrs.

通过对发酵培养基成分、补料成分、补料体积、补料时机和滴加流速的选择，分泌表达rhGH的量可达到250 mg/L，占周质蛋白的44%~54%，发酵周期缩短为8小时。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。