周质

The results were analyzed and compared. After 12 weeks, the X ray density, the torsional moment and angle in failure load test and the score of callus in histological grading were all improved in the experimental side, compared with the control side.

将外貌脱钙的同种异体骨和来历于受体的体外作育增殖的骨髓间质干细胞殽杂植入尝试侧骨缺损，比较侧仅植入同样建造的异体骨。12周后，举办X线搜检、生物力学搜检和构造学搜检，将功效举办比拟。

Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下：一、tau蛋白过度表达和聚积对细胞形态的影响：倒置显微镜下观察两种细胞的形态，发现N2a/wt细胞的突起多而长，而N2a/tau40细胞胞体变圆，突起明显缩短；免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍，免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光，tau主要分布在核周和突起起始部分的胞质内，而N2a/wt细胞内的荧光很弱。

Allogene bone marrow mesenchymal stem cells were given to mice by vena caudalis intravenous injection and D-galactose were injected into the nape subcutaneous of mice for eight weeks.

采用5% D-半乳糖（0.25ml/10g）连续8 周皮下注射建立衰老小鼠模型，并于模型建成后给予骨髓间充质干细胞输注。

Then the caprine fetal fibroblast cells were transfected with p6.5hLF-EGFP by LipofectamineTM-2000 and selected by G418 for 3 to 4 weeks. The G418 resistant transfectants were identified by PCR and EGFP detection. The results indicated that the transgene was stably integrated into the open region of the chromatin of G418 resistant fibroblast cells.

脂质体介导法转染山羊胎儿成纤维细胞，G418抗性筛选3-4周后，经PCR扩增和报告基因EGFP表达检测，得到稳定整合外源基因的转基因供体细胞系，为制备高效表达人乳铁蛋白的转基因山羊乳腺生物反应器提高可靠的核移植供体细胞。

MSCs capsulated in alginate microspheres were induced by TGF-β3 and IGF-I, serving as the experiment group. While those cultured without TGF-β3 and IGF-I were taken as controls.

实验组用转化生长因子β3和胰岛素样生长因子Ⅰ联合定向诱导藻酸钠微球包被的骨髓间充质干细胞3周，对照组培养条件中无转化生长因子β3和胰岛素样生长因子Ⅰ。

After 3 weeks, some cells turned to round shape and secreted metachromatic matrix. The cartilaginoid grafts composed of chondrogenic MSC.

向MSC培养液内加入地塞米松、转化生长因子-β1(TGF-β1)和维生素C进行软骨起源诱导培养3周，部分细胞开始转变为圆形并分泌基质。

Tapetum cells degenerated and only ER small vesieles were the visible organelle at the 2—celled pollen stage.

绒毡层在小孢子液泡化中期开始退化，开始退化的绒毡层表现为内质网腔膨大，核周腔增大，核孔数增加。

Capsule ellipsoid, thinly walled, membranous, circumscissile.

蒴果椭圆体，薄壁，膜质，周裂。

The results by invert embedding TEM showed that higher invasive CCL229cells had vast of ER,which distributed throughout cytosol especially largeamount of vesicle-like and flatten cisternal rER in pseudopodia or cell processesand membranous flow-like structure from perinuclear region to pseudopodia,and less Golgi complex.

倒置包埋法常规透射电镜对人大肠癌细胞的超微结构观察结果显示，高侵袭力的CCL229细胞中内质网数量较多并呈全细胞分布，尤其在细胞伸出的众多伪足中含有丰富的小泡状和扁平囊样粗面ER结构，而且观察到由小泡形成的&膜流样&结构从核周一直深入到伪足中，但细胞中高尔基器少见。

Four weeks after implantation, type I collagenous matrices were completely filled with cartilage-like tissue.

植入4周后，I型胶原基质充满了软骨样组织。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。