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Cortex nerve cell could be orientation differentiation to cholinergic neuron,β-tubulin was expression during the process of differentiation, which was distribution from around nucleus intensive in the early of differentiation to presented in cytoplasm in network, and extension into apophysis after maturity.

皮层神经干细胞可定向分化为胆碱能神经元,且在分化过程中细胞骨架蛋白β-tubulin同时亦有表达,由分化早期的核周密集分布至分化成熟后的胞质内网状分布,并伸至突起中。

Cathepsin D and β-tubulin marked with fluorescence were followed in distribution observed by laser scaning microscope, the distribution was from nucleus nearby to cytoplasm and apophysis, which suggested that they took part in traffic in cells.

荧光双标组织蛋白酶D及β-tubulin,共焦激光扫描显微镜下观察,可见二者伴随分布,均由开始的核周分布明显到散在分布于胞质及突起中,说明溶酶体与骨架蛋白密切相关,提示共同参与细胞内的运输。

The result of framework protein and lysosome marked with fluorescence was observed in sym-acorched laser scaning microscope: red fluorescent β-tubulin and green fluorescent eathepsin in nerve cell were seen in distribution in the same sample. The distribution of both was the same, and was located in around nucleus in the early of differentiation, and in cytoplasm and apophysis in the maturity of differentiation.

共焦激光扫描显微镜镜观察荧光双标的骨架蛋白和溶酶体的染色结果:在同一标本上,不同波长的单光激发可分别见到发红色荧光的β-tubulin和发绿色荧光的组织蛋白酶D在神经元内的存在及分布情况,二者的分布区域大致相同,神经元分化初期,较集中分布在核周附近,神经元分化成熟则分布于胞质及突起内。

Similar results were observed as those of tissue array, showing that the expression of RIG-I was reduced significantly in HCC compared with that in para-tumor and normal liver tissue samples.

上述肝癌组织芯片免疫组化检测结果提示,在肝癌患者的癌旁组织中RIG-I蛋白广泛表达,且主要在核周胞质中分布,然而,在肝癌组织中RIG-I蛋白表达明显下调。

Blood-brain barrier in tumors and peritumoral oedematous regions had the same variations in ultrastructure.

瘤体和瘤周水肿区血脑屏障超微结构变化无明显差异,内皮肿胀,毛细血管腔狭小,胶面有稀疏的绒毛样突起,内皮为无孔型,胞质内胞饮泡较多,内皮紧密连接增长,细胞连接间隙增宽,基膜完整,胶质膜缺损。

Results Group C had the symptoms such as reduced activity, acceded, indulge in lying and weight loss after 3 weeks of immune injection, 14 out of 16 SD rats in Group B had the same symptoms as Group C after 4~5 weeks of immune injection, the serum enzymes in model groups increased significantly compared with those of the control group, model group C was much higher than model group B; the duration shorted, amplitude decreased, multiphase wave increased in electromyogram of model groups; MRI examination revealed samples from model group B and C had one positive case each, which presented T1MI isodensity or hypodensity signal, T2MI and STIR serial hyperdensity signal, revealing muscle inflammation; all rats'skeletal muscle from model group C and 11 out of model group B had pathological changes, which exhibited striated muscle focal fiber degeneration, necrotized and inflammatory cell infiltration, interstitial vessel wall thickening, random cardiac muscle samples had 3 positive changes, which had similar changes to skeletal muscle, there was 1 positive change from lung sample.

结果:模型C组于免疫注射第3周左右开始出现活动减少,倦怠嗜卧,食欲体重下降等表现,模型B组有14只SD大鼠于免疫注射第4~5周出现上述症状,较C组为轻;模型组肌酶谱中,肌酸激酶、乳酸脱氢酶、谷草转氨酶与对照组比较明显升高,模型C组较模型B组升高更显著;模型组肌电图时限缩短,波幅降低,多相波增多;磁共振检查模型B组和C组选送标本中各有一例有阳性改变,表现为T1MI等或稍低信号,T2MI及STIR序列为高信号,提示肌肉炎症水肿改变;模型B组有11只,模型C组全部大鼠骨骼肌出现病理改变,表现为横纹肌局灶性分布的肌纤维变性炎细胞浸润,间质小血管壁增厚、扩张,随机选送的心肌标本中有3例有阳性改变,表现与骨骼肌相仿,选送的肺标本中有1例有阳性改变,表现为蛋白渗出,炎症细胞浸润和小血管改变。

Following successful modeling, rats of bFGF group were intratracheally injected with 400 U bFGF and rats of VEGF group with 2 μg VEGF, once a week for three times. MSCs group was injected 1 mL suspension of 4×109/L MSCs into tail vein. MSCs+VEGF group was injected MSCs into tail vein and intratracheally injected VEGF (2 ug, three times) at the same time. Model control and normal control groups were intratracheally injected with equal volume of sodium chloride.

成功造模后,碱性成纤维细胞生长因子组气管内注入400 U碱性成纤维细胞生长因子,血管内皮生长因子组气管内注入2 μg血管内皮生长因子,1次/周,共3次;单纯细胞移植组于尾静脉注入4×109 L-1骨髓间充质干细胞悬液1 mL;血管内皮生长因子+细胞移植组气管内注入血管内皮生长因子的同时,尾静脉注入骨髓间充质干细胞;模型对照组、正常对照组给予相同体积的生理盐水。

In our research,Wistar rats and SD rats which were parathyroidectomized were used as donors and recepients respectively.The parathyroid gland of the Wistar rat were allografted into the cerebroventricle and other different sites of the brains in SD rat s tereotaxically. Using methods of testing serum Ca~(2+) concentration,histological examination,histochemical and immunocytochemical methods and electron microscopic observation,we investigated the survival of the grafting tissue,its revascularization, infiltrating of monocytes, changes of microglia and astrocytes, the relationship of the CNS with the immune system, and the mechanism of rejection were investigated.The results as following: I .Effects on PTG after allografting into cerebroventricle and cerebral parenchyma of the brain: Testing serum Ca~(2+) conc.

本研究以Wistar大鼠作为供体,切除甲状旁腺的SD大鼠作为受体,在立体定位下将同种异体的PTG移植到大脑的不同部位,设计了侧脑室-内分泌系统移植的代替途径,比较了侧脑室内移植和脑实质内移植的异同,通过测定血清Ca~(2+)的浓度、组织学检查、组织化学和免疫细胞化学以及电镜观察对移植后PTG组织的存活、血管的再生、单核细胞的浸润、CNS内胶质细胞的变化、对免疫系统的影响及免疫排斥的机制进行了研究,结果如下:一、同种异体PTG侧脑室内移植与脑实质内移植的比较:血清Ca~(2+)的检查发现,PTG侧脑室内移植后第7天,血清Ca~(2+)的浓度恢复到8mg%以上,一直持续到移植后第8-12周;移植后第12周仍有60%移植PTG的动物血清Ca~(2+)维持在正常水平。

GFAP+ astrocytes hhd no changes and there were no VIM+ astrocytes surrounding wounds in postmortem groups.

死后脑干损伤组GFAP+星形胶质细胞无改变,创缘及创周无VIM+星形胶质细胞出现。

Results: From the second days of the operation, the brain swellen overtly. RBC and phagocyte can be seen in the cerebral interstitial space. The adventitia of the brain arteries and the perivascular Virchow ̄Robin space dilated irregularly. The neuronal number of hippocampal CAI sector decreased evidently. In some neuron both the nucleus and cytoplasm condensed, chromatin gathered near the nuclear membrane.

结果: 自术后第二天可见脑组织水肿,红细胞溢出及吞噬细胞浸润,小血管壁外膜及Virchow ̄Robin间隙增宽,内含大量水肿液,海马CAI区正常神经元数量明显下降,且排列及不整齐,细胞皱缩,核固缩、深染,核染色质聚集,核周囊膨大,胶质细胞增生。

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