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Then rAAV-DJ-1 was transfected into HEK-293 cells for packaging. AAV vectors encoding human DJ-1 protein were stereotactically injected into the rat SN 4 weeks prior to rotenone lesion in SN. Tyrosine hydroxylase protein was determined using immunohistochemical staining. Ethovison software allowed measurement of movement distance in 30 minutes.

并转染HEK-293细胞,进行腺相关假病毒颗粒包装,所得病毒颗粒注射到大鼠脑内感染黑质神经细胞;4周后,注射鱼藤酮于大鼠黑质相同脑区;通过免疫组织化学方法鉴定TH蛋白表达情况,采用动物行为轨迹分析软件计算大鼠30 min内运动距离。

RESULTS: After six weeks of implantation, x-ray film showed high-density signal, osteoid tissue formed under histological examination. Large amount of new bone were formed and connected to trabecularism 8 weeks after implantation in the experimental group.

结果 实验组6 周时大体标本浅红色, X 线片有较高密度阻射影像,组织学检查可见有新骨形成; 8 周时大体标本呈红色,质硬, X 线阻射影像密度更高,镜下可见大量新骨形成并相互连接成骨小梁样结构,骨细胞位于陷窝中。

RESULTS: During 10 - 12 weeks, in cuntrol group: The defect area was repaired by white and soft tissue that had no resistance to press. The repaired tissue was still lower than the surrounding articular surface with clear boundary. By histological observation, it was found that the defect was repaired by the mechanism similar to inflammatory reaction and the defect is ultimately filled by the hyperplasia of hyaline degenerative fibrous tissues. In filling group: the defect was repaired by semi-transparent, smooth, textured tissues with polish that had resistance to press as well as elasticity. The repaired tissue was almost similar to the shape of the surrounding cartilage,difficult to be distinguished. After histological observation, it was found that there was no inflammatory reaction, but active hyperplasia of inner bonetissue and cartilage tissues; a lot of osteoid tissues and trabeculation were found. Newlborn cartilage was fused with surrounding cartilage tissue and connected with surrounding tissues.

结果:10~12周,对照组:缺损区由白色、质软、按压无阻抗的组织修复,修复组织仍低于周围关节面,边界仍清晰可辨,组织学以类似炎症反应的机制修复缺损,最终以透明变性的纤维组织的增生来填补缺损部位;填充组:缺损区由半透明状、质韧光滑有光泽,按压有阻抗并有弹性的组织修复,修复组织与周围软骨外形上已基本相似,不易区分,组织学未见有炎症反应的过程,内骨组织和软骨组织增生活跃,并可见大量类骨组织和骨小梁形成,新生软骨和周围软骨组织融合,并与周围组织连接。

The figure of SFI showed that the functional recovery in the experimental groups was better than that of the control at every 10 days after sciatic nerve transection.

因此,OECs和几丁质对外周神经损伤所导致的脊髓神经元死亡和退行性变有较好的保护作用,对外周神经损伤后新生神经功能的恢复具有积极的促进作用。

Drug intervention groups received either daily inhalation of budesonide, ipratropine or heparin respectively, starting on the 8th day or TGF-β1 monoclonal antibody(TB21)0.5 mg twice (6th and 19th day) via the tail veinous injection.

其它各药物干预组于制作模型第8 d起分别雾化吸入布地奈德(布地奈德组,12只)、溴化异丙托品(溴化异丙托品组,12只)和肝素(肝素组,6只)溶液。4周后检测小支气管平滑肌及胶原厚度,用免疫组化法及原位杂交法观察各生长因子在支气管肺内的表达,用放免法检测血清和BALF中细胞外基质成分Ⅲ型前胶原、层粘连蛋白及透明质酸。

The transmission is conducted in the form of action potentials along the axon of DRG neuron to the presynaptic membrane in spinal dorsal horn. Action potential depolarizes the presynaptic membrane and activates the voltage-dependent Ca〓 channel. The influx of Ca〓 then triggers the release of neurotransmitter.

外界感觉信息以动作电位的形式沿DRG细胞轴突由外周传向DRG细胞在脊髓背角的突触前膜,导致细胞膜去极化,引起电压依赖性〓通道的开放,〓内流介导突触前膜神经递质的释放,从而完成感觉信息由外周向脊髓的传递。

It was found that in the group of allogra fts intragraft IL-2R and ICAM-1 were expressed on the POW 4 and peaked on the POW 32, and their expression levels were significantly higher than in the group of autografts and isografts.

结果发现同种异体肾移植组于第4周开始出现IL-2R及IC AM-1的表达,于32周达高峰,明显高于自体肾移植组和同质移植组。

Glass slides were used as artificial substrates for collecting ciliates from a closed intensive shrimp-farming pond near Qingdao, China.

为了解周丛纤毛虫在特定海水养殖环境中的功能与地位,作者以青岛近郊一封闭式海水对虾养殖池为采集地,采用人工基质法对周丛类纤毛虫的群落结构进行了为期140d的调查,同时开展了环境理化因子的采集和分析。

The kiln body is divided into two sections of electrical furnace body and dynamic rotation with the kiln body constructed of clayed circular hearth uniformly inserted with 9 silicon carbon rods along the circular axis( one group made up of 3 rods and Y connection for every 3 groups).

窑体部分又分为电炉炉体和动力转动两部分,炉体部分采用粘土质圆形炉膛,沿圆轴向均匀插9根硅碳棒(每三根串联成一组,三组按& Y &接线)高铝质的陶瓷炉管又置于硅碳棒内周,通电后硅碳棒产生高温,加热炉管。

Interferon-gamma upregulates interleukin-3 (IL-3) receptor expression in human endothelial cells and synergizes with IL-3 in stimulating major histocompatibility complex class II expression and cytokine production.

间充质干细胞与相关因子。中国实验血液学杂志, 2002; 10: 468-47113 朱光荣,周小玉,陆化等,人骨髓间充质干细胞表达多种造血细胞因子。

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