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At G. 32W stable expression of nov mRNA was distributed in pontine abducens nucleus, red nucleus and substantia nigra of midbrain, ventral posterolateral thalamic nucleus and mediodorsal thalamic nucleus.

第32周脑桥展神经核、中脑红核和黑质、丘脑腹外侧核和背内侧核以及第38周大脑纹状体、顶叶皮质显示nov mRNA稳定的表达。

In this study, MSCs and adipo-MSCs (MSCs cultured in adipogenic medium for 7 days) were attached to PLGA spheres and cultured for 7 days, followed by injection into nude mice for 2 weeks.

在本研究中,间充质干细胞和向脂肪方向分化的间充质干细胞(MSC在成脂培养基上培养7天)复合到PLGA材料上,同时培养7天,然后注射到裸鼠体内2周。

MIIGX3 was absorbed 4 weeks postoperatively and generally completely absorbed 12 weeks postoperatively. Injecting region of MIIGX3 was coincidence with normal cancellated bone 6 months postoperatively. The Rasmussen score was 25.8 of 20 patients after 6 months and 26.9 of 12 patients after 12 months.

术后4周高强度可注射硫酸钙开始明显吸收,术后12周基本吸收,术后6个月高强度可注射硫酸钙植入区域与正常松质骨一致。20例术后半年Rasmussen膝关节功能平均25.8分,12例术后1年Rasmussen膝关节功能平均26.9分。

Wound exudations were observed in two cases and the bacterial culture was negative. MIIGX3 was absorbed 4 weeks postoperatively and generally completely absorbed 12 weeks postoperatively. Injecting region of MIIGX3 was coincidence with normal cancellated bone 6 months postoperatively.

术后4周高强度可注射硫酸钙开始明显吸收,术后12周基本吸收,术后6个月高强度可注射硫酸钙植入区域与正常松质骨一致。20例术后半年Rasmussen膝关节功能平均25.8分,12例术后1年Rasmussen膝关节功能平均26.9分。

The total RNA from brain tissue of substantia nigra of PD rats is extracted at the 1st, 2nd, 4th and 8th week and comparison is made between it and that from normal rats. The total RNA, after RNA formaldehyde degenerated sepharose electrophoresis and separation and Northern transfer, is adsorbed unto colloxylin membrane. Then ECL labeled Selenoprotein P and an unknown gene fragment are used as probes to hybridize with the RNA on the colloxylin membrane. And the hybridized result is obtained with enzyme-linked immunosorbent assay. Finally, analysis of OD is made using ONE-Dscan software.

分别提取1周、2周、1月、2月的帕金森大鼠(各8只)黑质区脑组织总RNA,并取相对应时间的正常大鼠的脑组织总RNA作为对照,经RNA甲醛变性琼脂糖凝胶电泳分离后,通过Northern转移,将其吸附在硝酸纤维素膜上,然后利用ECL标记的Selenoprotein P和一段未知基因为探针,与硝酸纤维素膜上的RNA分子进行杂交,再通过显影取得杂交的结果,应用ONE-Dscan软件进行光密度值分析。

After varied survival periods, the model mice were sacrificed to get the specimens of SN fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry to label the DA neurons and Calbindin D28k-containing neurons with the antibodies against tyrosine hydroxylase and CR respectively. The labeled cells were counted under microscope and analyzed statistically.

模型动物存活1周后,一部分取其中脑黑质节段,固定、包埋做连续冠状石蜡切片,以免疫组织化学染色方法,显示各组酪氨酸经化酶和钙结合蛋白表达阳性细胞,光镜观察并细胞计数,统计学分析;另一部分取其中脑黑质组织匀浆,行TH、CB的Westem blot免疫印迹方法检测,用LabWorks软件分析处理。

Results (1) Primary callus came into being 3 days after operation. Mesenchymal cells from peripheral tissues around the primary callus were differentiated into chondrocytes 1 week later. Endochondral ossification was going on then. Bridging callus was formed in the fracture sites 4 weeks after operation.

结果 (1)伤后3 d开始形成原始骨痂。1周时肉芽组织中的间质细胞开始分化为软骨细胞,软骨形成后再进行软骨内化骨。4周时形成连接骨折端的桥接骨痂。

Other features of the peripheral red blood cells are protein and lipid dysglycosylation and endoplasmic reticulum double-membrane remnants.

另外的特征是外周红血细胞蛋白和脂质糖基化不良以及残留的双膜内质网,其它造血系统是正常的。

Majority of aderent cells from BM were fibroblast-like cells, and small parts were endothelioid cells. Aderent cells from PB and CB at the fifth generation contained more endothelioid cells and mononuclear and macrophage-like cells besides fibroblast-like cells.

初始培养和传代培养后,骨髓间充质干细胞多为成纤维样细胞,仅有少量内皮样细胞;而传至第5代的外周血、脐带血间充质干细胞除有成纤维样细胞外,还有较多的内皮样细胞和单核-巨噬样细胞。

RESULTS: At 16 weeks after operation, the defects were perfectly healed in the composite group, which was better than heterogenic deproteinized cancellous bone and control group with regard to the levels of bone formation, quantity of bone regeneration and reconstruction of marrow cavity.

结果:植入后16周,复合材料组骨缺损完全修复,成骨活跃程度、骨再生量和重建髓腔结构等方面均显著优于异种脱蛋白松质骨组和空白组;异种脱蛋白松质骨组,形成骨性愈合,但无髓腔再通;空白组未愈合。

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