周流的

Methods: The expression of mutated P53 gene in human lymphocytes was investigated using flow cytometry.And the micronucleus and sister chromatid exchange tests were studied in vitro.

以正常人外周血淋巴细胞为材料，用流式细胞仪检测环磷酰胺对突变型P53基因表达的影响，同时以微核及姐妹染色单体互换为指标，进行致突变研究。

The results shows that, when Re, H/D are larger, a stable vortex is generated at two sides of the convex surface.

研究结果表明当Re，H/d较大时，在凸面靶板周向两侧形成了稳定的旋流结构。

The numerical results were proved to be reliable by comparing with some published experimental data. The results show that, when only Coriolis force is in effect by omitting the centrifugal buoyancy force, the flow in the cavity is axi-symmetrically stable, the Coriolis force is a kind of resilience, and does not cause flow unstably.

研究结果表明：在哥氏力的单独作用下轴向通流旋转盘腔内的流动基本上是稳定的三维轴对称结构，即使有轻微的径向或周向振动发生，由于哥氏力是恢复力，对其有抑制作用，因此振动会随着流场的发展逐渐消失。

This piece's plot development for the around two parts, the first half description divorces many year Bai Liusu in Shanghai's maternal home full love brothers' and sisters-in-law's satire humiliation, latter half Bai Liusu to Hong Kong, has launched crosscut saw's-like love with loose prodigal son Zhou Runfa.

本片的情节发展为前后二部分，前半部描写离婚多年的白流苏在上海的娘家饱爱兄嫂的讽刺欺凌，后半部白流苏到了香港，跟风流浪子周润发展开了拉锯式的爱情。

Inductance coil is a kind of energy storage components, the current flowing through it can not mutations.

常用的电感元件有固定电感器、扼流圈、延迟线、磁头线圈、振荡线圈、中周线圈等。

The Pana volcanic rocks of the Linzizong Group in Zhaxue area, Linzhou County, Tibet are mainly composed of rhyolitictrachy lavas and volcanoclastic rocks.

分布于西藏林周县扎雪地区的林子宗群帕那组火山岩，主要由流纹质-粗安质熔岩和火山碎屑岩组成。

Objective In order to know the essential meaning of immune disorder in Recurrent Aphthous Ulcer patients and to search the agreeable treatment meanwhile, the cluster of differentiation of peripheral lymph cells were detected and analyzed by flow cytometry, and afterwards those patients were treated by immunomodulator.

目的为了更好的理解RAU患者免疫紊乱的实质及寻找有效的治疗方法，我们采用流式细胞技术，对RAU患者发病期和康复期外周血淋巴细胞的表型进行测定、分析，探索流式细胞技术在RAU的诊断及指导临床治疗方面的作用。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

The trend of doubting ancient history thought which arising in early 20th century cut away and rebated half of Chinese history,the Axial Age theory resumed the validity of the various scholars in later Zhou Dynasty,and went on disregarding the government and official scholars of the San Dai Dynasty,hut the government and official scholars of San Dai Dynasty just is the headstream of the various scholars in later Zhou Dynasty even of the whole Chinese civilization.

追溯民初以来学者早已开始的东西文明比较，更多关注的是在古埃及、古巴比伦、古印度和中华文明的第二阶段上。20世纪初兴起的疑古思潮将中国古史&砍掉一半&、&打个对折&，&轴心时代&理论则恢复了晚周诸子学的合法性，但它同时 ssbbww。 com 继续漠视三代王官学，而三代王官学正是晚周诸子学乃至整个中华文化的源头。&轴心时代&理论与中华文明对接中的偏差，其影响将是流与源的颠倒、反题与正题的倒置和各期学术史的逐次错位。

In the light of the phenomenon such as flowing sand ,and sand boil subjected from the flowing sand stratum,in the construction of artificial digging piles,the advanced support with rein- foreement and the chemical churning pile technology can be used to form the watertight curtain,thereby preventing successfully the loss of san.

文章针对挖孔桩施工中遇到流砂层，出现的流砂、涌砂现象，采用钢筋超前支护及旋喷桩技术形成隔水帷幕，成功地阻止了砂层的流失，解决了施工进度慢、不安全的难题，改善了桩周的边界条件，提高了抗滑桩的承载能力。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。